Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding

G.A. de Groot, Stefan Geisen, E.R.J. Wubs, I. Laros, Liz Meulenbroek, A.T. Kuiters, P.A. Slim

Research output: Contribution to conferenceAbstractAcademic

Abstract

Understanding variation in airborne biological dispersal is relevant not only to understand biogeographical patterns, but also because various biological particles may be allergenic or pathogenic to plants or animals (including humans). Weather conditions will influence both the release and spread of biological propagules in the air, and thus the composition of the airborne community (the ‘aerobiome’). Molecular techniques are increasingly used to study airborne dispersal of bacteria and fungi, although often including a culturing step. Other life forms, mostly plants, have mainly been studied by analysing samples under the microscope. This limits the spatiotemporal scope and the ability to directly link the dispersal of flora and fauna to that of microbes.
We used DNA metabarcoding based on three genetic markers to study the entire aerobiome in direct DNA extracts from air samples taken over a continuous period of 21 days at two different sites on the Wageningen Campus. We observed a large diversity of microbes (bacteria, fungi and protists) as well as plants. Using multivariate and network analyses, we studied relations between the presence of taxa from different branches of the tree of life, and explored how community shifts related to meteorological parameters and life history traits.
We show that communities were dominated by spore forming fungi and protists, many of those resembling (plant) pathogens. Relations with weather conditions varied among taxa. Oomycetes for example, predominantly containing (plant) pathogens, occurred in much higher relative abundances at low air humidity, while many fungi and plants were still abundant on very rainy days. These types of observations may help to explore potential effects of climatic changes on species distributions and the spread of pests and diseases.

Conference

ConferenceAnnual meeting of British Ecological Society (BES)
CountryUnited Kingdom
CityLiverpool
Period11/12/1614/12/16

Fingerprint

weather
microorganisms
fungi
DNA
plant pathogens
air
species dispersal
meteorological parameters
Oomycetes
bacteria
plant architecture
microscopes
humidity
biogeography
spores
flora
life history
climate change
fauna
pests

Cite this

de Groot, G. A., Geisen, S., Wubs, E. R. J., Laros, I., Meulenbroek, L., Kuiters, A. T., & Slim, P. A. (2016). Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding. Abstract from Annual meeting of British Ecological Society (BES), Liverpool, United Kingdom.
de Groot, G.A. ; Geisen, Stefan ; Wubs, E.R.J. ; Laros, I. ; Meulenbroek, Liz ; Kuiters, A.T. ; Slim, P.A. / Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding. Abstract from Annual meeting of British Ecological Society (BES), Liverpool, United Kingdom.
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title = "Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding",
abstract = "Understanding variation in airborne biological dispersal is relevant not only to understand biogeographical patterns, but also because various biological particles may be allergenic or pathogenic to plants or animals (including humans). Weather conditions will influence both the release and spread of biological propagules in the air, and thus the composition of the airborne community (the ‘aerobiome’). Molecular techniques are increasingly used to study airborne dispersal of bacteria and fungi, although often including a culturing step. Other life forms, mostly plants, have mainly been studied by analysing samples under the microscope. This limits the spatiotemporal scope and the ability to directly link the dispersal of flora and fauna to that of microbes. We used DNA metabarcoding based on three genetic markers to study the entire aerobiome in direct DNA extracts from air samples taken over a continuous period of 21 days at two different sites on the Wageningen Campus. We observed a large diversity of microbes (bacteria, fungi and protists) as well as plants. Using multivariate and network analyses, we studied relations between the presence of taxa from different branches of the tree of life, and explored how community shifts related to meteorological parameters and life history traits. We show that communities were dominated by spore forming fungi and protists, many of those resembling (plant) pathogens. Relations with weather conditions varied among taxa. Oomycetes for example, predominantly containing (plant) pathogens, occurred in much higher relative abundances at low air humidity, while many fungi and plants were still abundant on very rainy days. These types of observations may help to explore potential effects of climatic changes on species distributions and the spread of pests and diseases.",
author = "{de Groot}, G.A. and Stefan Geisen and E.R.J. Wubs and I. Laros and Liz Meulenbroek and A.T. Kuiters and P.A. Slim",
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note = "Annual meeting of British Ecological Society (BES) ; Conference date: 11-12-2016 Through 14-12-2016",

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de Groot, GA, Geisen, S, Wubs, ERJ, Laros, I, Meulenbroek, L, Kuiters, AT & Slim, PA 2016, 'Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding' Annual meeting of British Ecological Society (BES), Liverpool, United Kingdom, 11/12/16 - 14/12/16, .

Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding. / de Groot, G.A.; Geisen, Stefan; Wubs, E.R.J.; Laros, I.; Meulenbroek, Liz; Kuiters, A.T.; Slim, P.A.

2016. Abstract from Annual meeting of British Ecological Society (BES), Liverpool, United Kingdom.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding

AU - de Groot, G.A.

AU - Geisen, Stefan

AU - Wubs, E.R.J.

AU - Laros, I.

AU - Meulenbroek, Liz

AU - Kuiters, A.T.

AU - Slim, P.A.

PY - 2016/12/13

Y1 - 2016/12/13

N2 - Understanding variation in airborne biological dispersal is relevant not only to understand biogeographical patterns, but also because various biological particles may be allergenic or pathogenic to plants or animals (including humans). Weather conditions will influence both the release and spread of biological propagules in the air, and thus the composition of the airborne community (the ‘aerobiome’). Molecular techniques are increasingly used to study airborne dispersal of bacteria and fungi, although often including a culturing step. Other life forms, mostly plants, have mainly been studied by analysing samples under the microscope. This limits the spatiotemporal scope and the ability to directly link the dispersal of flora and fauna to that of microbes. We used DNA metabarcoding based on three genetic markers to study the entire aerobiome in direct DNA extracts from air samples taken over a continuous period of 21 days at two different sites on the Wageningen Campus. We observed a large diversity of microbes (bacteria, fungi and protists) as well as plants. Using multivariate and network analyses, we studied relations between the presence of taxa from different branches of the tree of life, and explored how community shifts related to meteorological parameters and life history traits. We show that communities were dominated by spore forming fungi and protists, many of those resembling (plant) pathogens. Relations with weather conditions varied among taxa. Oomycetes for example, predominantly containing (plant) pathogens, occurred in much higher relative abundances at low air humidity, while many fungi and plants were still abundant on very rainy days. These types of observations may help to explore potential effects of climatic changes on species distributions and the spread of pests and diseases.

AB - Understanding variation in airborne biological dispersal is relevant not only to understand biogeographical patterns, but also because various biological particles may be allergenic or pathogenic to plants or animals (including humans). Weather conditions will influence both the release and spread of biological propagules in the air, and thus the composition of the airborne community (the ‘aerobiome’). Molecular techniques are increasingly used to study airborne dispersal of bacteria and fungi, although often including a culturing step. Other life forms, mostly plants, have mainly been studied by analysing samples under the microscope. This limits the spatiotemporal scope and the ability to directly link the dispersal of flora and fauna to that of microbes. We used DNA metabarcoding based on three genetic markers to study the entire aerobiome in direct DNA extracts from air samples taken over a continuous period of 21 days at two different sites on the Wageningen Campus. We observed a large diversity of microbes (bacteria, fungi and protists) as well as plants. Using multivariate and network analyses, we studied relations between the presence of taxa from different branches of the tree of life, and explored how community shifts related to meteorological parameters and life history traits. We show that communities were dominated by spore forming fungi and protists, many of those resembling (plant) pathogens. Relations with weather conditions varied among taxa. Oomycetes for example, predominantly containing (plant) pathogens, occurred in much higher relative abundances at low air humidity, while many fungi and plants were still abundant on very rainy days. These types of observations may help to explore potential effects of climatic changes on species distributions and the spread of pests and diseases.

M3 - Abstract

ER -

de Groot GA, Geisen S, Wubs ERJ, Laros I, Meulenbroek L, Kuiters AT et al. Weather-dependent community shiftsin the aerobiome: measuring airborne dispersal of plants, mesofauna and microbes simultaneously via DNA metabarcoding. 2016. Abstract from Annual meeting of British Ecological Society (BES), Liverpool, United Kingdom.