Yeast engineered for the production of 1-alcohols from sugars under anoxic conditions

The present invention relates to modified eukaryotic microbial cells that have been engineered for producing 1-alcohols and derivatives thereof under anoxic conditions. To this end the eukaryotic microbial cells have been modified to express enzymes of the fatty acid β-oxidation cycle in the cytosol of the cell, under anoxic conditions, in the absence of fatty acids and in the presence of a non-fatty acid carbon source. The cells are further modified to express a metabolic route for producing under anoxic conditions, acetyl-CoA from the non-fatty acid carbon source to feed into and drive the β-oxidation cycle in the bio synthetic direction and, to express termination enzymes for conversion of reaction β-oxidation cycle intermediates into the desired fermentation product. The invention further relates to anoxic processes wherein the cells are used to produce 1-alcohols or derivatives thereof.