Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways

K.C.M. Verhoeckx; H.A.A.J. Korthout; A.P. van Meeteren-Kreikamp; K.A. Ehlert; M. Wang; J. de Greef; R.J.T. Rodenburg; R.F. Witkamp

doi:10.1016/j.intimp.2005.10.002

Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways

K.C.M. Verhoeckx, H.A.A.J. Korthout, A.P. van Meeteren-Kreikamp, K.A. Ehlert, M. Wang, J. de Greef, R.J.T. Rodenburg, R.F. Witkamp

Research output: Contribution to journal › Article › Academic › peer-review

69 Citations (Scopus)

Abstract

There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of ¿9-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent ¿9-tetrahydrocanabinoid acid (THCa). By heating Cannabis extracts, THCa was shown to be converted into THC. Unheated Cannabis extract and THCa were able to inhibit the tumor necrosis factor alpha (TNF-a) levels in culture supernatants from U937 macrophages and peripheral blood macrophages after stimulation with LPS in a dose-dependent manner. This inhibition persisted over a longer period of time, whereas after prolonged exposure time THC and heated Cannabis extract tend to induce the TNF-a level. Furthermore we demonstrated that THCa and THC show distinct effects on phosphatidylcholine specific phospholipase C (PC-PLC) activity. Unheated Cannabis extract and THCa inhibit the PC-PLC activity in a dose-dependent manner, while THC induced PC-PLC activity at high concentrations. These results suggest that THCa and THC exert their immuno-modulating effects via different metabolic pathways

Original language	English
Pages (from-to)	656-665
Journal	International Immunopharmacology
Volume	6
Issue number	4
DOIs	https://doi.org/10.1016/j.intimp.2005.10.002
Publication status	Published - 2006

Keywords

human alveolar macrophages
phospholipase-c
cell-line
kappa-b
cytokine
lipopolysaccharide
activation
brain
transcriptomics
differentiation

Access to Document

10.1016/j.intimp.2005.10.002

Cite this

Verhoeckx, K. C. M., Korthout, H. A. A. J., van Meeteren-Kreikamp, A. P., Ehlert, K. A., Wang, M., de Greef, J., Rodenburg, R. J. T., & Witkamp, R. F. (2006). Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways. International Immunopharmacology, 6(4), 656-665. https://doi.org/10.1016/j.intimp.2005.10.002

@article{6d718070da894a0ba1a13745d7e9ad53,

title = "Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways",

abstract = "There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of ¿9-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent ¿9-tetrahydrocanabinoid acid (THCa). By heating Cannabis extracts, THCa was shown to be converted into THC. Unheated Cannabis extract and THCa were able to inhibit the tumor necrosis factor alpha (TNF-a) levels in culture supernatants from U937 macrophages and peripheral blood macrophages after stimulation with LPS in a dose-dependent manner. This inhibition persisted over a longer period of time, whereas after prolonged exposure time THC and heated Cannabis extract tend to induce the TNF-a level. Furthermore we demonstrated that THCa and THC show distinct effects on phosphatidylcholine specific phospholipase C (PC-PLC) activity. Unheated Cannabis extract and THCa inhibit the PC-PLC activity in a dose-dependent manner, while THC induced PC-PLC activity at high concentrations. These results suggest that THCa and THC exert their immuno-modulating effects via different metabolic pathways",

keywords = "human alveolar macrophages, phospholipase-c, cell-line, kappa-b, cytokine, lipopolysaccharide, activation, brain, transcriptomics, differentiation",

author = "K.C.M. Verhoeckx and H.A.A.J. Korthout and {van Meeteren-Kreikamp}, A.P. and K.A. Ehlert and M. Wang and {de Greef}, J. and R.J.T. Rodenburg and R.F. Witkamp",

year = "2006",

doi = "10.1016/j.intimp.2005.10.002",

language = "English",

volume = "6",

pages = "656--665",

journal = "International Immunopharmacology",

issn = "1567-5769",

publisher = "Elsevier",

number = "4",

}

Verhoeckx, KCM, Korthout, HAAJ, van Meeteren-Kreikamp, AP, Ehlert, KA, Wang, M, de Greef, J, Rodenburg, RJT & Witkamp, RF 2006, 'Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways', International Immunopharmacology, vol. 6, no. 4, pp. 656-665. https://doi.org/10.1016/j.intimp.2005.10.002

Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways. / Verhoeckx, K.C.M.; Korthout, H.A.A.J.; van Meeteren-Kreikamp, A.P. et al.
In: International Immunopharmacology, Vol. 6, No. 4, 2006, p. 656-665.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways

AU - Verhoeckx, K.C.M.

AU - Korthout, H.A.A.J.

AU - van Meeteren-Kreikamp, A.P.

AU - Ehlert, K.A.

AU - Wang, M.

AU - de Greef, J.

AU - Rodenburg, R.J.T.

AU - Witkamp, R.F.

PY - 2006

Y1 - 2006

N2 - There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of ¿9-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent ¿9-tetrahydrocanabinoid acid (THCa). By heating Cannabis extracts, THCa was shown to be converted into THC. Unheated Cannabis extract and THCa were able to inhibit the tumor necrosis factor alpha (TNF-a) levels in culture supernatants from U937 macrophages and peripheral blood macrophages after stimulation with LPS in a dose-dependent manner. This inhibition persisted over a longer period of time, whereas after prolonged exposure time THC and heated Cannabis extract tend to induce the TNF-a level. Furthermore we demonstrated that THCa and THC show distinct effects on phosphatidylcholine specific phospholipase C (PC-PLC) activity. Unheated Cannabis extract and THCa inhibit the PC-PLC activity in a dose-dependent manner, while THC induced PC-PLC activity at high concentrations. These results suggest that THCa and THC exert their immuno-modulating effects via different metabolic pathways

AB - There is a great interest in the pharmacological properties of cannabinoid like compounds that are not linked to the adverse effects of ¿9-tetrahydrocannabinol (THC), e.g. psychoactive properties. The present paper describes the potential immuno-modulating activity of unheated Cannabis sativa extracts and its main non-psychoactive constituent ¿9-tetrahydrocanabinoid acid (THCa). By heating Cannabis extracts, THCa was shown to be converted into THC. Unheated Cannabis extract and THCa were able to inhibit the tumor necrosis factor alpha (TNF-a) levels in culture supernatants from U937 macrophages and peripheral blood macrophages after stimulation with LPS in a dose-dependent manner. This inhibition persisted over a longer period of time, whereas after prolonged exposure time THC and heated Cannabis extract tend to induce the TNF-a level. Furthermore we demonstrated that THCa and THC show distinct effects on phosphatidylcholine specific phospholipase C (PC-PLC) activity. Unheated Cannabis extract and THCa inhibit the PC-PLC activity in a dose-dependent manner, while THC induced PC-PLC activity at high concentrations. These results suggest that THCa and THC exert their immuno-modulating effects via different metabolic pathways

KW - human alveolar macrophages

KW - phospholipase-c

KW - cell-line

KW - kappa-b

KW - cytokine

KW - lipopolysaccharide

KW - activation

KW - brain

KW - transcriptomics

KW - differentiation

U2 - 10.1016/j.intimp.2005.10.002

DO - 10.1016/j.intimp.2005.10.002

M3 - Article

SN - 1567-5769

VL - 6

SP - 656

EP - 665

JO - International Immunopharmacology

JF - International Immunopharmacology

IS - 4

ER -

Unheated Cannabis sativa extracts and its major compound THC-acid have potential immuno-modulating properties not mediated bu CB1 en CB2 receptor coupled pathways

Abstract

Keywords

Access to Document

Fingerprint

Cite this