Virus-induced gene silencing and Agrobacterium tumefaciens-mediated transient expression in Nicotiana tabacum

Z. Zhang, B.P.H.J. Thomma

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

9 Citations (Scopus)

Abstract

Virus-induced gene silencing (VIGS) is a rapid method for transient silencing of plant genes. In this chapter, we describe the methodology for Tobacco rattle virus (TRV)-based VIGS in Nicotiana tabacum. In combination with subsequent co-expression of the tomato immune receptor Ve1 and the corresponding Verticillium effector Ave1 through Agrobacterium tumefaciens-mediated transient transformation (agroinfiltration), we established a rapid system for assessing the requirement of candidate plant genes for Ve1-mediated immune signaling.
Original languageEnglish
Title of host publicationPlant-Pathogen Interactions
Subtitle of host publicationMethods and Protocols
EditorsP. Birch, J.T. Jones, J.L.B. Bos
Place of PublicationTotowa
PublisherHumana Press
Pages173-181
Volume1127
ISBN (Electronic)9781627039864
ISBN (Print)9781627039857
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in molecular biology
PublisherSpringer Verlag
ISSN (Print)1064-3745

Keywords

  • Agroinfiltration
  • ATTA
  • Ave1
  • Effector
  • Hypersensitive response (HR)
  • Tobacco
  • Tobacco rattle virus
  • Ve1
  • VIGS

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