Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study

K.L. Capps, E.M. McLaughlin, A.W.A. Murray, C.F. Aldus, G.M. Wyatt, M.W. Peck, A. van Amerongen, R.M.C. Ariens, J.H. Wichers, C.L. Bayliss, D.R.A. Wareing, F.J. Bolton

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    Abstract

    An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (
    Original languageEnglish
    Pages (from-to)68-77
    JournalJournal of AOAC International
    Volume87
    Issue number1.
    Publication statusPublished - 2004

    Keywords

    • england
    • wales
    • o157
    • vtec

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  • Cite this

    Capps, K. L., McLaughlin, E. M., Murray, A. W. A., Aldus, C. F., Wyatt, G. M., Peck, M. W., van Amerongen, A., Ariens, R. M. C., Wichers, J. H., Bayliss, C. L., Wareing, D. R. A., & Bolton, F. J. (2004). Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study. Journal of AOAC International, 87(1.), 68-77.