Up- and downregulation of Fragaria x ananassa O-methyltransferase: Impacts on Furanone and Phenylpropanoid metabolism

S. Lunkenbein, E.M.J. Salentijn, H. Coiner, M.J. Boone, F.A. Krens, W. Schwab

    Research output: Contribution to journalArticleAcademicpeer-review

    35 Citations (Scopus)

    Abstract

    A complex mixture of hundreds of substances determines strawberry (Fragariaxananassa) aroma, but only 15 volatiles are considered as key flavour compounds. Of these, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is regarded as the most important, but it is methylated further by FaOMT (Fragariaxananassa O-methyltransferase) to 2,5-dimethyl-4-methoxy-3(2H)-furanone (DMMF) during the ripening process. It is shown here that transformation of strawberry with the FaOMT sequence in sense and antisense orientation, under the control of the cauliflower mosaic virus 35S promoter, resulted in a near total loss of DMMF, whereas the levels of the other volatiles remained unchanged. FaOMT repression also affected the ratio of feruloyl 1-O-ß-D-glucose and caffeoyl 1-O-ß-D-glucose, indicating a dual function of the enzyme in planta. Thus, FaOMT is involved in at least two different biochemical pathways in ripe strawberry fruit
    Original languageEnglish
    Pages (from-to)2445-2453
    JournalJournal of Experimental Botany
    Volume57
    Issue number10
    DOIs
    Publication statusPublished - 2006

    Fingerprint

    Fragaria
    Fragaria ananassa
    methyltransferases
    Methyltransferases
    phenylpropanoids
    furaneol
    Up-Regulation
    Down-Regulation
    strawberries
    metabolism
    Caulimovirus
    Glucose
    Complex Mixtures
    glucose
    Cauliflower mosaic virus
    Fruit
    flavor compounds
    Plantae
    biochemical pathways
    ripening

    Keywords

    • strawberry fragaria-ananassa
    • flavor compounds
    • gene-expression
    • tomato fruit
    • molecular-biology
    • dna microarrays
    • messenger-rnas
    • cell-wall
    • identification
    • aroma

    Cite this

    @article{bc28b48364be4e5eb83cf6929269401c,
    title = "Up- and downregulation of Fragaria x ananassa O-methyltransferase: Impacts on Furanone and Phenylpropanoid metabolism",
    abstract = "A complex mixture of hundreds of substances determines strawberry (Fragariaxananassa) aroma, but only 15 volatiles are considered as key flavour compounds. Of these, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is regarded as the most important, but it is methylated further by FaOMT (Fragariaxananassa O-methyltransferase) to 2,5-dimethyl-4-methoxy-3(2H)-furanone (DMMF) during the ripening process. It is shown here that transformation of strawberry with the FaOMT sequence in sense and antisense orientation, under the control of the cauliflower mosaic virus 35S promoter, resulted in a near total loss of DMMF, whereas the levels of the other volatiles remained unchanged. FaOMT repression also affected the ratio of feruloyl 1-O-{\ss}-D-glucose and caffeoyl 1-O-{\ss}-D-glucose, indicating a dual function of the enzyme in planta. Thus, FaOMT is involved in at least two different biochemical pathways in ripe strawberry fruit",
    keywords = "strawberry fragaria-ananassa, flavor compounds, gene-expression, tomato fruit, molecular-biology, dna microarrays, messenger-rnas, cell-wall, identification, aroma",
    author = "S. Lunkenbein and E.M.J. Salentijn and H. Coiner and M.J. Boone and F.A. Krens and W. Schwab",
    note = "accepted for publication",
    year = "2006",
    doi = "10.1093/jxb/erl008",
    language = "English",
    volume = "57",
    pages = "2445--2453",
    journal = "Journal of Experimental Botany",
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    publisher = "Oxford University Press",
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    }

    Up- and downregulation of Fragaria x ananassa O-methyltransferase: Impacts on Furanone and Phenylpropanoid metabolism. / Lunkenbein, S.; Salentijn, E.M.J.; Coiner, H.; Boone, M.J.; Krens, F.A.; Schwab, W.

    In: Journal of Experimental Botany, Vol. 57, No. 10, 2006, p. 2445-2453.

    Research output: Contribution to journalArticleAcademicpeer-review

    TY - JOUR

    T1 - Up- and downregulation of Fragaria x ananassa O-methyltransferase: Impacts on Furanone and Phenylpropanoid metabolism

    AU - Lunkenbein, S.

    AU - Salentijn, E.M.J.

    AU - Coiner, H.

    AU - Boone, M.J.

    AU - Krens, F.A.

    AU - Schwab, W.

    N1 - accepted for publication

    PY - 2006

    Y1 - 2006

    N2 - A complex mixture of hundreds of substances determines strawberry (Fragariaxananassa) aroma, but only 15 volatiles are considered as key flavour compounds. Of these, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is regarded as the most important, but it is methylated further by FaOMT (Fragariaxananassa O-methyltransferase) to 2,5-dimethyl-4-methoxy-3(2H)-furanone (DMMF) during the ripening process. It is shown here that transformation of strawberry with the FaOMT sequence in sense and antisense orientation, under the control of the cauliflower mosaic virus 35S promoter, resulted in a near total loss of DMMF, whereas the levels of the other volatiles remained unchanged. FaOMT repression also affected the ratio of feruloyl 1-O-ß-D-glucose and caffeoyl 1-O-ß-D-glucose, indicating a dual function of the enzyme in planta. Thus, FaOMT is involved in at least two different biochemical pathways in ripe strawberry fruit

    AB - A complex mixture of hundreds of substances determines strawberry (Fragariaxananassa) aroma, but only 15 volatiles are considered as key flavour compounds. Of these, 4-hydroxy-2,5-dimethyl-3(2H)-furanone (HDMF) is regarded as the most important, but it is methylated further by FaOMT (Fragariaxananassa O-methyltransferase) to 2,5-dimethyl-4-methoxy-3(2H)-furanone (DMMF) during the ripening process. It is shown here that transformation of strawberry with the FaOMT sequence in sense and antisense orientation, under the control of the cauliflower mosaic virus 35S promoter, resulted in a near total loss of DMMF, whereas the levels of the other volatiles remained unchanged. FaOMT repression also affected the ratio of feruloyl 1-O-ß-D-glucose and caffeoyl 1-O-ß-D-glucose, indicating a dual function of the enzyme in planta. Thus, FaOMT is involved in at least two different biochemical pathways in ripe strawberry fruit

    KW - strawberry fragaria-ananassa

    KW - flavor compounds

    KW - gene-expression

    KW - tomato fruit

    KW - molecular-biology

    KW - dna microarrays

    KW - messenger-rnas

    KW - cell-wall

    KW - identification

    KW - aroma

    U2 - 10.1093/jxb/erl008

    DO - 10.1093/jxb/erl008

    M3 - Article

    VL - 57

    SP - 2445

    EP - 2453

    JO - Journal of Experimental Botany

    JF - Journal of Experimental Botany

    SN - 0022-0957

    IS - 10

    ER -