Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study

K.L. Capps; E.M. McLaughlin; A.W.A. Murray; C.F. Aldus; G.M. Wyatt; M.W. Peck; A. van Amerongen; R.M.C. Ariens; J.H. Wichers; C.L. Bayliss; D.R.A. Wareing; F.J. Bolton

Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study

K.L. Capps, E.M. McLaughlin, A.W.A. Murray, C.F. Aldus, G.M. Wyatt, M.W. Peck, A. van Amerongen, R.M.C. Ariens, J.H. Wichers, C.L. Bayliss, D.R.A. Wareing, F.J. Bolton

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Abstract

An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (

Original language	English
Pages (from-to)	68-77
Journal	Journal of AOAC International
Volume	87
Issue number	1.
Publication status	Published - 2004

Keywords

england
wales
o157
vtec

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Capps, K. L., McLaughlin, E. M., Murray, A. W. A., Aldus, C. F., Wyatt, G. M., Peck, M. W., van Amerongen, A., Ariens, R. M. C., Wichers, J. H., Bayliss, C. L., Wareing, D. R. A., & Bolton, F. J. (2004). Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study. Journal of AOAC International, 87(1.), 68-77.

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abstract = "An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (",

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AU - Capps, K.L.

AU - McLaughlin, E.M.

AU - Murray, A.W.A.

AU - Aldus, C.F.

AU - Wyatt, G.M.

AU - Peck, M.W.

AU - van Amerongen, A.

AU - Ariens, R.M.C.

AU - Wichers, J.H.

AU - Bayliss, C.L.

AU - Wareing, D.R.A.

AU - Bolton, F.J.

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AB - An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (

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KW - wales

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Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study

Abstract

Keywords

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