@inbook{88afef86ab9d4345b3f0fbd977146570,
title = "Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum",
abstract = "Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.",
keywords = "Agroinfiltration, ATTA, Ave1, Effector, Hypersensitive response (HR), Tobacco, Tobacco rattle virus, Ve1, VIGS",
author = "Z. Zhang and B.P.H.J. Thomma",
year = "2014",
doi = "10.1007/978-1-62703-986-4_14",
language = "English",
isbn = "9781627039857",
volume = "1127",
series = "Methods in molecular biology",
publisher = "Humana Press",
pages = "173--181",
editor = "P. Birch and J.T. Jones and J.L.B. Bos",
booktitle = "Plant-Pathogen Interactions",
address = "United States",
}