Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis)

K. Cankar, A.M.M.L. van Houwelingen, M.A. Goedbloed, R. Renirie, R.M. de Jong, H.J. Bouwmeester, H.J. Bosch, Th. Sonke, M.J. Beekwilder

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30 Citations (Scopus)


(+)-Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar (Callitropsis nootkatensis) heartwood essential oil. Upon co-expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans-nootkatol and (+)-nootkatone. Formation of (+)-nootkatone was detected at 144 ± 10 lg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases.
Original languageEnglish
Pages (from-to)1001-1007
JournalFEBS Letters
Issue number6
Publication statusPublished - 2014


  • essential oil
  • functional-characterization
  • ixodes-scapularis
  • cytochrome-p450 monooxygenase
  • amblyomma-americanum
  • yeast expression
  • acari ixodidae
  • yellow-cedar
  • mint mentha
  • nootkatone

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