Using Lactococcus lactis for glutathione overproduction

Y. Li, J. Hugenholtz, W. Sybesma, T. Abee, D. Molenaar

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Scopus)

Abstract

Glutathione and -glutamylcysteine were produced in Lactococcus lactis using a controlled expression system and the genes gshA and gshB from Escherichia coli encoding the enzymes -glutamylcysteine synthetase and glutathione synthetase. High levels of -glutamylcysteine were found in strains growing on chemically defined medium and expressing either gshA alone or both gshA and gshB. As anticipated, glutathione was found in a strain expressing gshA and gshB. The level of glutathione production could be increased by addition of the precursor amino acid cysteine to the medium. The addition of cysteine led to an increased activity of glutathione synthetase, which is remarkable because the amino acid is not a substrate of this enzyme. The final intracellular glutathione concentration attained was 358 nmol mg¿1 protein, which is the highest concentration reported for a bacterium, demonstrating the suitability of engineered L. lactis for fine-chemical production and as a model for studies of the impact of glutathione on flavour formation and other properties of food.
Original languageEnglish
Pages (from-to)83-90
JournalApplied Microbiology and Biotechnology
Volume67
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • escherichia-coli-b
  • gamma-glutamylcysteine synthetase
  • molecular-weight thiols
  • acid bacteria
  • saccharomyces-cerevisiae
  • nucleotide-sequence
  • oxidative stress
  • gene
  • expression
  • metabolism

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