Weight loss moderately affects the mixed meal challenge response of the plasma metabolome and transcriptome of peripheral blood mononuclear cells in abdominally obese subjects

Parastoo Fazelzadeh, Roland W.J. Hangelbroek, Peter J. Joris, Casper G. Schalkwijk, Diederik Esser, Lydia Afman, Thomas Hankemeier, Doris M. Jacobs, Velitchka V. Mihaleva, Sander Kersten, John van Duynhoven, Mark V. Boekschoten

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Introduction: The response to dietary challenges has been proposed as a more accurate measure of metabolic health than static measurements performed in the fasted state. This has prompted many groups to explore the potential of dietary challenge tests for assessment of diet and lifestyle induced shifts in metabolic phenotype. Objectives: We examined whether the response to a mixed-meal challenge could provide a readout for a weight loss (WL)-induced phenotype shift in abdominally obese male subjects. The underlying assumption of a mixed meal challenge is that it triggers all aspects of phenotypic flexibility and provokes a more prolonged insulin response, possibly allowing for better differentiation between individuals. Methods: Abdominally obese men (n = 29, BMI = 30.3 ± 2.4 kg/m2) received a mixed-meal challenge prior to and after an 8-week WL or no-WL control intervention. Lean subjects (n = 15, BMI = 23.0 ± 2.0 kg/m2) only received the mixed meal challenge at baseline to have a benchmark for WL-induced phenotype shifts. Results: Levels of several plasma metabolites were significantly different between lean and abdominally obese at baseline as well as during postprandial metabolic responses. Genes related to oxidative phosphorylation in peripheral blood mononuclear cells (PBMCs) were expressed at higher levels in abdominally obese subjects as compared to lean subjects at fasting, which was partially reverted after WL. The impact of WL on the postprandial response was modest, both at the metabolic and gene expression level in PBMCs. Conclusion: We conclude that mixed-meal challenges are not necessarily superior to measurements in the fasted state to assess metabolic health. Furthermore, the mechanisms accounting for the observed differences between lean and abdominally obese in the fasted state are different from those underlying the dissimilarity observed during the postprandial response.
Original languageEnglish
Article number46
JournalMetabolomics
Volume14
Issue number4
DOIs
Publication statusPublished - 1 Apr 2018

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Metabolome
Transcriptome
Meals
Weight Loss
Blood Cells
Blood
Plasmas
Phenotype
Health
Benchmarking
Oxidative Phosphorylation
Nutrition
Metabolites
Gene expression
Life Style
Fasting
Genes
Insulin
Diet
Gene Expression

Keywords

  • Metabolic health
  • Mixed-meal challenge
  • Phenotype shift

Cite this

@article{fb745917527a4679a39c586159a9c135,
title = "Weight loss moderately affects the mixed meal challenge response of the plasma metabolome and transcriptome of peripheral blood mononuclear cells in abdominally obese subjects",
abstract = "Introduction: The response to dietary challenges has been proposed as a more accurate measure of metabolic health than static measurements performed in the fasted state. This has prompted many groups to explore the potential of dietary challenge tests for assessment of diet and lifestyle induced shifts in metabolic phenotype. Objectives: We examined whether the response to a mixed-meal challenge could provide a readout for a weight loss (WL)-induced phenotype shift in abdominally obese male subjects. The underlying assumption of a mixed meal challenge is that it triggers all aspects of phenotypic flexibility and provokes a more prolonged insulin response, possibly allowing for better differentiation between individuals. Methods: Abdominally obese men (n = 29, BMI = 30.3 ± 2.4 kg/m2) received a mixed-meal challenge prior to and after an 8-week WL or no-WL control intervention. Lean subjects (n = 15, BMI = 23.0 ± 2.0 kg/m2) only received the mixed meal challenge at baseline to have a benchmark for WL-induced phenotype shifts. Results: Levels of several plasma metabolites were significantly different between lean and abdominally obese at baseline as well as during postprandial metabolic responses. Genes related to oxidative phosphorylation in peripheral blood mononuclear cells (PBMCs) were expressed at higher levels in abdominally obese subjects as compared to lean subjects at fasting, which was partially reverted after WL. The impact of WL on the postprandial response was modest, both at the metabolic and gene expression level in PBMCs. Conclusion: We conclude that mixed-meal challenges are not necessarily superior to measurements in the fasted state to assess metabolic health. Furthermore, the mechanisms accounting for the observed differences between lean and abdominally obese in the fasted state are different from those underlying the dissimilarity observed during the postprandial response.",
keywords = "Metabolic health, Mixed-meal challenge, Phenotype shift",
author = "Parastoo Fazelzadeh and Hangelbroek, {Roland W.J.} and Joris, {Peter J.} and Schalkwijk, {Casper G.} and Diederik Esser and Lydia Afman and Thomas Hankemeier and Jacobs, {Doris M.} and Mihaleva, {Velitchka V.} and Sander Kersten and {van Duynhoven}, John and Boekschoten, {Mark V.}",
year = "2018",
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doi = "10.1007/s11306-018-1328-x",
language = "English",
volume = "14",
journal = "Metabolomics",
issn = "1573-3882",
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Weight loss moderately affects the mixed meal challenge response of the plasma metabolome and transcriptome of peripheral blood mononuclear cells in abdominally obese subjects. / Fazelzadeh, Parastoo; Hangelbroek, Roland W.J.; Joris, Peter J.; Schalkwijk, Casper G.; Esser, Diederik; Afman, Lydia; Hankemeier, Thomas; Jacobs, Doris M.; Mihaleva, Velitchka V.; Kersten, Sander; van Duynhoven, John; Boekschoten, Mark V.

