Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis)

K. Cankar, A.M.M.L. van Houwelingen, M.A. Goedbloed, R. Renirie, R.M. de Jong, H.J. Bouwmeester, H.J. Bosch, Th. Sonke, M.J. Beekwilder

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

(+)-Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar (Callitropsis nootkatensis) heartwood essential oil. Upon co-expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans-nootkatol and (+)-nootkatone. Formation of (+)-nootkatone was detected at 144 ± 10 lg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases.
LanguageEnglish
Pages1001-1007
JournalFEBS Letters
Volume588
Issue number6
DOIs
Publication statusPublished - 2014

Fingerprint

Cytochrome P-450 Enzyme System
Citrus paradisi
Oxidoreductases
Yeast
Yeasts
Sesquiterpenes
Citrus
Flavors
Electron Transport Complex IV
Volatile Oils
Ketones
Chemical analysis
nootkatone
valencene
Nootkatol

Keywords

  • essential oil
  • functional-characterization
  • ixodes-scapularis
  • cytochrome-p450 monooxygenase
  • amblyomma-americanum
  • yeast expression
  • acari ixodidae
  • yellow-cedar
  • mint mentha
  • nootkatone

Cite this

Cankar, K. ; van Houwelingen, A.M.M.L. ; Goedbloed, M.A. ; Renirie, R. ; de Jong, R.M. ; Bouwmeester, H.J. ; Bosch, H.J. ; Sonke, Th. ; Beekwilder, M.J. / Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis). In: FEBS Letters. 2014 ; Vol. 588, No. 6. pp. 1001-1007.
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abstract = "(+)-Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar (Callitropsis nootkatensis) heartwood essential oil. Upon co-expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans-nootkatol and (+)-nootkatone. Formation of (+)-nootkatone was detected at 144 ± 10 lg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases.",
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Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis). / Cankar, K.; van Houwelingen, A.M.M.L.; Goedbloed, M.A.; Renirie, R.; de Jong, R.M.; Bouwmeester, H.J.; Bosch, H.J.; Sonke, Th.; Beekwilder, M.J.

In: FEBS Letters, Vol. 588, No. 6, 2014, p. 1001-1007.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Valencene oxidase CYP706M1 from Alaska cedar (Callitropsis nootkatensis)

AU - Cankar, K.

AU - van Houwelingen, A.M.M.L.

AU - Goedbloed, M.A.

AU - Renirie, R.

AU - de Jong, R.M.

AU - Bouwmeester, H.J.

AU - Bosch, H.J.

AU - Sonke, Th.

AU - Beekwilder, M.J.

PY - 2014

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AB - (+)-Nootkatone is a natural sesquiterpene ketone used in grapefruit and citrus flavour compositions. It occurs in small amounts in grapefruit and is a major component of Alaska cedar (Callitropsis nootkatensis) heartwood essential oil. Upon co-expression of candidate cytochrome P450 enzymes from Alaska cedar in yeast with a valencene synthase, a C. nootkatensis valencene oxidase (CnVO) was identified to produce trans-nootkatol and (+)-nootkatone. Formation of (+)-nootkatone was detected at 144 ± 10 lg/L yeast culture. CnVO belongs to a new subfamily of the CYP706 family of cytochrome P450 oxidases.

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KW - functional-characterization

KW - ixodes-scapularis

KW - cytochrome-p450 monooxygenase

KW - amblyomma-americanum

KW - yeast expression

KW - acari ixodidae

KW - yellow-cedar

KW - mint mentha

KW - nootkatone

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