Vitamin A equivalency of ß-carotene in healthy adults: limitation of the extrinsic dual-isotope dilution technique to measure matrix effect

C.A. Bouwman, C.E. West, R.B. van Breemen, D. Zhu, E. Siebelink, P. Versloot, P.J.M. Hulshof, M. van Lieshout, F.G.M. Russel, G. Schaafsma, T.H.J. Naber

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Abstract

Data on the vitamin A equivalency of ß-carotene in food are inconsistent. We quantified the vitamin A equivalency (¿g) of ß-carotene in two diets using the dual-isotope dilution technique and the oral¿faecal balance technique. A diet-controlled, cross-over intervention study was conducted in twenty-four healthy adults. Each subject followed two diets for 3 weeks each: a diet containing vegetables low in ß-carotene with supplemental ß-carotene in salad dressing oil ('oil diet') and a diet containing vegetables and fruits high in ß-carotene ('mixed diet'). During all 6 weeks, each subject daily consumed a mean of 55 (sd 0·5) ¿g [13C10]ß-carotene and 55 (sd 0·5) ¿g [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of ß-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum and from apparent absorption by oral-faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]ß-carotene in oil of 3·4 ¿g (95 % CI 2·8, 3·9), thus the bio-efficacy of the ß-carotene in oil was 28 % in the presence of both diets. However, data from oral-faecal balance estimated vitamin A equivalency as 6:1 ¿g (95 % CI 4, 7) for ß-carotene in the 'oil diet'. ß-Carotene in the 'oil diet' had 2·9-fold higher vitamin A equivalency than ß-carotene in the 'mixed diet'. In conclusion, this extrinsic labelling technique cannot measure effects of mixed vegetables and fruits matrices, but can measure precisely the vitamin A equivalency of the ß-carotene in oil capsules
Original languageEnglish
Pages (from-to)1837-1845
JournalBritish Journal of Nutrition
Volume101
Issue number12
DOIs
Publication statusPublished - 2009

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Indicator Dilution Techniques
Carotenoids
Vitamin A
Isotopes
Diet
Oils
Vegetables
Capsules
Fruit
Bandages
Cross-Over Studies

Keywords

  • mass-spectrometry
  • leafy vegetables
  • dark-green
  • bioavailability
  • retinol
  • humans
  • bioconversion
  • serum
  • consumption
  • spinach

Cite this

Bouwman, C.A. ; West, C.E. ; van Breemen, R.B. ; Zhu, D. ; Siebelink, E. ; Versloot, P. ; Hulshof, P.J.M. ; van Lieshout, M. ; Russel, F.G.M. ; Schaafsma, G. ; Naber, T.H.J. / Vitamin A equivalency of ß-carotene in healthy adults: limitation of the extrinsic dual-isotope dilution technique to measure matrix effect. In: British Journal of Nutrition. 2009 ; Vol. 101, No. 12. pp. 1837-1845.
@article{6063d9053ff74e42bb51d93715933bec,
title = "Vitamin A equivalency of {\ss}-carotene in healthy adults: limitation of the extrinsic dual-isotope dilution technique to measure matrix effect",
abstract = "Data on the vitamin A equivalency of {\ss}-carotene in food are inconsistent. We quantified the vitamin A equivalency (¿g) of {\ss}-carotene in two diets using the dual-isotope dilution technique and the oral¿faecal balance technique. A diet-controlled, cross-over intervention study was conducted in twenty-four healthy adults. Each subject followed two diets for 3 weeks each: a diet containing vegetables low in {\ss}-carotene with supplemental {\ss}-carotene in salad dressing oil ('oil diet') and a diet containing vegetables and fruits high in {\ss}-carotene ('mixed diet'). During all 6 weeks, each subject daily consumed a mean of 55 (sd 0·5) ¿g [13C10]{\ss}-carotene and 55 (sd 0·5) ¿g [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of {\ss}-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum and from apparent absorption by oral-faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]{\ss}-carotene in oil of 3·4 ¿g (95 {\%} CI 2·8, 3·9), thus the bio-efficacy of the {\ss}-carotene in oil was 28 {\%} in the presence of both diets. However, data from oral-faecal balance estimated vitamin A equivalency as 6:1 ¿g (95 {\%} CI 4, 7) for {\ss}-carotene in the 'oil diet'. {\ss}-Carotene in the 'oil diet' had 2·9-fold higher vitamin A equivalency than {\ss}-carotene in the 'mixed diet'. In conclusion, this extrinsic labelling technique cannot measure effects of mixed vegetables and fruits matrices, but can measure precisely the vitamin A equivalency of the {\ss}-carotene in oil capsules",
keywords = "mass-spectrometry, leafy vegetables, dark-green, bioavailability, retinol, humans, bioconversion, serum, consumption, spinach",
author = "C.A. Bouwman and C.E. West and {van Breemen}, R.B. and D. Zhu and E. Siebelink and P. Versloot and P.J.M. Hulshof and {van Lieshout}, M. and F.G.M. Russel and G. Schaafsma and T.H.J. Naber",
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Vitamin A equivalency of ß-carotene in healthy adults: limitation of the extrinsic dual-isotope dilution technique to measure matrix effect. / Bouwman, C.A.; West, C.E.; van Breemen, R.B.; Zhu, D.; Siebelink, E.; Versloot, P.; Hulshof, P.J.M.; van Lieshout, M.; Russel, F.G.M.; Schaafsma, G.; Naber, T.H.J.

