Abstract
(+)-Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar (Callitropsis nootkatensis) heartwood essential oil. Upon co-expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was
identified to produce trans-nootkatol and (+)-nootkatone. Formation of (+)-nootkatone was detected at 144 ± 10 lg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases.
Original language | English |
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Pages (from-to) | 1001-1007 |
Journal | FEBS Letters |
Volume | 588 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- essential oil
- functional-characterization
- ixodes-scapularis
- cytochrome-p450 monooxygenase
- amblyomma-americanum
- yeast expression
- acari ixodidae
- yellow-cedar
- mint mentha
- nootkatone