Virulence diversity of Phakopsora pachyrhizi isolates from East Africa compared to a geographically diverse collection

H.M. Murithi, J.S. Haudenshield, F. Beed, G. Mahuku, M.H.A.J. Joosten, G.L. Hartman

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3 Citations (Scopus)

Abstract

Soybean rust, caused by the biotrophic pathogen Phakopsora pachyrhizi, is a highly destructive disease causing substantial yield losses in many soybean producing regions throughout the world. Knowledge about P. pachyrhizi virulence is needed to guide development and deployment of soybean germplasm with durable resistance against all pathogen populations. To assess the virulence diversity of P. pachyrhizi, 25 isolates from eight countries, including 17 isolates from Africa, were characterized on 11 soybean genotypes serving as differentials. All the isolates induced tan lesions with abundant sporulation on genotypes without any known resistance genes and on soybean genotypes with resistance genes Rpp4 and Rpp5b. The most durable gene was Rpp2, where 96% of the isolates induced reddish brown lesions with little or no sporulation. Of the African isolates tested, the South African isolate was the most virulent, whereas those from Kenya, Malawi, and some of the isolates from Tanzania had the lowest virulence. An Argentinian isolate was virulent on most host differentials, including two cultivars carrying multiple resistance genes. Ten distinct pathotypes were identified, four of which comprised the African isolates representing considerable P. pachyrhizi virulence. Soybean genotypes carrying Rpp1b, Rpp2, Rpp3, and Rpp5 resistance genes and cultivars Hyuuga and UG5 were observed to be resistant against most of the African isolates and therefore may be useful for soybean-breeding programs in Africa or elsewhere.

LanguageEnglish
Pages1194-1200
JournalPlant Disease
Volume101
Issue number7
DOIs
Publication statusPublished - 2017

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Phakopsora pachyrhizi
Eastern Africa
virulence
soybeans
genotype
sporulation
genes
soybean rust
Malawi
pathogens
pathotypes
cultivars
Tanzania
Kenya
germplasm
breeding

Cite this

Murithi, H.M. ; Haudenshield, J.S. ; Beed, F. ; Mahuku, G. ; Joosten, M.H.A.J. ; Hartman, G.L. / Virulence diversity of Phakopsora pachyrhizi isolates from East Africa compared to a geographically diverse collection. In: Plant Disease. 2017 ; Vol. 101, No. 7. pp. 1194-1200.
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abstract = "Soybean rust, caused by the biotrophic pathogen Phakopsora pachyrhizi, is a highly destructive disease causing substantial yield losses in many soybean producing regions throughout the world. Knowledge about P. pachyrhizi virulence is needed to guide development and deployment of soybean germplasm with durable resistance against all pathogen populations. To assess the virulence diversity of P. pachyrhizi, 25 isolates from eight countries, including 17 isolates from Africa, were characterized on 11 soybean genotypes serving as differentials. All the isolates induced tan lesions with abundant sporulation on genotypes without any known resistance genes and on soybean genotypes with resistance genes Rpp4 and Rpp5b. The most durable gene was Rpp2, where 96{\%} of the isolates induced reddish brown lesions with little or no sporulation. Of the African isolates tested, the South African isolate was the most virulent, whereas those from Kenya, Malawi, and some of the isolates from Tanzania had the lowest virulence. An Argentinian isolate was virulent on most host differentials, including two cultivars carrying multiple resistance genes. Ten distinct pathotypes were identified, four of which comprised the African isolates representing considerable P. pachyrhizi virulence. Soybean genotypes carrying Rpp1b, Rpp2, Rpp3, and Rpp5 resistance genes and cultivars Hyuuga and UG5 were observed to be resistant against most of the African isolates and therefore may be useful for soybean-breeding programs in Africa or elsewhere.",
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Virulence diversity of Phakopsora pachyrhizi isolates from East Africa compared to a geographically diverse collection. / Murithi, H.M.; Haudenshield, J.S.; Beed, F.; Mahuku, G.; Joosten, M.H.A.J.; Hartman, G.L.

In: Plant Disease, Vol. 101, No. 7, 2017, p. 1194-1200.

Research output: Contribution to journalArticleAcademicpeer-review

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