In: British Journal of Nutrition, Vol. 101, No. 12, 2009, p. 1837-1845.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Vitamin A equivalency of ß-carotene in healthy adults: limitation of the extrinsic dual-isotope dilution technique to measure matrix effect

AU - Bouwman, C.A.

AU - West, C.E.

AU - van Breemen, R.B.

AU - Zhu, D.

AU - Siebelink, E.

AU - Versloot, P.

AU - Hulshof, P.J.M.

AU - van Lieshout, M.

AU - Russel, F.G.M.

AU - Schaafsma, G.

AU - Naber, T.H.J.

N1 - Online first

PY - 2009

Y1 - 2009

N2 - Data on the vitamin A equivalency of ß-carotene in food are inconsistent. We quantified the vitamin A equivalency (¿g) of ß-carotene in two diets using the dual-isotope dilution technique and the oral¿faecal balance technique. A diet-controlled, cross-over intervention study was conducted in twenty-four healthy adults. Each subject followed two diets for 3 weeks each: a diet containing vegetables low in ß-carotene with supplemental ß-carotene in salad dressing oil ('oil diet') and a diet containing vegetables and fruits high in ß-carotene ('mixed diet'). During all 6 weeks, each subject daily consumed a mean of 55 (sd 0·5) ¿g [13C10]ß-carotene and 55 (sd 0·5) ¿g [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of ß-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum and from apparent absorption by oral-faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]ß-carotene in oil of 3·4 ¿g (95 % CI 2·8, 3·9), thus the bio-efficacy of the ß-carotene in oil was 28 % in the presence of both diets. However, data from oral-faecal balance estimated vitamin A equivalency as 6:1 ¿g (95 % CI 4, 7) for ß-carotene in the 'oil diet'. ß-Carotene in the 'oil diet' had 2·9-fold higher vitamin A equivalency than ß-carotene in the 'mixed diet'. In conclusion, this extrinsic labelling technique cannot measure effects of mixed vegetables and fruits matrices, but can measure precisely the vitamin A equivalency of the ß-carotene in oil capsules

AB - Data on the vitamin A equivalency of ß-carotene in food are inconsistent. We quantified the vitamin A equivalency (¿g) of ß-carotene in two diets using the dual-isotope dilution technique and the oral¿faecal balance technique. A diet-controlled, cross-over intervention study was conducted in twenty-four healthy adults. Each subject followed two diets for 3 weeks each: a diet containing vegetables low in ß-carotene with supplemental ß-carotene in salad dressing oil ('oil diet') and a diet containing vegetables and fruits high in ß-carotene ('mixed diet'). During all 6 weeks, each subject daily consumed a mean of 55 (sd 0·5) ¿g [13C10]ß-carotene and 55 (sd 0·5) ¿g [13C10]retinyl palmitate in oil capsules. The vitamin A equivalency of ß-carotene was calculated as the dose-corrected ratio of [13C5]retinol to [13C10]retinol in serum and from apparent absorption by oral-faecal balance. Isotopic data quantified a vitamin A equivalency of [13C10]ß-carotene in oil of 3·4 ¿g (95 % CI 2·8, 3·9), thus the bio-efficacy of the ß-carotene in oil was 28 % in the presence of both diets. However, data from oral-faecal balance estimated vitamin A equivalency as 6:1 ¿g (95 % CI 4, 7) for ß-carotene in the 'oil diet'. ß-Carotene in the 'oil diet' had 2·9-fold higher vitamin A equivalency than ß-carotene in the 'mixed diet'. In conclusion, this extrinsic labelling technique cannot measure effects of mixed vegetables and fruits matrices, but can measure precisely the vitamin A equivalency of the ß-carotene in oil capsules

KW - mass-spectrometry

KW - leafy vegetables

KW - dark-green

KW - bioavailability

KW - retinol

KW - humans

KW - bioconversion

KW - serum

KW - consumption

KW - spinach

U2 - 10.1017/S0007114508131762

DO - 10.1017/S0007114508131762

M3 - Article

VL - 101

SP - 1837

EP - 1845

JO - The British journal of nutrition

JF - The British journal of nutrition

SN - 0007-1145

IS - 12

ER -