Venom allergen-like proteins secreted by plant-parasitic nematodes modulate defense responses mediated by extracellular innate immune receptors

J.L. Lozano Torres, R.H.P. Wilbers, S. Warmerdam, A.M. Finkers-Tomczak, C.C. van Schaik, J. Bakker, A. Goverse, A. Schots, G. Smant

Research output: Chapter in Book/Report/Conference proceedingAbstractAcademic

Abstract

Plants and animals utilize cell-surface receptors to survey their direct environment for molecular patterns uniquely associated with infections by microbial invaders. Recently, we showed that a venom allergen-like protein Gr-VAP1 from the plant-parasitic nematode Globodera rostochiensis targets the papain-like cysteine protease Rcr3pim that functions as a cofactor of the extracellular plant immune receptor Cf-2 in tomato. However, the presence of Rcr3pim alone almost doubles the susceptibility of tomato plants to G. rostochiensis, suggesting that Gr-VAP1 modulates the activity of Rcr3pim to enhance the virulence of the nematodes. Although secreted venom allergen-like proteins are highly conserved among plant-parasitic nematodes, their function in parasitism of host plants is largely unknown. Here we demonstrate that venom allergen-like proteins from plant-parasitic nematodes specifically alter defense responses mediated by extracellular innate immune receptors. Knocking-down the expression of venom allergen-like proteins in G. rostochiensis strongly reduced the virulence of the nematodes. Ectopic expression of different venom allergen-like proteins from plant-parasitic nematodes in transgenic Arabidopsis plants significantly reduced their basal defense responses to nematodes, multiple other plant pathogens, and the immunogenic peptide flg22 from bacterial flagella. Furthermore, venom allergen-like proteins altered the defense-related programmed cell death activated by multiple extracellular plant immune receptors in leaves of Nicotiana benthamiana. We therefore propose that venom allergen-like proteins modulate the activation and/or signaling by extracellular immune receptors.
Original languageEnglish
Title of host publicationProceedings of 1st Annual Meeting, COST FA 1208, 09-11 October 2013, Birnam, Scotland
Pages46-46
Publication statusPublished - 2013
EventCOST FA 1208, Pathogen-informed strategies for sustainable broad-spectrum crop resistancem Birnam, Scotland -
Duration: 9 Oct 201311 Oct 2013

Conference

ConferenceCOST FA 1208, Pathogen-informed strategies for sustainable broad-spectrum crop resistancem Birnam, Scotland
Period9/10/1311/10/13

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    Lozano Torres, J. L., Wilbers, R. H. P., Warmerdam, S., Finkers-Tomczak, A. M., van Schaik, C. C., Bakker, J., Goverse, A., Schots, A., & Smant, G. (2013). Venom allergen-like proteins secreted by plant-parasitic nematodes modulate defense responses mediated by extracellular innate immune receptors. In Proceedings of 1st Annual Meeting, COST FA 1208, 09-11 October 2013, Birnam, Scotland (pp. 46-46)