Valorization of jatropha fruit biomass for energy applications

A. Marasabessy

Research output: Thesisinternal PhD, WUAcademic

Abstract

Valorization of Jatropha fruit biomass for

energy applications

Ahmad Marasabessy

Thesis Abstract

Our research objectives were to develop sustainable technologies of Jatropha oil extraction and Jatropha biomass fractionation within a framework of bioconversions (enzymatic and microbial processings).  Microbial extraction of oil from Jatropha kernels using whole cells of Bacillus pumilus yields 73% oil, and this is comparable to the known processes such as by using expeller or by enzymatic extraction. The bacterium facilitates oil liberation via degradation of hemicelluloses therefore the majority of Jatropha proteins were preserved in the solid phase of the extraction residues. In investigating the effect of dilute sulfuric acid pretreatment on the enzymatic digestibility of the lignocellulosic components of Jatropha fruit biomass, we found that the seed shell and the seed cake were more recalcitrant to dilute sulfuric acid pretreatments than the fruit hull. A pretreatment of the fruit hull at optimum conditions (10% solid loading, 0.9% sulfuric acid, 30 min, 178 oC) followed by neutralization and a 24-h enzymatic hydrolysis with cellulases (GC220) liberated 100% pentoses (71% yield and 29% degradation to furfural) and 83% hexoses (78% yield and 5% degradation to 5-hydroxymethylfurfural). The fruit hull hydrolyzate can be used as a substrate for Saccharomyces cerevisiae to produce ethanol in SSF process. Our economic analysis in the retrospectives showed that valorization of the fruit biomass into variuos products (oil, protein isolate, lignin, biogas, bio-oil, etc.) using the most known techniques (pretreatment, hydrolysis, fermentation, extraction, separation, anaerobic digestion, pyrolysis) could improve the economy value of this biofuel crop significantly.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Sanders, Johan, Promotor
  • Weusthuis, Ruud, Co-promotor
  • Moeis, M., Co-promotor, External person
Award date2 Apr 2015
Place of PublicationWageningen
Publisher
Print ISBNs9789462572614
Publication statusPublished - 2015

Fingerprint

Jatropha
bioenergy
hulls
oils
fruits
sulfuric acid
pretreatment
enzymatic hydrolysis
degradation
biomass
seeds
sustainable technology
Bacillus pumilus
furfural
hydroxymethylfurfural
cellulases
pentoses
anaerobic digestion
protein isolates
energy crops

Keywords

  • agricultural byproducts
  • jatropha curcas
  • bioenergy
  • biorefinery
  • biofuels
  • economic aspects
  • extraction
  • fractionation
  • hydrolysis
  • indonesia

Cite this

Marasabessy, A. (2015). Valorization of jatropha fruit biomass for energy applications. Wageningen: Wageningen University.
Marasabessy, A.. / Valorization of jatropha fruit biomass for energy applications. Wageningen : Wageningen University, 2015. 147 p.
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title = "Valorization of jatropha fruit biomass for energy applications",
abstract = "Valorization of Jatropha fruit biomass for energy applications Ahmad Marasabessy Thesis Abstract Our research objectives were to develop sustainable technologies of Jatropha oil extraction and Jatropha biomass fractionation within a framework of bioconversions (enzymatic and microbial processings).  Microbial extraction of oil from Jatropha kernels using whole cells of Bacillus pumilus yields 73{\%} oil, and this is comparable to the known processes such as by using expeller or by enzymatic extraction. The bacterium facilitates oil liberation via degradation of hemicelluloses therefore the majority of Jatropha proteins were preserved in the solid phase of the extraction residues. In investigating the effect of dilute sulfuric acid pretreatment on the enzymatic digestibility of the lignocellulosic components of Jatropha fruit biomass, we found that the seed shell and the seed cake were more recalcitrant to dilute sulfuric acid pretreatments than the fruit hull. A pretreatment of the fruit hull at optimum conditions (10{\%} solid loading, 0.9{\%} sulfuric acid, 30 min, 178 oC) followed by neutralization and a 24-h enzymatic hydrolysis with cellulases (GC220) liberated 100{\%} pentoses (71{\%} yield and 29{\%} degradation to furfural) and 83{\%} hexoses (78{\%} yield and 5{\%} degradation to 5-hydroxymethylfurfural). The fruit hull hydrolyzate can be used as a substrate for Saccharomyces cerevisiae to produce ethanol in SSF process. Our economic analysis in the retrospectives showed that valorization of the fruit biomass into variuos products (oil, protein isolate, lignin, biogas, bio-oil, etc.) using the most known techniques (pretreatment, hydrolysis, fermentation, extraction, separation, anaerobic digestion, pyrolysis) could improve the economy value of this biofuel crop significantly.",
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author = "A. Marasabessy",
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Marasabessy, A 2015, 'Valorization of jatropha fruit biomass for energy applications', Doctor of Philosophy, Wageningen University, Wageningen.

