Veterinary Molecular Diagnostics

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

In veterinary molecular diagnostics, samples originating from animals are tested. Developments in the farm animals sector and in our societal attitude towards pet animals have resulted in an increased demand for fast and reliable diagnostic techniques. Molecular diagnostics perfectly matches this increased demand. Veterinary molecular diagnostics primarily focuses on the detection, identification, and genotyping of pathogens. Techniques are comparable to those used in the human molecular diagnostic field. In veterinary diagnosis, these techniques are applied to either the diagnosis of diseases in individual animals and herds or to assess the disease status of a herd. Notable features of veterinary molecular diagnostics are the sampling of a diagnostic unit for herd diagnoses, which can compensate test characteristics and, to a lesser extent, the high RNA/DNA loads in cases of animal disease outbreaks. To further identify bacteria, numerous genotyping techniques are used, including whole genome sequencing (WGS). To characterise viruses, WGS is the method of choice. The applications of molecular techniques in molecular diagnostics and molecular epidemiology are presented in two case studies: Q fever and the highly pathogenic avian influenza
Original languageEnglish
Title of host publicationMolecular Diagnostics Part 2: Clinical, Veterinary, Agrobotanical and Food Safety Applications
EditorsE. van Pelt-Verkuil, W.B. van Leeuwen, R. te Witt
PublisherSpringer
Pages219-234
ISBN (Print)9789811045110
DOIs
Publication statusPublished - 2017

Fingerprint

herds
genotyping
Q fever
methodology
molecular epidemiology
genome
animal diseases
avian influenza
disease diagnosis
farmed animal species
diagnostic techniques
pets
animals
RNA
case studies
sampling
viruses
pathogens
bacteria
DNA

Cite this

Roest, H. I. J., Engelsma, M. Y., Weesendorp, E., Bossers, A., & Elbers, A. R. W. (2017). Veterinary Molecular Diagnostics. In E. van Pelt-Verkuil, W. B. van Leeuwen, & R. te Witt (Eds.), Molecular Diagnostics Part 2: Clinical, Veterinary, Agrobotanical and Food Safety Applications (pp. 219-234). Springer. https://doi.org/10.1007/978-981-10-4511-0_11
Roest, H.I.J. ; Engelsma, M.Y. ; Weesendorp, E. ; Bossers, A. ; Elbers, A.R.W. / Veterinary Molecular Diagnostics. Molecular Diagnostics Part 2: Clinical, Veterinary, Agrobotanical and Food Safety Applications. editor / E. van Pelt-Verkuil ; W.B. van Leeuwen ; R. te Witt. Springer, 2017. pp. 219-234
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Roest, HIJ, Engelsma, MY, Weesendorp, E, Bossers, A & Elbers, ARW 2017, Veterinary Molecular Diagnostics. in E van Pelt-Verkuil, WB van Leeuwen & R te Witt (eds), Molecular Diagnostics Part 2: Clinical, Veterinary, Agrobotanical and Food Safety Applications. Springer, pp. 219-234. https://doi.org/10.1007/978-981-10-4511-0_11

Veterinary Molecular Diagnostics. / Roest, H.I.J.; Engelsma, M.Y.; Weesendorp, E.; Bossers, A.; Elbers, A.R.W.

Molecular Diagnostics Part 2: Clinical, Veterinary, Agrobotanical and Food Safety Applications. ed. / E. van Pelt-Verkuil; W.B. van Leeuwen; R. te Witt. Springer, 2017. p. 219-234.

Research output: Chapter in Book/Report/Conference proceedingChapter

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AB - In veterinary molecular diagnostics, samples originating from animals are tested. Developments in the farm animals sector and in our societal attitude towards pet animals have resulted in an increased demand for fast and reliable diagnostic techniques. Molecular diagnostics perfectly matches this increased demand. Veterinary molecular diagnostics primarily focuses on the detection, identification, and genotyping of pathogens. Techniques are comparable to those used in the human molecular diagnostic field. In veterinary diagnosis, these techniques are applied to either the diagnosis of diseases in individual animals and herds or to assess the disease status of a herd. Notable features of veterinary molecular diagnostics are the sampling of a diagnostic unit for herd diagnoses, which can compensate test characteristics and, to a lesser extent, the high RNA/DNA loads in cases of animal disease outbreaks. To further identify bacteria, numerous genotyping techniques are used, including whole genome sequencing (WGS). To characterise viruses, WGS is the method of choice. The applications of molecular techniques in molecular diagnostics and molecular epidemiology are presented in two case studies: Q fever and the highly pathogenic avian influenza

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Roest HIJ, Engelsma MY, Weesendorp E, Bossers A, Elbers ARW. Veterinary Molecular Diagnostics. In van Pelt-Verkuil E, van Leeuwen WB, te Witt R, editors, Molecular Diagnostics Part 2: Clinical, Veterinary, Agrobotanical and Food Safety Applications. Springer. 2017. p. 219-234 https://doi.org/10.1007/978-981-10-4511-0_11