Ultratraces of carotenes in tomato purees : HPLC-TLS study

S. Luterotti, K. Markovic, M. Franko, D.D. Bicanic, N. Vahcic, O. Doka

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

The present study was designed to provide information about (i) the profile of carotene pigments and (ii) trace quantities of lycopene and -carotene left in tomato purées. The ultrasensitive method comprising HPLC and thermal lens spectrometric (TLS) detection enabled us to detect as low as 0.3 and 1.1 ng ml¿1 lycopene and -carotene in purée extracts, respectively. Total concentration of -carotene and lycopene (varying from 3 to 170 ng g¿1) in the examined tomato purées may serve as an indicator of the carotene-specific antioxidative capacity of these products. Although conventional spectrophotometry can be used to rapidly assess the quality of products derived from tomatoes, a highly sensitive and selective method such as HPLC-TLS is needed for reliable analyses of samples such as, for example, those subjected to inappropriate storage and/or handling
Original languageEnglish
Pages (from-to)684-686
JournalReview of Scientific Instruments
Volume74
Issue number1
DOIs
Publication statusPublished - 2003

Keywords

  • performance liquid-chromatography
  • thermal lens detection
  • trans-beta-carotene
  • ultrasensitive determination
  • lycopene
  • isomers
  • oxygen

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