Water flow induced transport of Pseudomonas fluorescens cells through soil columns as affected by inoculant treatment

W.E. Hekman, C.E. Heijnen, J.T. Trevors, J.D. van Elsas

    Research output: Contribution to journalArticleAcademicpeer-review

    25 Citations (Scopus)

    Abstract

    Water flow induced transport of Pseudomonas fluorescens cells through soil columns was measured as affected by the inoculant treatment. Bacterial cells were introduced into the topsoil of columns, either encapsulated in alginate beads of different types or mixed with bentonite clay in concentrations ranging from 0.5 to 5.0% (w/v). Survival of bacterial cells was improved with the use of alginate or bentonite. Transport, as determined by destructive sampling of the columns, was reduced with the use of alginate encapsulation. Drying of the beads had no influence on transport. The presence of bentonite in the topsoil, either pre-mixed through the soil, or applied as a slurry together with the bacteria, also reduced transport, except when 0.5% was pre-mixed through the soil. P. fluorescens cells encapsulated in alginate beads prepared with water and supplemented with skim milk powder and bentonite showed the best survival during the time of the experiment and the most reduced transport compared to the control. Therefore, cells encapsulated in this way are suitable, due to their optimal survival and reduced spread, for use in a field experiment with genetically manipulated bacteria.
    Original languageEnglish
    Pages (from-to)313-326
    JournalFEMS microbiology ecology
    Volume13
    Issue number4
    DOIs
    Publication statusPublished - 1994

    Fingerprint

    Bentonite
    Pseudomonas fluorescens
    alginate
    soil column
    bentonite
    water flow
    Soil
    Water
    topsoil
    Bacteria
    bacterium
    encapsulation
    Powders
    milk
    slurry
    Cell Survival
    Milk
    soil
    clay
    alginic acid

    Cite this

    Hekman, W.E. ; Heijnen, C.E. ; Trevors, J.T. ; van Elsas, J.D. / Water flow induced transport of Pseudomonas fluorescens cells through soil columns as affected by inoculant treatment. In: FEMS microbiology ecology. 1994 ; Vol. 13, No. 4. pp. 313-326.
    @article{a34445f1944a48aa802da57a59f5122e,
    title = "Water flow induced transport of Pseudomonas fluorescens cells through soil columns as affected by inoculant treatment",
    abstract = "Water flow induced transport of Pseudomonas fluorescens cells through soil columns was measured as affected by the inoculant treatment. Bacterial cells were introduced into the topsoil of columns, either encapsulated in alginate beads of different types or mixed with bentonite clay in concentrations ranging from 0.5 to 5.0{\%} (w/v). Survival of bacterial cells was improved with the use of alginate or bentonite. Transport, as determined by destructive sampling of the columns, was reduced with the use of alginate encapsulation. Drying of the beads had no influence on transport. The presence of bentonite in the topsoil, either pre-mixed through the soil, or applied as a slurry together with the bacteria, also reduced transport, except when 0.5{\%} was pre-mixed through the soil. P. fluorescens cells encapsulated in alginate beads prepared with water and supplemented with skim milk powder and bentonite showed the best survival during the time of the experiment and the most reduced transport compared to the control. Therefore, cells encapsulated in this way are suitable, due to their optimal survival and reduced spread, for use in a field experiment with genetically manipulated bacteria.",
    author = "W.E. Hekman and C.E. Heijnen and J.T. Trevors and {van Elsas}, J.D.",
    year = "1994",
    doi = "10.1016/0168-6496(94)90069-8",
    language = "English",
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    Water flow induced transport of Pseudomonas fluorescens cells through soil columns as affected by inoculant treatment. / Hekman, W.E.; Heijnen, C.E.; Trevors, J.T.; van Elsas, J.D.

    In: FEMS microbiology ecology, Vol. 13, No. 4, 1994, p. 313-326.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Water flow induced transport of Pseudomonas fluorescens cells through soil columns as affected by inoculant treatment

    AU - Hekman, W.E.

    AU - Heijnen, C.E.

    AU - Trevors, J.T.

    AU - van Elsas, J.D.

    PY - 1994

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    AB - Water flow induced transport of Pseudomonas fluorescens cells through soil columns was measured as affected by the inoculant treatment. Bacterial cells were introduced into the topsoil of columns, either encapsulated in alginate beads of different types or mixed with bentonite clay in concentrations ranging from 0.5 to 5.0% (w/v). Survival of bacterial cells was improved with the use of alginate or bentonite. Transport, as determined by destructive sampling of the columns, was reduced with the use of alginate encapsulation. Drying of the beads had no influence on transport. The presence of bentonite in the topsoil, either pre-mixed through the soil, or applied as a slurry together with the bacteria, also reduced transport, except when 0.5% was pre-mixed through the soil. P. fluorescens cells encapsulated in alginate beads prepared with water and supplemented with skim milk powder and bentonite showed the best survival during the time of the experiment and the most reduced transport compared to the control. Therefore, cells encapsulated in this way are suitable, due to their optimal survival and reduced spread, for use in a field experiment with genetically manipulated bacteria.

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