Untargeted LC-Q-TOF mass spectrometry method for the detection of adulterations in skimmed-milk powder

J.H.G. Cordewener, D.M.A.M. Luykx, R. Frankhuizen, M.G.E.G. Bremer, H. Hooijerink, A.H.P. America

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44 Citations (Scopus)

Abstract

A nontargeted protein identification method was developed to screen for adulterations in skimmed-milk powder (SMP). There are indications of falsified SMP content due to the addition of plant proteins. To demonstrate the reliability and accuracy of the developed comparative LC-MS method using a quadrupole TOF MS instrument, adulterated SMP samples were prepared by the addition of protein isolates of soy and pea to skimmed-milk before pasteurisation and evaporation. The comparative LC-MS approach enabled unequivocal discrimination of those SMP samples containing soy and pea protein from nonadulterated SMP. To identify the source of (plant) proteins present in the adulterated SMP, data-dependent LC-MS/MS was used in combination with an include list of differential peptides. Numerous peptides originating from the major seed proteins of soy (glycinin, beta-conglycin) and pea (legumin, vicilin) could be identified in this way
Original languageEnglish
Pages (from-to)1216-1223
JournalJournal of Separation Science
Volume32
Issue number8
DOIs
Publication statusPublished - 2009

Keywords

  • quantitative proteomic analysis
  • dairy-products
  • food proteins
  • identification
  • ms

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