Ultraviolet radiation enhances salicylic acid-mediated defense signaling and resistance to Pseudomonas syringae DC3000 in a jasmonic acid-deficient tomato mutant

Rocío Escobar Bravo*, Gang Chen, Katharina Grosser, Nicole M. Van Dam, Kirsten A. Leiss, Peter G.L. Klinkhamer

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Ultraviolet radiation (UV) is an important modulator of plant defenses against biotic stresses. We have recently described that different supplemental UV exposure times and irradiance intensities enhanced tomato (Solanum lycopersicum) resistance to Western flower thrips (Frankliniella occidentalis). UV increased jasmonic acid-isoleucine (JA-Ile) and salicylic acid (SA) levels, as well as the expression of JA- and SA-responsive genes, before thrips herbivory. Here we report how UV affects tomato defense responses upon thrips infestation, and resistance to pathogens that are susceptible to the activation of SA-associated defenses. Our experiments reveal that, at 7 days after thrips infestation, UV did not enhance the levels of jasmonates, auxin or abscisic acid. UV also did not affect the expression of JA-responsive genes in the cultivar Moneymaker, the jasmonate deficient mutant def-1, the type-VI trichome deficient mutant od-2, or their wild-type Castlemart. However, UV strongly activated SA-associated defense responses in def-1 after thrips infestation. Further bioassays showed that UV increased def-1 resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000, which is susceptible to SA-mediated defenses. Our results suggest that UV might enhance tomato resistance to this pathogen in the JA deficient genotype through the activation of SA defenses.

Original languageEnglish
Pages (from-to)e1581560
JournalPlant Signaling & Behavior
Volume14
Issue number4
DOIs
Publication statusPublished - 20 Feb 2019

Fingerprint

Pseudomonas syringae
jasmonic acid
salicylic acid
ultraviolet radiation
tomatoes
mutants
Thysanoptera
Frankliniella occidentalis
pathogens
Pseudomonas syringae pv. tomato
Solanum lycopersicum
isoleucine
biotic stress
trichomes
exposure duration
abscisic acid
auxins
herbivores
genes
bioassays

Keywords

  • Abscisic acid
  • auxin
  • jasmonates
  • light
  • salicylic acid
  • ultraviolet radiation
  • western flower thrips

Cite this

Escobar Bravo, Rocío ; Chen, Gang ; Grosser, Katharina ; Van Dam, Nicole M. ; Leiss, Kirsten A. ; Klinkhamer, Peter G.L. / Ultraviolet radiation enhances salicylic acid-mediated defense signaling and resistance to Pseudomonas syringae DC3000 in a jasmonic acid-deficient tomato mutant. In: Plant Signaling & Behavior. 2019 ; Vol. 14, No. 4. pp. e1581560.
@article{3c73fc2930da403185a112e03de7ba3b,
title = "Ultraviolet radiation enhances salicylic acid-mediated defense signaling and resistance to Pseudomonas syringae DC3000 in a jasmonic acid-deficient tomato mutant",
abstract = "Ultraviolet radiation (UV) is an important modulator of plant defenses against biotic stresses. We have recently described that different supplemental UV exposure times and irradiance intensities enhanced tomato (Solanum lycopersicum) resistance to Western flower thrips (Frankliniella occidentalis). UV increased jasmonic acid-isoleucine (JA-Ile) and salicylic acid (SA) levels, as well as the expression of JA- and SA-responsive genes, before thrips herbivory. Here we report how UV affects tomato defense responses upon thrips infestation, and resistance to pathogens that are susceptible to the activation of SA-associated defenses. Our experiments reveal that, at 7 days after thrips infestation, UV did not enhance the levels of jasmonates, auxin or abscisic acid. UV also did not affect the expression of JA-responsive genes in the cultivar Moneymaker, the jasmonate deficient mutant def-1, the type-VI trichome deficient mutant od-2, or their wild-type Castlemart. However, UV strongly activated SA-associated defense responses in def-1 after thrips infestation. Further bioassays showed that UV increased def-1 resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000, which is susceptible to SA-mediated defenses. Our results suggest that UV might enhance tomato resistance to this pathogen in the JA deficient genotype through the activation of SA defenses.",
keywords = "Abscisic acid, auxin, jasmonates, light, salicylic acid, ultraviolet radiation, western flower thrips",
author = "{Escobar Bravo}, Roc{\'i}o and Gang Chen and Katharina Grosser and {Van Dam}, {Nicole M.} and Leiss, {Kirsten A.} and Klinkhamer, {Peter G.L.}",
year = "2019",
month = "2",
day = "20",
doi = "10.1080/15592324.2019.1581560",
language = "English",
volume = "14",
pages = "e1581560",
journal = "Plant Signaling & Behavior",
issn = "1559-2316",
publisher = "Taylor & Francis",
number = "4",

