An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (
|Journal||Journal of AOAC International|
|Publication status||Published - 2004|
Capps, K. L., McLaughlin, E. M., Murray, A. W. A., Aldus, C. F., Wyatt, G. M., Peck, M. W., van Amerongen, A., Ariens, R. M. C., Wichers, J. H., Bayliss, C. L., Wareing, D. R. A., & Bolton, F. J. (2004). Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study. Journal of AOAC International, 87(1.), 68-77.