In: Metabolomics, Vol. 14, No. 4, 46, 01.04.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Weight loss moderately affects the mixed meal challenge response of the plasma metabolome and transcriptome of peripheral blood mononuclear cells in abdominally obese subjects

AU - Fazelzadeh, Parastoo

AU - Hangelbroek, Roland W.J.

AU - Joris, Peter J.

AU - Schalkwijk, Casper G.

AU - Esser, Diederik

AU - Afman, Lydia

AU - Hankemeier, Thomas

AU - Jacobs, Doris M.

AU - Mihaleva, Velitchka V.

AU - Kersten, Sander

AU - van Duynhoven, John

AU - Boekschoten, Mark V.

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Introduction: The response to dietary challenges has been proposed as a more accurate measure of metabolic health than static measurements performed in the fasted state. This has prompted many groups to explore the potential of dietary challenge tests for assessment of diet and lifestyle induced shifts in metabolic phenotype. Objectives: We examined whether the response to a mixed-meal challenge could provide a readout for a weight loss (WL)-induced phenotype shift in abdominally obese male subjects. The underlying assumption of a mixed meal challenge is that it triggers all aspects of phenotypic flexibility and provokes a more prolonged insulin response, possibly allowing for better differentiation between individuals. Methods: Abdominally obese men (n = 29, BMI = 30.3 ± 2.4 kg/m2) received a mixed-meal challenge prior to and after an 8-week WL or no-WL control intervention. Lean subjects (n = 15, BMI = 23.0 ± 2.0 kg/m2) only received the mixed meal challenge at baseline to have a benchmark for WL-induced phenotype shifts. Results: Levels of several plasma metabolites were significantly different between lean and abdominally obese at baseline as well as during postprandial metabolic responses. Genes related to oxidative phosphorylation in peripheral blood mononuclear cells (PBMCs) were expressed at higher levels in abdominally obese subjects as compared to lean subjects at fasting, which was partially reverted after WL. The impact of WL on the postprandial response was modest, both at the metabolic and gene expression level in PBMCs. Conclusion: We conclude that mixed-meal challenges are not necessarily superior to measurements in the fasted state to assess metabolic health. Furthermore, the mechanisms accounting for the observed differences between lean and abdominally obese in the fasted state are different from those underlying the dissimilarity observed during the postprandial response.

AB - Introduction: The response to dietary challenges has been proposed as a more accurate measure of metabolic health than static measurements performed in the fasted state. This has prompted many groups to explore the potential of dietary challenge tests for assessment of diet and lifestyle induced shifts in metabolic phenotype. Objectives: We examined whether the response to a mixed-meal challenge could provide a readout for a weight loss (WL)-induced phenotype shift in abdominally obese male subjects. The underlying assumption of a mixed meal challenge is that it triggers all aspects of phenotypic flexibility and provokes a more prolonged insulin response, possibly allowing for better differentiation between individuals. Methods: Abdominally obese men (n = 29, BMI = 30.3 ± 2.4 kg/m2) received a mixed-meal challenge prior to and after an 8-week WL or no-WL control intervention. Lean subjects (n = 15, BMI = 23.0 ± 2.0 kg/m2) only received the mixed meal challenge at baseline to have a benchmark for WL-induced phenotype shifts. Results: Levels of several plasma metabolites were significantly different between lean and abdominally obese at baseline as well as during postprandial metabolic responses. Genes related to oxidative phosphorylation in peripheral blood mononuclear cells (PBMCs) were expressed at higher levels in abdominally obese subjects as compared to lean subjects at fasting, which was partially reverted after WL. The impact of WL on the postprandial response was modest, both at the metabolic and gene expression level in PBMCs. Conclusion: We conclude that mixed-meal challenges are not necessarily superior to measurements in the fasted state to assess metabolic health. Furthermore, the mechanisms accounting for the observed differences between lean and abdominally obese in the fasted state are different from those underlying the dissimilarity observed during the postprandial response.

KW - Metabolic health

KW - Mixed-meal challenge

KW - Phenotype shift

U2 - 10.1007/s11306-018-1328-x

DO - 10.1007/s11306-018-1328-x

M3 - Article

VL - 14

JO - Metabolomics

JF - Metabolomics

SN - 1573-3882

IS - 4

M1 - 46

ER -