Validation of a recombinant cell bioassay for the detection of (gluco)corticosteroids in feed

T.F.H. Bovee, H.H. Heskamp, J.R. Helsdingen, A.R.M. Hamers, B.A. Brouwer, M.W.F. Nielen

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Abstract

Use of hormones for fattening purposes is forbidden in the animal production in Europe (European Commission. 1996. Council Directive EC/96/22 (replacement of 88/146/EC). Off J Eur Commun. L125:3–9; European Commission. 1996. Council Directive EC/96/23. Off J Eur Commun. L125:10–32). Moreover, Regulation (EC) 178/2002 (European Commission. 2002. Regulation EC No 178/2002. Off J Eur Commun. L31:1–24) and Regulation (EC) 882/2004 (European Commission. 2004. Regulation EC No 882/2004. Off J Eur Commun. L165:1–135) oblige the member states to identify emerging risks and use validated and accredited methods for control analysis. Only combinations of bioassay activity screening with chemical identification are suited to uphold all laws. No such combination is described for the detection of (gluco)corticoids. In the present study, the GR-CALUX bioassay was validated as a qualitative screening method for the determination of glucocorticoid activity in feed. This validation was performed according to EC Decision 2002/657/EC (European Commission. 2002. Commission Decision 2002/657/EC from Directive 96/23. Off J Eur Commun. L221:8–36). Twenty-two representative blank feed samples were selected and spiked with 50¿ng¿g-1 of dexamethasone, 100¿ng¿g-1 of betamethasone or 500¿ng¿g-1 of triamcinolone. All blank and spiked feed samples fulfilled the CCa and CCß criteria; the method was specific and robust and glucocorticoids in feed were stable for at least 88 days.
Original languageEnglish
Pages (from-to)264-271
JournalFood Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment
Volume30
Issue number2
DOIs
Publication statusPublished - 2013

Fingerprint

Bioassay
adrenal cortex hormones
Biological Assay
Glucocorticoids
Adrenal Cortex Hormones
Screening
bioassays
Triamcinolone
Betamethasone
glucocorticoids
Dexamethasone
Galectin 3
Animals
betamethasone
triamcinolone
Hormones
cells
screening
dexamethasone
animal production

Keywords

  • glucocorticoid receptor
  • liquid-chromatography
  • mass-spectrometry
  • growth promoters
  • calf urine
  • performance
  • induction
  • steroids

Cite this

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title = "Validation of a recombinant cell bioassay for the detection of (gluco)corticosteroids in feed",
abstract = "Use of hormones for fattening purposes is forbidden in the animal production in Europe (European Commission. 1996. Council Directive EC/96/22 (replacement of 88/146/EC). Off J Eur Commun. L125:3–9; European Commission. 1996. Council Directive EC/96/23. Off J Eur Commun. L125:10–32). Moreover, Regulation (EC) 178/2002 (European Commission. 2002. Regulation EC No 178/2002. Off J Eur Commun. L31:1–24) and Regulation (EC) 882/2004 (European Commission. 2004. Regulation EC No 882/2004. Off J Eur Commun. L165:1–135) oblige the member states to identify emerging risks and use validated and accredited methods for control analysis. Only combinations of bioassay activity screening with chemical identification are suited to uphold all laws. No such combination is described for the detection of (gluco)corticoids. In the present study, the GR-CALUX bioassay was validated as a qualitative screening method for the determination of glucocorticoid activity in feed. This validation was performed according to EC Decision 2002/657/EC (European Commission. 2002. Commission Decision 2002/657/EC from Directive 96/23. Off J Eur Commun. L221:8–36). Twenty-two representative blank feed samples were selected and spiked with 50¿ng¿g-1 of dexamethasone, 100¿ng¿g-1 of betamethasone or 500¿ng¿g-1 of triamcinolone. All blank and spiked feed samples fulfilled the CCa and CC{\ss} criteria; the method was specific and robust and glucocorticoids in feed were stable for at least 88 days.",
keywords = "glucocorticoid receptor, liquid-chromatography, mass-spectrometry, growth promoters, calf urine, performance, induction, steroids",
author = "T.F.H. Bovee and H.H. Heskamp and J.R. Helsdingen and A.R.M. Hamers and B.A. Brouwer and M.W.F. Nielen",
year = "2013",
doi = "10.1080/19440049.2012.745098",
language = "English",
volume = "30",
pages = "264--271",
journal = "Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment",
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TY - JOUR

T1 - Validation of a recombinant cell bioassay for the detection of (gluco)corticosteroids in feed

AU - Bovee, T.F.H.

