Yeast engineered for the production of 1-alcohols from sugars under anoxic conditions

J.A.M. de Bont (Inventor), A. Raab (Inventor), M. Schilling (Inventor), V.A.P. Martins Dos Santos (Inventor), R. Arjona Antolín (Inventor), P. Gutiérrez Gómez (Inventor)

Research output: PatentOther research output

Abstract

The present invention relates to modified eukaryotic microbial cells that have been engineered for producing 1-alcohols and derivatives thereof under anoxic conditions. To this end the eukaryotic microbial cells have been modified to express enzymes of the fatty acid β-oxidation cycle in the cytosol of the cell, under anoxic conditions, in the absence of fatty acids and in the presence of a non-fatty acid carbon source. The cells are further modified to express a metabolic route for producing under anoxic conditions, acetyl-CoA from the non-fatty acid carbon source to feed into and drive the β-oxidation cycle in the bio synthetic direction and, to express termination enzymes for conversion of reaction β-oxidation cycle intermediates into the desired fermentation product. The invention further relates to anoxic processes wherein the cells are used to produce 1-alcohols or derivatives thereof.
Original languageEnglish
Patent numberWO2014207105
Priority date26/03/13
Publication statusPublished - 31 Dec 2014

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