Visualisation of biopolymer mixtures using confocal scanning laser microscopy (CSLM) and covalent labelling techniques

F. van de Velde; F. Weinbreck; M.W. Edelman; E. van der Linden; R.H. Tromp

doi:10.1016/S0927-7765(03)00135-8

Visualisation of biopolymer mixtures using confocal scanning laser microscopy (CSLM) and covalent labelling techniques

F. van de Velde, F. Weinbreck, M.W. Edelman, E. van der Linden, R.H. Tromp

Research output: Contribution to journal › Article › Academic › peer-review

97 Citations (Scopus)

Abstract

Confocal scanning laser microscopy (CSLM) has been used to study the behaviour of mixtures of proteins, gelatine, whey proteins and ß-lactoglobulin, and polysaccharides, dextran, gellan gum, carrageenan, gum Arabic, and starch. CSLM proved to be a suitable technique to visualise the microstructure of these (phase separated) mixtures in two and three-dimensional images. Contrast through fluorescence is obtained either by covalent labelling (polysaccharides and proteins) or non-covalent labelling (proteins and starch). Double and triple labelling allows the visualisation of individual components in a complex mixture of biopolymers.

Original language	English
Pages (from-to)	159-168
Journal	Colloids and Surfaces. B: Biointerfaces
Volume	31
Issue number	1-4
DOIs	https://doi.org/10.1016/S0927-7765(03)00135-8
Publication status	Published - 2003

Keywords

phase-separation
light-microscopy
whey
proteins

Access to Document

10.1016/S0927-7765(03)00135-8

Cite this

@article{7598b5fb349549dd9403b79e151a54be,

title = "Visualisation of biopolymer mixtures using confocal scanning laser microscopy (CSLM) and covalent labelling techniques",

abstract = "Confocal scanning laser microscopy (CSLM) has been used to study the behaviour of mixtures of proteins, gelatine, whey proteins and {\ss}-lactoglobulin, and polysaccharides, dextran, gellan gum, carrageenan, gum Arabic, and starch. CSLM proved to be a suitable technique to visualise the microstructure of these (phase separated) mixtures in two and three-dimensional images. Contrast through fluorescence is obtained either by covalent labelling (polysaccharides and proteins) or non-covalent labelling (proteins and starch). Double and triple labelling allows the visualisation of individual components in a complex mixture of biopolymers.",

keywords = "phase-separation, light-microscopy, whey, proteins",

author = "{van de Velde}, F. and F. Weinbreck and M.W. Edelman and {van der Linden}, E. and R.H. Tromp",

note = "F704",

year = "2003",

doi = "10.1016/S0927-7765(03)00135-8",

language = "English",

volume = "31",

pages = "159--168",

journal = "Colloids and Surfaces. B: Biointerfaces",

issn = "0927-7765",

publisher = "Elsevier",

number = "1-4",

}

TY - JOUR

T1 - Visualisation of biopolymer mixtures using confocal scanning laser microscopy (CSLM) and covalent labelling techniques

AU - van de Velde, F.

AU - Weinbreck, F.

AU - Edelman, M.W.

AU - van der Linden, E.

AU - Tromp, R.H.

N1 - F704

PY - 2003

Y1 - 2003

N2 - Confocal scanning laser microscopy (CSLM) has been used to study the behaviour of mixtures of proteins, gelatine, whey proteins and ß-lactoglobulin, and polysaccharides, dextran, gellan gum, carrageenan, gum Arabic, and starch. CSLM proved to be a suitable technique to visualise the microstructure of these (phase separated) mixtures in two and three-dimensional images. Contrast through fluorescence is obtained either by covalent labelling (polysaccharides and proteins) or non-covalent labelling (proteins and starch). Double and triple labelling allows the visualisation of individual components in a complex mixture of biopolymers.

AB - Confocal scanning laser microscopy (CSLM) has been used to study the behaviour of mixtures of proteins, gelatine, whey proteins and ß-lactoglobulin, and polysaccharides, dextran, gellan gum, carrageenan, gum Arabic, and starch. CSLM proved to be a suitable technique to visualise the microstructure of these (phase separated) mixtures in two and three-dimensional images. Contrast through fluorescence is obtained either by covalent labelling (polysaccharides and proteins) or non-covalent labelling (proteins and starch). Double and triple labelling allows the visualisation of individual components in a complex mixture of biopolymers.

KW - phase-separation

KW - light-microscopy

KW - whey

KW - proteins

U2 - 10.1016/S0927-7765(03)00135-8

DO - 10.1016/S0927-7765(03)00135-8

M3 - Article

VL - 31

SP - 159

EP - 168

JO - Colloids and Surfaces. B: Biointerfaces

JF - Colloids and Surfaces. B: Biointerfaces

SN - 0927-7765

IS - 1-4

ER -

Visualisation of biopolymer mixtures using confocal scanning laser microscopy (CSLM) and covalent labelling techniques

Abstract

Keywords

Access to Document

Fingerprint

Cite this