Year-round screening of noncommercial and commercial oysters for the presence of human pathogenic viruses

F. Lodder-Verschoor, A.M.R. Husman, H.H.J.L. van den Berg, A. Stein, H.M.L. van Pelt-Heerschap, W.H.M. van der Poel

    Research output: Contribution to journalArticleAcademicpeer-review

    25 Citations (Scopus)

    Abstract

    Consumption of virus-contaminated shellfish has caused numerous outbreaks of gastroenteritis and hepatitis worldwide. In The Netherlands, oysters are cultured and imported both for consumption and export; therefore, the presence of noroviruses, rotaviruses, astroviruses, hepatitis A viruses, and enteroviruses was determined in 64 commercial and noncommercial oyster samples. Oysters were collected monthly for 13 months from four different harvesting areas in the Oosterschelde Delta. Oyster samples were classified by determining Escherichia coli levels according to the standards set by the Councils Directive (91/492/EEC). Two of 36 commercial and 2 of 28 noncommercial oyster samples were B-classified and therefore not ready for consumption. All other oyster samples were A-classified. For the detection of viral RNA, 150 mg of hepatopancreatic tissue was subjected to the Qiagen RNeasy Mini Kit, followed by reverse transcriptase (RT)¿PCR and Southern blot hybridization. Enterovirus RNA was detected in 14 of 64 oyster samples, of which 4 were from noncommercial oyster harvesting areas and 10 were from commercial harvesting areas. None of the other human pathogenic viruses were detected. The levels of somatic coliphages and F-specific phages were also determined in all 64 oyster samples, with some samples containing high phage levels (>50 PFU/g of hepatopancreatic tissue), but with most samples containing low phage levels (
    Original languageEnglish
    Pages (from-to)1853-1859
    JournalJournal of Food Protection
    Volume68
    Issue number9
    Publication statusPublished - 2005

    Keywords

    • hepatitis-a virus
    • polymerase-chain-reaction
    • human enteric viruses
    • norwalk virus
    • bivalve shellfish
    • escherichia-coli
    • rt-pcr
    • contamination
    • bacteriophage
    • outbreak

    Fingerprint Dive into the research topics of 'Year-round screening of noncommercial and commercial oysters for the presence of human pathogenic viruses'. Together they form a unique fingerprint.

    Cite this