}

Ultraviolet radiation enhances salicylic acid-mediated defense signaling and resistance to Pseudomonas syringae DC3000 in a jasmonic acid-deficient tomato mutant. / Escobar Bravo, Rocío; Chen, Gang; Grosser, Katharina; Van Dam, Nicole M.; Leiss, Kirsten A.; Klinkhamer, Peter G.L.

In: Plant Signaling & Behavior, Vol. 14, No. 4, 20.02.2019, p. e1581560.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Ultraviolet radiation enhances salicylic acid-mediated defense signaling and resistance to Pseudomonas syringae DC3000 in a jasmonic acid-deficient tomato mutant

AU - Escobar Bravo, Rocío

AU - Chen, Gang

AU - Grosser, Katharina

AU - Van Dam, Nicole M.

AU - Leiss, Kirsten A.

AU - Klinkhamer, Peter G.L.

PY - 2019/2/20

Y1 - 2019/2/20

N2 - Ultraviolet radiation (UV) is an important modulator of plant defenses against biotic stresses. We have recently described that different supplemental UV exposure times and irradiance intensities enhanced tomato (Solanum lycopersicum) resistance to Western flower thrips (Frankliniella occidentalis). UV increased jasmonic acid-isoleucine (JA-Ile) and salicylic acid (SA) levels, as well as the expression of JA- and SA-responsive genes, before thrips herbivory. Here we report how UV affects tomato defense responses upon thrips infestation, and resistance to pathogens that are susceptible to the activation of SA-associated defenses. Our experiments reveal that, at 7 days after thrips infestation, UV did not enhance the levels of jasmonates, auxin or abscisic acid. UV also did not affect the expression of JA-responsive genes in the cultivar Moneymaker, the jasmonate deficient mutant def-1, the type-VI trichome deficient mutant od-2, or their wild-type Castlemart. However, UV strongly activated SA-associated defense responses in def-1 after thrips infestation. Further bioassays showed that UV increased def-1 resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000, which is susceptible to SA-mediated defenses. Our results suggest that UV might enhance tomato resistance to this pathogen in the JA deficient genotype through the activation of SA defenses.

AB - Ultraviolet radiation (UV) is an important modulator of plant defenses against biotic stresses. We have recently described that different supplemental UV exposure times and irradiance intensities enhanced tomato (Solanum lycopersicum) resistance to Western flower thrips (Frankliniella occidentalis). UV increased jasmonic acid-isoleucine (JA-Ile) and salicylic acid (SA) levels, as well as the expression of JA- and SA-responsive genes, before thrips herbivory. Here we report how UV affects tomato defense responses upon thrips infestation, and resistance to pathogens that are susceptible to the activation of SA-associated defenses. Our experiments reveal that, at 7 days after thrips infestation, UV did not enhance the levels of jasmonates, auxin or abscisic acid. UV also did not affect the expression of JA-responsive genes in the cultivar Moneymaker, the jasmonate deficient mutant def-1, the type-VI trichome deficient mutant od-2, or their wild-type Castlemart. However, UV strongly activated SA-associated defense responses in def-1 after thrips infestation. Further bioassays showed that UV increased def-1 resistance to the hemi-biotrophic bacterial pathogen Pseudomonas syringae pv. tomato DC3000, which is susceptible to SA-mediated defenses. Our results suggest that UV might enhance tomato resistance to this pathogen in the JA deficient genotype through the activation of SA defenses.

KW - Abscisic acid

KW - auxin

KW - jasmonates

KW - light

KW - salicylic acid

KW - ultraviolet radiation

KW - western flower thrips

U2 - 10.1080/15592324.2019.1581560

DO - 10.1080/15592324.2019.1581560

M3 - Article

VL - 14

SP - e1581560

JO - Plant Signaling & Behavior

JF - Plant Signaling & Behavior

SN - 1559-2316

IS - 4

ER -