Wounding-induced xylem occlusion in stems of cut chrysanthemum flowers : roles of peroxidase

W.G. van Doorn, N. Vaslier

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    47 Citations (Scopus)


    A wounding-induced xylem occlusion, resulting in severe leaf wilting, occurs in stems of cut chrysanthemum flowers (Dendranthema grandiflora), cv. Vyking. The blockage develops after about 1 h in flowers held in air at 20 °C. It is initially located in the lowermost 2 cm of the stem and upon prolonged exposure to air it is also found above 2 cm. We tested the possible role of peroxidase (EC and phenoloxidases in the blockage. Some peroxidase inhibitors (copper ions and 3-amino-1,2,4-triazole) delayed the occlusion, and treatment with compounds that inhibit peroxidase but stimulate phenoloxidase (catechol, hydroquinone, p-phenylene diamine) had the same effect. Some inhibitors of phenoloxidase (p-nitrophenol, p-chlorophenol, p-nitrocatechol, and sodium metabisulfite) also delayed the occlusion. Phenoloxidase activity in plants comprises catechol oxidase (EC and laccase (EC The blockage was considerably delayed by catechol oxidase inhibitors (tropolone and 4-hexylresorcinol), even more so than with general phenoloxidase inhibitors. The results indicate that the occlusion is mainly due to a physiological (oxidative) process, requiring both peroxidase and catechol oxidase activity
    Original languageEnglish
    Pages (from-to)275-284
    JournalPostharvest Biology and Technology
    Issue number3
    Publication statusPublished - 2002


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