Validation of an analytical method for the determination of spiramycin, virginiamycin and tylosin in feeding-stuffs bij thin-layer chromatography and bio-autography

U. Vincent, G. Gizzi, C. von Holst, J. de Jong, J. Michard

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

An inter-laboratory validation was carried out to determine the performance characteristics of an analytical method based on thin-layer chromatography (TLC) coupled to microbiological detection (bio-autography) for screening feed samples for the presence of spiramycin, tylosin and virginiamycin. Twenty-four samples including blank samples and samples with concentrations of the target analytes ranging between 1 and 5 mg kg(-1) (expressed in microbiological activity) were analysed by seven laboratories participating in the study. The required detection limit was 1 mg kg(-1) (expressed in microbiological activity). For spiramycin, acceptable values for the sensitivity (at least 95%) indicating the rate of correct positive results were obtained for samples containing this substance at or above 2 mg kg(-1), whereas at 1 mg kg(-1), the sensitivity rate dropped to about 70%. Therefore, it was concluded that the detection limit was 2 mg kg(-1). For tylosin and virginiamycin, acceptable values of the sensitivity were obtained for all concentrations including 1 mg kg(-1). Therefore, the method fulfils the criterion regarding the required sensitivity at the target detection limit for tylosin and virginiamycin.
Original languageEnglish
Pages (from-to)351-359
Number of pages9
JournalFood Additives and Contaminants
Volume24
Issue number4
DOIs
Publication statusPublished - 2007

Keywords

  • animal feeds

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