Utilization of aminoaromatic acids by a methanogenic enrichment culture and by a novel Citrobacter freundii strain

O. Savelieva, I. Kotova, W. Roelofsen, A.J.M. Stams, A. Netrusov

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Abstract

Following incubation of mesophilic methanogenic floccular sludge from a lab-scale upflow anaerobic sludge bed reactor used to treat cattle manure wastewater, a stable 5-aminosalicylate-degrading enrichment culture was obtained. Subsequently, a Citrobacter freundii strain, WA1, was isolated from the 5-aminosalicylate-degrading methanogenic consortium. The methanogenic enrichment culture degraded 5-aminosalicylate completely to CH4, CO2 and NH4+, while C. freundii strain WA1 reduced 5-aminosalicylate with simultaneous deamination to 2-hydroxybenzyl alcohol during anaerobic growth with electron donors such as pyruvate, glucose or serine. When grown on pyruvate, C. freundii WA1 converted 3-aminobenzoate to benzyl alcohol and also reduced benzaldehyde to benzyl alcohol. Pyruvate was fermented to acetate, CO2, H-2 and small amounts of lactate, succinate and formate. Less lactate (30%) was produced from pyruvate when C. freundii WA1 grew with 5-aminosalicylate as co-substrate.
Original languageEnglish
Pages (from-to)163-170
JournalArchives of Microbiology
Volume181
Issue number2
DOIs
Publication statusPublished - 2004

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Keywords

  • anaerobic degradation
  • corresponding alcohols
  • reduction
  • biodegradation
  • products
  • bacteria
  • rna

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