ZED1-related kinase 13 is required for resistance against Pseudoidium neolycopersici in Arabidopsis accession Bla-6

Miguel Santillán Martínez; Dongli Gao; Michela Appiano; Inge Derks; Robin P. Huibers; Glenn Spil; Xulan Wang; Richard G.F. Visser; Anne Marie A. Wolters; Yuling Bai

doi:10.3389/fpls.2023.1111322

ZED1-related kinase 13 is required for resistance against Pseudoidium neolycopersici in Arabidopsis accession Bla-6

Miguel Santillán Martínez, Dongli Gao, Michela Appiano, Inge Derks, Robin P. Huibers, Glenn Spil, Xulan Wang, Richard G.F. Visser, Anne Marie A. Wolters, Yuling Bai^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

To explore specific components of resistance against the tomato-adapted powdery mildew pathogen Pseudoidium neolycopersici (On) in the model plant Arabidopsis, we performed a disease assay in 123 accessions. When testing the resistance in the F₁ from crossings between resistant accessions with susceptible Col-0 or Sha, only the progeny of the cross between accession Bla-6 and Col-0 displayed a completely resistant phenotype. The resistance in Bla-6 is known to be specific for Pseudoidium neolycopersici. QTL analysis and fine-mapping through several rounds of recombinant screenings allowed us to locate a major resistance QTL in an interval on chromosome 1, containing two candidate genes and an intergenic insertion. Via CRISPR/Cas9 targeted mutagenesis, we could show that knocking out the ZED-1 RELATED KINASE 13 (ZRK13) gene compromised the On resistance in Bla-6. Several polymorphisms are observed in the ZRK13 allelic variant of Bla-6 when compared to the Col-0 protein.

Original language	English
Article number	1111322
Journal	Frontiers in Plant Science
Volume	14
DOIs	https://doi.org/10.3389/fpls.2023.1111322
Publication status	Published - 21 Mar 2023

Keywords

Arabidopsis
CRISPR/Cas9
powdery mildew
resistance
ZRK13

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https://edepot.wur.nl/641642Licence: CC BY

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@article{6c2575f09aa946a3b6b32bc950759f7d,

title = "ZED1-related kinase 13 is required for resistance against Pseudoidium neolycopersici in Arabidopsis accession Bla-6",

abstract = "To explore specific components of resistance against the tomato-adapted powdery mildew pathogen Pseudoidium neolycopersici (On) in the model plant Arabidopsis, we performed a disease assay in 123 accessions. When testing the resistance in the F1 from crossings between resistant accessions with susceptible Col-0 or Sha, only the progeny of the cross between accession Bla-6 and Col-0 displayed a completely resistant phenotype. The resistance in Bla-6 is known to be specific for Pseudoidium neolycopersici. QTL analysis and fine-mapping through several rounds of recombinant screenings allowed us to locate a major resistance QTL in an interval on chromosome 1, containing two candidate genes and an intergenic insertion. Via CRISPR/Cas9 targeted mutagenesis, we could show that knocking out the ZED-1 RELATED KINASE 13 (ZRK13) gene compromised the On resistance in Bla-6. Several polymorphisms are observed in the ZRK13 allelic variant of Bla-6 when compared to the Col-0 protein.",

keywords = "Arabidopsis, CRISPR/Cas9, powdery mildew, resistance, ZRK13",

author = "{Santill{\'a}n Mart{\'i}nez}, Miguel and Dongli Gao and Michela Appiano and Inge Derks and Huibers, {Robin P.} and Glenn Spil and Xulan Wang and Visser, {Richard G.F.} and Wolters, {Anne Marie A.} and Yuling Bai",

year = "2023",

month = mar,

day = "21",

doi = "10.3389/fpls.2023.1111322",

language = "English",

volume = "14",

journal = "Frontiers in Plant Science",

issn = "1664-462X",

publisher = "Frontiers Research Foundation",

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TY - JOUR

T1 - ZED1-related kinase 13 is required for resistance against Pseudoidium neolycopersici in Arabidopsis accession Bla-6

AU - Santillán Martínez, Miguel

AU - Gao, Dongli

AU - Appiano, Michela

AU - Derks, Inge

AU - Huibers, Robin P.

AU - Spil, Glenn

AU - Wang, Xulan

AU - Visser, Richard G.F.

AU - Wolters, Anne Marie A.

AU - Bai, Yuling

PY - 2023/3/21

Y1 - 2023/3/21

N2 - To explore specific components of resistance against the tomato-adapted powdery mildew pathogen Pseudoidium neolycopersici (On) in the model plant Arabidopsis, we performed a disease assay in 123 accessions. When testing the resistance in the F1 from crossings between resistant accessions with susceptible Col-0 or Sha, only the progeny of the cross between accession Bla-6 and Col-0 displayed a completely resistant phenotype. The resistance in Bla-6 is known to be specific for Pseudoidium neolycopersici. QTL analysis and fine-mapping through several rounds of recombinant screenings allowed us to locate a major resistance QTL in an interval on chromosome 1, containing two candidate genes and an intergenic insertion. Via CRISPR/Cas9 targeted mutagenesis, we could show that knocking out the ZED-1 RELATED KINASE 13 (ZRK13) gene compromised the On resistance in Bla-6. Several polymorphisms are observed in the ZRK13 allelic variant of Bla-6 when compared to the Col-0 protein.

AB - To explore specific components of resistance against the tomato-adapted powdery mildew pathogen Pseudoidium neolycopersici (On) in the model plant Arabidopsis, we performed a disease assay in 123 accessions. When testing the resistance in the F1 from crossings between resistant accessions with susceptible Col-0 or Sha, only the progeny of the cross between accession Bla-6 and Col-0 displayed a completely resistant phenotype. The resistance in Bla-6 is known to be specific for Pseudoidium neolycopersici. QTL analysis and fine-mapping through several rounds of recombinant screenings allowed us to locate a major resistance QTL in an interval on chromosome 1, containing two candidate genes and an intergenic insertion. Via CRISPR/Cas9 targeted mutagenesis, we could show that knocking out the ZED-1 RELATED KINASE 13 (ZRK13) gene compromised the On resistance in Bla-6. Several polymorphisms are observed in the ZRK13 allelic variant of Bla-6 when compared to the Col-0 protein.

KW - Arabidopsis

KW - CRISPR/Cas9

KW - powdery mildew

KW - resistance

KW - ZRK13

U2 - 10.3389/fpls.2023.1111322

DO - 10.3389/fpls.2023.1111322

M3 - Article

AN - SCOPUS:85151570624

SN - 1664-462X

VL - 14

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

M1 - 1111322

ER -

ZED1-related kinase 13 is required for resistance against Pseudoidium neolycopersici in Arabidopsis accession Bla-6

Abstract

Keywords

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