Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities

Matthew Carson, Julia K. Keppler, Gilles Brackman, Daniel Dawood, Marta Vandrovcova, Karim Fawzy El-Sayed, Tom Coenye, Karin Schwarz, Susan A. Clarke, Andre G. Skirtach, Timothy E.L. Douglas

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)


Polyphenols are known for their antimicrobial activity, whilst both polyphenols and the globular protein β-lactoglobulin (bLG) are suggested to have antioxidant properties and promote cell proliferation. These are potentially useful properties for a tissue-engineered construct, though it is unknown if they are retained when both compounds are used in combination. In this study, a range of different microbes and an osteoblast-like cell line (human fetal osteoblast, hFOB) were used to assess the combined effect of: (1) green tea extract (GTE), rich in the polyphenol epigallocatechin gallate (EGCG), and (2) whey protein isolate (WPI), rich in bLG. It was shown that approximately 20-48% of the EGCG in GTE reacted with WPI. GTE inhibited the growth of Gram-positive bacteria, an effect which was potentiated by the addition of WPI. GTE alone also significantly inhibited the growth of hFOB cells after 1, 4, and 7 days of culture. Alternatively, WPI significantly promoted hFOB cell growth in the absence of GTE and attenuated the effect of GTE at low concentrations (64 μg/mL) after 4 and 7 days. Low concentrations of WPI (50 μg/mL) also promoted the expression of the early osteogenic marker alkaline phosphatase (ALP) by hFOB cells, whereas GTE inhibited ALP activity. Therefore, the antioxidant effects of GTE can be boosted by WPI, but GTE is not suitable to be used as part of a tissue-engineered construct due to its cytotoxic effects which negate any positive effect WPI has on cell proliferation.

Original languageEnglish
Pages (from-to)106-117
Number of pages12
JournalCells Tissues Organs
Issue number1-2
Publication statusPublished - May 2019
Externally publishedYes


  • Antibacterial properties
  • Antioxidant
  • Osteoblast
  • Polyphenol
  • Whey protein isolate


Dive into the research topics of 'Whey Protein Complexes with Green Tea Polyphenols: Antimicrobial, Osteoblast-Stimulatory, and Antioxidant Activities'. Together they form a unique fingerprint.

Cite this