Variation in microbial responses and Rhizoctonia solani AG2-2IIIB growth in soil under different organic amendment regimes

L.S. van Overbeek, I. Senechkin, A.H.C. van Bruggen

    Research output: Contribution to journalArticleAcademicpeer-review

    9 Citations (Scopus)

    Abstract

    Organic amendments influence chemical and microbial compositions in soils and also susceptibility to plant diseases. The purpose of this study was to establish different parameters that interfere with pathogen growth in soil. Four different organic-amendment regimes, i.e. slurry, compost, slurry-dung and compost-slurry-dung, were applied to two fields proximate to each other on the same farm and covered with the same grass-clover ley in the last two years preceding sampling. Before that period, there were differences in cropping and management practices between both fields. Chemical analyses of the soils revealed no differences between organic amendments, whereas significant differences were present between fields in all C, N and pH values. Growth of R. solani AG2-2IIIB in soil, measured by damping-off in sugar beet plants, was influenced by the interaction of organic amendment with field type. Diversity and evenness values of the microbial communities, studied by PCR-DGGEs specific for bacteria, fungi, Pseudomonas and ammonia-oxidizing beta-proteobacteria, revealed different patterns, i.e. no differences between organic amendments, but clear differences between fields. Multivariate analyses done on individual species of the four groups, as represented by band location and size in PCR-DGGE fingerprints, and by inclusion of chemical and R. solani AG2-2IIIB growth parameters as `environmental' variables, revealed strong and occasionally significant effects of organic matter content, water-dissolvable organic carbon and pH on microbial communities. It was therefore concluded that different organic amendments had different effects on pathogen growth in soils of both fields and that organic matter content and pH influenced soil microbial compositions most.
    Original languageEnglish
    Pages (from-to)268-276
    JournalCanadian Journal of Plant Pathology = Revue Canadienne de Phytopathologie
    Volume34
    Issue number2
    DOIs
    Publication statusPublished - 2012

    Keywords

    • gradient gel-electrophoresis
    • ammonia-oxidizing bacteria
    • 16s ribosomal-rna
    • ralstonia-solanacearum biovar-2
    • fluorescent pseudomonads
    • nitrogen fertilizers
    • disease suppression
    • soilborne diseases
    • take-all
    • communities

    Fingerprint Dive into the research topics of 'Variation in microbial responses and Rhizoctonia solani AG2-2IIIB growth in soil under different organic amendment regimes'. Together they form a unique fingerprint.

  • Projects

    Nematocure (KB-12-007.03-001)

    van Overbeek, L.

    5/12/1231/12/15

    Project: EZproject

    Cite this