Variation in accumulation of isoflavonoids in Phaseoleae seedlings elicited by Rhizopus

Siti Aisyah, Harry Gruppen, Silvia Andini, Monique Bettonvil, Eduard Severing, Jean Paul Vincken*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

Seeds from seven species of tribe Phaseoleae, i.e. Phaseolus, Vigna, Lablab and Psophocarpus, were investigated for inducibility of isoflavonoids by germination with or without subsequent elicitation with Rhizopus oryzae. Germination alone poorly induced isoflavonoid production (in the range of 0.2-0.7 mg representative compound equivalents (RCE)/g DW), whereas application of Rhizopus onto the seedlings increased the isoflavonoid content considerably (in the range of 0.5-3.3 mg RCE/g DW). The inducibility of different isoflavonoid subclasses in seedlings with Rhizopus varied per species. Isoflavones and isoflavanones were mainly found in elicited seedlings of Phaseolus, Vigna and Lablab, whereas pterocarpans were mainly observed in those of Psophocarpus. Despite their phylogenetic relatedness, the seeds of various species within Phaseoleae appeared to respond differently towards elicitation by Rhizopus during germination. The kind of molecules induced followed the phylogenetic relationship of the various species, but their amounts induced during germination, alone or combined with elicitation, did not.

Original languageEnglish
Pages (from-to)694-701
JournalFood Chemistry
Volume196
DOIs
Publication statusPublished - 1 Apr 2016

Keywords

  • Flavonoid
  • Germination
  • Mass spectrometry
  • Phylogenetic relationship
  • Phytoalexin
  • Prenylation

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