The present invention relates to modified eukaryotic microbial cells that have been engineered for producing 1-alcohols and derivatives thereof under anoxic conditions. To this end the eukaryotic microbial cells have been modified to express enzymes of the fatty acid β-oxidation cycle in the cytosol of the cell, under anoxic conditions, in the absence of fatty acids and in the presence of a non-fatty acid carbon source. The cells are further modified to express a metabolic route for producing under anoxic conditions, acetyl-CoA from the non-fatty acid carbon source to feed into and drive the β-oxidation cycle in the bio synthetic direction and, to express termination enzymes for conversion of reaction β-oxidation cycle intermediates into the desired fermentation product. The invention further relates to anoxic processes wherein the cells are used to produce 1-alcohols or derivatives thereof.
|Publication status||Published - 31 Dec 2014|
de Bont, J. A. M., Raab, A., Schilling, M., Martins Dos Santos, V. A. P., Arjona Antolín, R., & Gutiérrez Gómez, P. (2014). Yeast engineered for the production of 1-alcohols from sugars under anoxic conditions. (Patent No. WO2014207105). https://worldwide.espacenet.com/publicationDetails/originalDocument?CC=WO&NR=2014207105A1&KC=A1&FT=D&ND=3&date=20141231&DB=EPODOC&locale=en_EP