Use of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy

S.B. Orlinkskii; I.V. Borovykh; V. Zielke; H.J. Steinhoff

doi:10.1134/S0021364007140172

Use of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy

S.B. Orlinkskii, I.V. Borovykh, V. Zielke, H.J. Steinhoff

Biophysics

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The applicability of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy is demonstrated. With the use of bacteriorhodopsin embedded in a lipid membrane as an example, the spectra of protons of neighboring amino acids are recorded, electric field gradients at the membrane surface are detected, and the constant of hyperfine interaction with the chlorine nucleus at the site of ion trapping is measured.

Original language	English
Pages (from-to)	149-152
Journal	JETP Letters
Volume	86
Issue number	2
DOIs	https://doi.org/10.1134/S0021364007140172
Publication status	Published - 2007

Keywords

high-field epr
bacteriorhodopsin
proton

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10.1134/S0021364007140172

Cite this

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title = "Use of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy",

abstract = "The applicability of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy is demonstrated. With the use of bacteriorhodopsin embedded in a lipid membrane as an example, the spectra of protons of neighboring amino acids are recorded, electric field gradients at the membrane surface are detected, and the constant of hyperfine interaction with the chlorine nucleus at the site of ion trapping is measured.",

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T1 - Use of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy

AU - Orlinkskii, S.B.

AU - Borovykh, I.V.

AU - Zielke, V.

AU - Steinhoff, H.J.

PY - 2007

Y1 - 2007

N2 - The applicability of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy is demonstrated. With the use of bacteriorhodopsin embedded in a lipid membrane as an example, the spectra of protons of neighboring amino acids are recorded, electric field gradients at the membrane surface are detected, and the constant of hyperfine interaction with the chlorine nucleus at the site of ion trapping is measured.

AB - The applicability of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy is demonstrated. With the use of bacteriorhodopsin embedded in a lipid membrane as an example, the spectra of protons of neighboring amino acids are recorded, electric field gradients at the membrane surface are detected, and the constant of hyperfine interaction with the chlorine nucleus at the site of ion trapping is measured.

KW - high-field epr

KW - bacteriorhodopsin

KW - proton

U2 - 10.1134/S0021364007140172

DO - 10.1134/S0021364007140172

M3 - Article

SN - 0021-3640

VL - 86

SP - 149

EP - 152

JO - JETP Letters

JF - JETP Letters

IS - 2

ER -

Use of spin labels to study membrane proteins by high-frequency electron nuclear double resonance spectroscopy

Abstract

Keywords

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