White Spot Syndrome Virus (WSSV), the causative agent of White Spot Syndrome Disease (WSSD), is a major shrimp pathogen in Indonesia. WSSV is unique among shrimp pathogen because of its broad host range including Polychaetes. Dendronereis spp is a ubiquitous Polychaetes and natural food of shrimp raised in brackishwater pond in Indonesia. The objective of this research is to determine the occurrence of WSSV, the viral load and the infective tissues in Dendronereis spp obtained from the shrimp pond. Dendronereis spp was obtained with PVC (10 cm in diameter) from a traditional shrimp pond in Semarang vicinity. As a comparison, healthy looking Penaeus monodon was also obtained from the same pond. The occurrence of WSSV in Dendronereis spp was determined with 1-step and nested PCR using primer for WSSV major envelope protein, VP 28. The viral load was counted with 1-step Real Time PCR and the infective tissue was detected with immunohistochemistry using polyclonal antibody for VP 28 raised in rabbit. WSSV was detected in Dendronereis spp with 1-step and nested PCR. The point prevalence of WSSV infection in Dendronereis spp is 90 %. The viral load ranged from 0 to 36. 99 copy of DNA/µg DNA. The viral load in Dendronereis is comparable with that of naturally infected and at carrier state P.monodon from the same pond. Infected tissue observed were gills and mucosal lining of the intestine. The results implies that Dendronereis spp is a potential carrier of WSSV in the shrimp pond.
|Publication status||Published - 2011|
|Event||Symposium on Coastal Resources Management and Development, Semarang, Indonesia, 29-30 November 2011 - |
Duration: 29 Nov 2011 → 30 Nov 2011
|Conference||Symposium on Coastal Resources Management and Development, Semarang, Indonesia, 29-30 November 2011|
|Period||29/11/11 → 30/11/11|