Virulence-associated gene profiling of Streptococcus suis isolates by PCR

L.M.G. Silva, C.G. Baums, T. Rehm, H.J. Wisselink, R. Goethe, P. Valentin-Weigand

    Research output: Contribution to journalArticleAcademicpeer-review

    118 Citations (Scopus)

    Abstract

    Definition of virulent Streptococcus suis strains is controversial. One successful approach for identification of virulent European strains is differentiation of capsular serotypes (or the corresponding cps types) and subsequent detection of virulence-associated factors, namely the extracellular factor (EF, epf), the muramidase-released protein (MRP, mrp) and the hemolysin suilysin (SLY, sly). In this work we present a novel multiplex PCR (MP-PCR) and an mrp variant PCR for identification and characterization of virulent S. suis strains. These new methods were used to identify association of disease with particular profiles of virulence-associated genes. The MP-PCR allowed identification of S. suis through detection of the housekeeping gene gdh, differentiation of four cps types (1, 2, 7 and 9), and detection of epf, mrp, sly and arcA (arginine deiminase from S. suis). Furthermore, this study describes the first PCR assay for differentiation of at least six mrp variants. Expression of the corresponding size variants of MRP was shown for four of the six mrp variants, but was undetectable for the two larger mrp variants in the particular strains investigated. The results of this study suggest that cps7 strains are associated with pneumonia and that variation of mrp is very pronounced among these strains. Gene profiles of invasive, pneumonia and carrier S. suis isolates by combination of PCR assays allowed differentiation of 24 different genotypes among cps1, 2, 7 and 9 strains. Forty-five percent of the invasive S. suis diseases investigated in this study were caused by only two of these genotypes, namely cps2/mrp+/epf+/sly+ and cps9/mrp*/epf¿/sly+. Thus, this study demonstrates for the first time a uniform profile of the particular virulence-associated genes for the vast majority of the investigated invasive cps9 strains.
    Original languageEnglish
    Pages (from-to)117-127
    JournalVeterinary Microbiology
    Volume115
    Issue number1-3
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Streptococcus suis
    Virulence
    virulence
    Polymerase Chain Reaction
    Genes
    genes
    Multiplex Polymerase Chain Reaction
    Pneumonia
    Genotype
    pneumonia
    Hemolysin Proteins
    Essential Genes
    arginine deiminase
    Virulence Factors
    Muramidase
    hemolysins
    genotype
    assays
    lysozyme
    serotypes

    Keywords

    • muramidase-released protein
    • germ-free pigs
    • arginine deiminase system
    • tonsillar specimens
    • type-2 strains
    • diseased pigs
    • serotype 7
    • identification
    • expression
    • suilysin

    Cite this

    Silva, L. M. G., Baums, C. G., Rehm, T., Wisselink, H. J., Goethe, R., & Valentin-Weigand, P. (2006). Virulence-associated gene profiling of Streptococcus suis isolates by PCR. Veterinary Microbiology, 115(1-3), 117-127. https://doi.org/10.1016/j.vetmic.2005.12.013
    Silva, L.M.G. ; Baums, C.G. ; Rehm, T. ; Wisselink, H.J. ; Goethe, R. ; Valentin-Weigand, P. / Virulence-associated gene profiling of Streptococcus suis isolates by PCR. In: Veterinary Microbiology. 2006 ; Vol. 115, No. 1-3. pp. 117-127.
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    Silva, LMG, Baums, CG, Rehm, T, Wisselink, HJ, Goethe, R & Valentin-Weigand, P 2006, 'Virulence-associated gene profiling of Streptococcus suis isolates by PCR', Veterinary Microbiology, vol. 115, no. 1-3, pp. 117-127. https://doi.org/10.1016/j.vetmic.2005.12.013

    Virulence-associated gene profiling of Streptococcus suis isolates by PCR. / Silva, L.M.G.; Baums, C.G.; Rehm, T.; Wisselink, H.J.; Goethe, R.; Valentin-Weigand, P.

    In: Veterinary Microbiology, Vol. 115, No. 1-3, 2006, p. 117-127.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Virulence-associated gene profiling of Streptococcus suis isolates by PCR

    AU - Silva, L.M.G.

    AU - Baums, C.G.

    AU - Rehm, T.

    AU - Wisselink, H.J.

    AU - Goethe, R.

    AU - Valentin-Weigand, P.

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    AB - Definition of virulent Streptococcus suis strains is controversial. One successful approach for identification of virulent European strains is differentiation of capsular serotypes (or the corresponding cps types) and subsequent detection of virulence-associated factors, namely the extracellular factor (EF, epf), the muramidase-released protein (MRP, mrp) and the hemolysin suilysin (SLY, sly). In this work we present a novel multiplex PCR (MP-PCR) and an mrp variant PCR for identification and characterization of virulent S. suis strains. These new methods were used to identify association of disease with particular profiles of virulence-associated genes. The MP-PCR allowed identification of S. suis through detection of the housekeeping gene gdh, differentiation of four cps types (1, 2, 7 and 9), and detection of epf, mrp, sly and arcA (arginine deiminase from S. suis). Furthermore, this study describes the first PCR assay for differentiation of at least six mrp variants. Expression of the corresponding size variants of MRP was shown for four of the six mrp variants, but was undetectable for the two larger mrp variants in the particular strains investigated. The results of this study suggest that cps7 strains are associated with pneumonia and that variation of mrp is very pronounced among these strains. Gene profiles of invasive, pneumonia and carrier S. suis isolates by combination of PCR assays allowed differentiation of 24 different genotypes among cps1, 2, 7 and 9 strains. Forty-five percent of the invasive S. suis diseases investigated in this study were caused by only two of these genotypes, namely cps2/mrp+/epf+/sly+ and cps9/mrp*/epf¿/sly+. Thus, this study demonstrates for the first time a uniform profile of the particular virulence-associated genes for the vast majority of the investigated invasive cps9 strains.

    KW - muramidase-released protein

    KW - germ-free pigs

    KW - arginine deiminase system

    KW - tonsillar specimens

    KW - type-2 strains

    KW - diseased pigs

    KW - serotype 7

    KW - identification

    KW - expression

    KW - suilysin

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    M3 - Article

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    EP - 127

    JO - Veterinary Microbiology

    JF - Veterinary Microbiology

    SN - 0378-1135

    IS - 1-3

    ER -