Valorization of jatropha fruit biomass for energy applications. / Marasabessy, A.

Wageningen : Wageningen University, 2015. 147 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Valorization of jatropha fruit biomass for energy applications

AU - Marasabessy, A.

N1 - WU thesis 6004

PY - 2015

Y1 - 2015

N2 - Valorization of Jatropha fruit biomass for energy applications Ahmad Marasabessy Thesis Abstract Our research objectives were to develop sustainable technologies of Jatropha oil extraction and Jatropha biomass fractionation within a framework of bioconversions (enzymatic and microbial processings).  Microbial extraction of oil from Jatropha kernels using whole cells of Bacillus pumilus yields 73% oil, and this is comparable to the known processes such as by using expeller or by enzymatic extraction. The bacterium facilitates oil liberation via degradation of hemicelluloses therefore the majority of Jatropha proteins were preserved in the solid phase of the extraction residues. In investigating the effect of dilute sulfuric acid pretreatment on the enzymatic digestibility of the lignocellulosic components of Jatropha fruit biomass, we found that the seed shell and the seed cake were more recalcitrant to dilute sulfuric acid pretreatments than the fruit hull. A pretreatment of the fruit hull at optimum conditions (10% solid loading, 0.9% sulfuric acid, 30 min, 178 oC) followed by neutralization and a 24-h enzymatic hydrolysis with cellulases (GC220) liberated 100% pentoses (71% yield and 29% degradation to furfural) and 83% hexoses (78% yield and 5% degradation to 5-hydroxymethylfurfural). The fruit hull hydrolyzate can be used as a substrate for Saccharomyces cerevisiae to produce ethanol in SSF process. Our economic analysis in the retrospectives showed that valorization of the fruit biomass into variuos products (oil, protein isolate, lignin, biogas, bio-oil, etc.) using the most known techniques (pretreatment, hydrolysis, fermentation, extraction, separation, anaerobic digestion, pyrolysis) could improve the economy value of this biofuel crop significantly.

AB - Valorization of Jatropha fruit biomass for energy applications Ahmad Marasabessy Thesis Abstract Our research objectives were to develop sustainable technologies of Jatropha oil extraction and Jatropha biomass fractionation within a framework of bioconversions (enzymatic and microbial processings).  Microbial extraction of oil from Jatropha kernels using whole cells of Bacillus pumilus yields 73% oil, and this is comparable to the known processes such as by using expeller or by enzymatic extraction. The bacterium facilitates oil liberation via degradation of hemicelluloses therefore the majority of Jatropha proteins were preserved in the solid phase of the extraction residues. In investigating the effect of dilute sulfuric acid pretreatment on the enzymatic digestibility of the lignocellulosic components of Jatropha fruit biomass, we found that the seed shell and the seed cake were more recalcitrant to dilute sulfuric acid pretreatments than the fruit hull. A pretreatment of the fruit hull at optimum conditions (10% solid loading, 0.9% sulfuric acid, 30 min, 178 oC) followed by neutralization and a 24-h enzymatic hydrolysis with cellulases (GC220) liberated 100% pentoses (71% yield and 29% degradation to furfural) and 83% hexoses (78% yield and 5% degradation to 5-hydroxymethylfurfural). The fruit hull hydrolyzate can be used as a substrate for Saccharomyces cerevisiae to produce ethanol in SSF process. Our economic analysis in the retrospectives showed that valorization of the fruit biomass into variuos products (oil, protein isolate, lignin, biogas, bio-oil, etc.) using the most known techniques (pretreatment, hydrolysis, fermentation, extraction, separation, anaerobic digestion, pyrolysis) could improve the economy value of this biofuel crop significantly.

KW - landbouwbijproducten

KW - jatropha curcas

KW - bio-energie

KW - bioraffinage

KW - biobrandstoffen

KW - economische aspecten

KW - extractie

KW - fractionering

KW - hydrolyse

KW - indonesië

KW - agricultural byproducts

KW - jatropha curcas

KW - bioenergy

KW - biorefinery

KW - biofuels

KW - economic aspects

KW - extraction

KW - fractionation

KW - hydrolysis

KW - indonesia

M3 - internal PhD, WU

SN - 9789462572614

PB - Wageningen University

CY - Wageningen

ER -

Marasabessy A. Valorization of jatropha fruit biomass for energy applications. Wageningen: Wageningen University, 2015. 147 p.