Abstract
Some pigeon paramyxovirus type 1 (PPMV-1) strains exhibit low virulence in chickens, despite their fusion (F) protein's multi-basic cleavage site. To elucidate the molecular basis of the low pathogenicity of these strains, we constructed an infectious full-length cDNA clone of PPMV-1 strain AV324. This strain is non-virulent for chickens, although its F protein contains the typical virulence motif (RRKKRF117)-R-112. By using reverse genetics, we exchanged the F genes of AV324 and a virulent Newcastle disease virus (NDV) strain (Herts) and evaluated the recovered chimeric viruses for their pathogenicity in 1-day-old chickens and in embryonated eggs. Our results show that the F protein of AV324, and probably those of similar PPMV-1 strains, are functionally not different from those of virulent NDV strains and that the difference in pathogenicity must be determined by other factors.
Original language | English |
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Pages (from-to) | 2746-2750 |
Journal | Journal of General Virology |
Volume | 90 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2009 |
Keywords
- newcastle-disease virus
- complete nucleotide-sequence
- pathogenicity
- expression
- cleavage