Unravelling the mechanism of differential biological responses induced by food-borne xeno- and phyto-estrogenic compounds

A.M. Sotoca Covaleda

Research output: Thesisinternal PhD, WUAcademic

Abstract

The multiple actions of estradiol and other estrogenic compounds in mammalian physiology are brought about, on a molecular level, as a result of complex signalling pathways, and mediated by at least two receptors namely estrogen receptor (ER) α and ERβ.
The aim of the work presented in this thesis was to obtain insight in the role of ERα, ERβ and the ratio of ERα/ERβ present within a cell, in the cellular response to estrogen-like compounds. To this end, this thesis addressed the transcriptional activity at both the gene and protein level and effects on cell proliferation under the influence of specifically-acting estrogen-like molecules when varying the ratio of ERα/ERβ present in the cells under study. The ultimate aim was to link the data on cell proliferation as the biological end-point to the transcriptomics and proteomics data.

LanguageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Rietjens, Ivonne, Promotor
  • Murk, Tinka, Promotor
  • Vervoort, Jacques, Co-promotor
Award date14 Sep 2010
Place of Publication[S.l.
Publisher
Print ISBNs9789085857075
Publication statusPublished - 2010

Fingerprint

Estrogens
Food
Cell Proliferation
Estrogen Receptor alpha
Proteomics
Estradiol
Proteins

Keywords

  • oestrogens
  • plant oestrogens
  • synthetic oestrogens
  • oestrogen receptors
  • gene expression
  • proteomics
  • transcriptomics

Cite this

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title = "Unravelling the mechanism of differential biological responses induced by food-borne xeno- and phyto-estrogenic compounds",
abstract = "The multiple actions of estradiol and other estrogenic compounds in mammalian physiology are brought about, on a molecular level, as a result of complex signalling pathways, and mediated by at least two receptors namely estrogen receptor (ER) α and ERβ. The aim of the work presented in this thesis was to obtain insight in the role of ERα, ERβ and the ratio of ERα/ERβ present within a cell, in the cellular response to estrogen-like compounds. To this end, this thesis addressed the transcriptional activity at both the gene and protein level and effects on cell proliferation under the influence of specifically-acting estrogen-like molecules when varying the ratio of ERα/ERβ present in the cells under study. The ultimate aim was to link the data on cell proliferation as the biological end-point to the transcriptomics and proteomics data.",
keywords = "oestrogenen, plantenoestrogenen, synthetische oestrogenen, oestrogeenreceptoren, genexpressie, eiwitexpressieanalyse, transcriptomics, oestrogens, plant oestrogens, synthetic oestrogens, oestrogen receptors, gene expression, proteomics, transcriptomics",
author = "{Sotoca Covaleda}, A.M.",
note = "WU thesis 4871",
year = "2010",
language = "English",
isbn = "9789085857075",
publisher = "S.n.",
school = "Wageningen University",

}

Sotoca Covaleda, AM 2010, 'Unravelling the mechanism of differential biological responses induced by food-borne xeno- and phyto-estrogenic compounds', Doctor of Philosophy, Wageningen University, [S.l..

Unravelling the mechanism of differential biological responses induced by food-borne xeno- and phyto-estrogenic compounds. / Sotoca Covaleda, A.M.

[S.l. : S.n., 2010. 186 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Unravelling the mechanism of differential biological responses induced by food-borne xeno- and phyto-estrogenic compounds

AU - Sotoca Covaleda, A.M.

N1 - WU thesis 4871

PY - 2010

Y1 - 2010

N2 - The multiple actions of estradiol and other estrogenic compounds in mammalian physiology are brought about, on a molecular level, as a result of complex signalling pathways, and mediated by at least two receptors namely estrogen receptor (ER) α and ERβ. The aim of the work presented in this thesis was to obtain insight in the role of ERα, ERβ and the ratio of ERα/ERβ present within a cell, in the cellular response to estrogen-like compounds. To this end, this thesis addressed the transcriptional activity at both the gene and protein level and effects on cell proliferation under the influence of specifically-acting estrogen-like molecules when varying the ratio of ERα/ERβ present in the cells under study. The ultimate aim was to link the data on cell proliferation as the biological end-point to the transcriptomics and proteomics data.

AB - The multiple actions of estradiol and other estrogenic compounds in mammalian physiology are brought about, on a molecular level, as a result of complex signalling pathways, and mediated by at least two receptors namely estrogen receptor (ER) α and ERβ. The aim of the work presented in this thesis was to obtain insight in the role of ERα, ERβ and the ratio of ERα/ERβ present within a cell, in the cellular response to estrogen-like compounds. To this end, this thesis addressed the transcriptional activity at both the gene and protein level and effects on cell proliferation under the influence of specifically-acting estrogen-like molecules when varying the ratio of ERα/ERβ present in the cells under study. The ultimate aim was to link the data on cell proliferation as the biological end-point to the transcriptomics and proteomics data.

KW - oestrogenen

KW - plantenoestrogenen

KW - synthetische oestrogenen

KW - oestrogeenreceptoren

KW - genexpressie

KW - eiwitexpressieanalyse

KW - transcriptomics

KW - oestrogens

KW - plant oestrogens

KW - synthetic oestrogens

KW - oestrogen receptors

KW - gene expression

KW - proteomics

KW - transcriptomics

M3 - internal PhD, WU

SN - 9789085857075

PB - S.n.

CY - [S.l.

ER -