AU - Heskamp, H.H.

AU - Helsdingen, J.R.

AU - Hamers, A.R.M.

AU - Brouwer, B.A.

AU - Nielen, M.W.F.

PY - 2013

Y1 - 2013

N2 - Use of hormones for fattening purposes is forbidden in the animal production in Europe (European Commission. 1996. Council Directive EC/96/22 (replacement of 88/146/EC). Off J Eur Commun. L125:3–9; European Commission. 1996. Council Directive EC/96/23. Off J Eur Commun. L125:10–32). Moreover, Regulation (EC) 178/2002 (European Commission. 2002. Regulation EC No 178/2002. Off J Eur Commun. L31:1–24) and Regulation (EC) 882/2004 (European Commission. 2004. Regulation EC No 882/2004. Off J Eur Commun. L165:1–135) oblige the member states to identify emerging risks and use validated and accredited methods for control analysis. Only combinations of bioassay activity screening with chemical identification are suited to uphold all laws. No such combination is described for the detection of (gluco)corticoids. In the present study, the GR-CALUX bioassay was validated as a qualitative screening method for the determination of glucocorticoid activity in feed. This validation was performed according to EC Decision 2002/657/EC (European Commission. 2002. Commission Decision 2002/657/EC from Directive 96/23. Off J Eur Commun. L221:8–36). Twenty-two representative blank feed samples were selected and spiked with 50¿ng¿g-1 of dexamethasone, 100¿ng¿g-1 of betamethasone or 500¿ng¿g-1 of triamcinolone. All blank and spiked feed samples fulfilled the CCa and CCß criteria; the method was specific and robust and glucocorticoids in feed were stable for at least 88 days.

AB - Use of hormones for fattening purposes is forbidden in the animal production in Europe (European Commission. 1996. Council Directive EC/96/22 (replacement of 88/146/EC). Off J Eur Commun. L125:3–9; European Commission. 1996. Council Directive EC/96/23. Off J Eur Commun. L125:10–32). Moreover, Regulation (EC) 178/2002 (European Commission. 2002. Regulation EC No 178/2002. Off J Eur Commun. L31:1–24) and Regulation (EC) 882/2004 (European Commission. 2004. Regulation EC No 882/2004. Off J Eur Commun. L165:1–135) oblige the member states to identify emerging risks and use validated and accredited methods for control analysis. Only combinations of bioassay activity screening with chemical identification are suited to uphold all laws. No such combination is described for the detection of (gluco)corticoids. In the present study, the GR-CALUX bioassay was validated as a qualitative screening method for the determination of glucocorticoid activity in feed. This validation was performed according to EC Decision 2002/657/EC (European Commission. 2002. Commission Decision 2002/657/EC from Directive 96/23. Off J Eur Commun. L221:8–36). Twenty-two representative blank feed samples were selected and spiked with 50¿ng¿g-1 of dexamethasone, 100¿ng¿g-1 of betamethasone or 500¿ng¿g-1 of triamcinolone. All blank and spiked feed samples fulfilled the CCa and CCß criteria; the method was specific and robust and glucocorticoids in feed were stable for at least 88 days.

KW - glucocorticoid receptor

KW - liquid-chromatography

KW - mass-spectrometry

KW - growth promoters

KW - calf urine

KW - performance

KW - induction

KW - steroids

U2 - 10.1080/19440049.2012.745098

DO - 10.1080/19440049.2012.745098

M3 - Article

VL - 30

SP - 264

EP - 271

JO - Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment

JF - Food Additives & Contaminants. Pt. A, Chemistry, Analysis, Control, Exposure & Risk Assessment

SN - 1944-0049

IS - 2

ER -