Using poly(β-hydroxybutyrate-β-hydroxyvalerate) as carbon source in biofloc-systems: Nitrogen dynamics and shift of Oreochromis niloticus gut microbiota

Gang Liu, Yale Deng, Marc Verdegem, Zhangying Ye, Songming Zhu*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)

Abstract

Inorganic‑nitrogen removal is essential for the sustainable operation of aquaculture industry and also influences the health of aquatic animals, which may be accomplished by utilizing biofloc technology. In this paper, we studied the use of three different carbon sources 1) longan seed powder (LP), 2) Poly(β-hydroxybutyrate-β-hydroxyvalerate) (PHBV) and 3) synthesized PHBV and LP (PHBVL) in biofloc systems for 90 days to investigate the nitrogen dynamics and gut microbiota of Nile tilapia (Oreochromis niloticus). The PHBVL and PHBV groups had higher total inorganic‑nitrogen removal efficiencies (70.99 ± 19.45% and 63.54 ± 19.44%) than the LP group (35.02 ± 11.21%), which had an accumulation of nitrate. Meanwhile, the biofloc in PHBVL and PHBV group generally had a higher amino acid composition, particularly for methionine and lysine, but was not reflected in the tilapia muscle. High-throughput sequencing indicated that the different carbohydrates shaped different bacterial community compositions in the fish gut after exposure in the three environments for 90-day. These differences, which resulted in different gut digestive enzyme activities (amylase, lipase and trypsin), and growth performance, which the food conversion ratio in the PHBVL group was lower than LP and PHBV group, the final body weight in PHBVL group was average 4.33% and 3.65% bigger than in LP and PHBV group. Network analysis revealed that the keystone taxa (90.33%) were Proteobacteria, Chloroflexi, Actinobacteria, Planctomycetes, Verrucomicrobia and Bacteroidetes, which relative abundance varied in the fish gut in the three groups. The experiment verified the feasibility and advantage to use biodegradable polymers (BDPs) as carbohydrates for biofloc systems.

Original languageEnglish
Article number133664
JournalScience of the Total Environment
Volume694
DOIs
Publication statusPublished - 1 Dec 2019

Keywords

  • Biodegradable polymers (BDPs)
  • Biofloc system
  • Carbohydrate
  • Gut microbiota
  • Nitrogen dynamic

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