Unravelling the mechanism of differential biological responses induced by food-borne xeno- and phyto-estrogenic compounds

A.M. Sotoca Covaleda

Research output: Thesisinternal PhD, WU

Abstract

The multiple actions of estradiol and other estrogenic compounds in mammalian physiology are brought about, on a molecular level, as a result of complex signalling pathways, and mediated by at least two receptors namely estrogen receptor (ER) α and ERβ.
The aim of the work presented in this thesis was to obtain insight in the role of ERα, ERβ and the ratio of ERα/ERβ present within a cell, in the cellular response to estrogen-like compounds. To this end, this thesis addressed the transcriptional activity at both the gene and protein level and effects on cell proliferation under the influence of specifically-acting estrogen-like molecules when varying the ratio of ERα/ERβ present in the cells under study. The ultimate aim was to link the data on cell proliferation as the biological end-point to the transcriptomics and proteomics data.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Rietjens, Ivonne, Promotor
  • Murk, Tinka, Promotor
  • Vervoort, Jacques, Co-promotor
Award date14 Sep 2010
Place of Publication[S.l.
Publisher
Print ISBNs9789085857075
Publication statusPublished - 2010

Keywords

  • oestrogens
  • plant oestrogens
  • synthetic oestrogens
  • oestrogen receptors
  • gene expression
  • proteomics
  • transcriptomics

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