Unraveling the Entry Mechanism of Baculoviruses and Its Evolutionary Implications

M. Wang, J. Wang, F. Yin, Y. Tan, F. Deng, X. Chen, J.A. Jehle, J.M. Vlak, Zhihong Hu, H. Wang

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

The entry of baculovirus budded virus into host cells is mediated by two distinct types of envelope fusion proteins (EFPs), GP64 and F protein. Phylogenetic analysis suggested that F proteins were ancestral baculovirus EFPs, whereas GP64 was acquired by progenitor group I alphabaculovirus more recently and may have stimulated the formation of the group I lineage. This study was designed to experimentally recapitulate a possible major step in the evolution of baculoviruses. We demonstrated that the infectivity of an F-null group II alphabaculovirus (Helicoverpa armigera nucleopolyhedrovirus [HearNPV]) can be functionally rescued by coinsertion of GP64 along with the nonfusogenic Fdef (furin site mutated HaF) from HearNPV. Interestingly, HearNPV enters cells by endocytosis and, less efficiently, by direct membrane fusion at low pH. However, this recombinant HearNPV coexpressing Fdef and GP64 mimicked group I virus not only in its EFP composition but also in its abilities to enter host cells via low-pH-triggered direct fusion pathway. Neutralization assays indicated that the nonfusogenic F proteins contribute mainly to binding to susceptible cells, while GP64 contributes to fusion. Coinsertion of GP64 with an F-like protein (Ac23) from group I virus led to efficient rescue of an F-null group II virus. In summary, these recombinant viruses and their entry modes are considered to resemble an evolutionary event of the acquisition of GP64 by an ancestral group I virus and subsequent adaptive inactivation of the original F protein. The study described here provides the first experimental evidence to support the hypothesis of the evolution of baculovirus EFPs.
Original languageEnglish
Pages (from-to)2301-2311
JournalJournal of Virology
Volume88
Issue number4
DOIs
Publication statusPublished - 2014

Keywords

  • envelope fusion protein
  • californica multicapsid nucleopolyhedrovirus
  • nuclear polyhedrosis-virus
  • mammalian-cells
  • f-proteins
  • gp64
  • infectivity
  • insect
  • identification
  • neutralization

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