Abstract
High-throughput technologies are urgently needed for monitoring the formidable biodiversity and functional capabilities of microorganisms in the environment. Ten years ago, DNA microarrays, miniaturized platforms for highly parallel hybridization reactions, found their way into environmental microbiology and raised great expectations among researchers in the field. In this article, we briefly summarize the state-of-the-art of microarray approaches in microbial ecology research and discuss in more detail crucial problems and promising solutions. Finally, we outline scenarios for an innovative combination of microarrays with other molecular tools for structure-function analysis of complex microbial communities.
| Original language | English |
|---|---|
| Pages (from-to) | 498-506 |
| Journal | Microbial Ecology |
| Volume | 53 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 2007 |
Keywords
- nonequilibrium dissociation approach
- sulfate-reducing prokaryotes
- in-situ hybridization
- ribosomal-rna
- oligonucleotide microarray
- uncultured microorganisms
- marine bacterioplankton
- diagnostic microarray
- parallel detection
- gene-expression
