Ultratraces of carotenes in tomato purees : HPLC-TLS study

S. Luterotti; K. Markovic; M. Franko; D.D. Bicanic; N. Vahcic; O. Doka

doi:10.1063/1.1512773

Ultratraces of carotenes in tomato purees : HPLC-TLS study

S. Luterotti, K. Markovic, M. Franko, D.D. Bicanic, N. Vahcic, O. Doka

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

The present study was designed to provide information about (i) the profile of carotene pigments and (ii) trace quantities of lycopene and -carotene left in tomato purées. The ultrasensitive method comprising HPLC and thermal lens spectrometric (TLS) detection enabled us to detect as low as 0.3 and 1.1 ng ml¿1 lycopene and -carotene in purée extracts, respectively. Total concentration of -carotene and lycopene (varying from 3 to 170 ng g¿1) in the examined tomato purées may serve as an indicator of the carotene-specific antioxidative capacity of these products. Although conventional spectrophotometry can be used to rapidly assess the quality of products derived from tomatoes, a highly sensitive and selective method such as HPLC-TLS is needed for reliable analyses of samples such as, for example, those subjected to inappropriate storage and/or handling

Original language	English
Pages (from-to)	684-686
Journal	Review of Scientific Instruments
Volume	74
Issue number	1
DOIs	https://doi.org/10.1063/1.1512773
Publication status	Published - 2003

Keywords

performance liquid-chromatography
thermal lens detection
trans-beta-carotene
ultrasensitive determination
lycopene
isomers
oxygen

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10.1063/1.1512773

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title = "Ultratraces of carotenes in tomato purees : HPLC-TLS study",

abstract = "The present study was designed to provide information about (i) the profile of carotene pigments and (ii) trace quantities of lycopene and -carotene left in tomato pur{\'e}es. The ultrasensitive method comprising HPLC and thermal lens spectrometric (TLS) detection enabled us to detect as low as 0.3 and 1.1 ng ml¿1 lycopene and -carotene in pur{\'e}e extracts, respectively. Total concentration of -carotene and lycopene (varying from 3 to 170 ng g¿1) in the examined tomato pur{\'e}es may serve as an indicator of the carotene-specific antioxidative capacity of these products. Although conventional spectrophotometry can be used to rapidly assess the quality of products derived from tomatoes, a highly sensitive and selective method such as HPLC-TLS is needed for reliable analyses of samples such as, for example, those subjected to inappropriate storage and/or handling",

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doi = "10.1063/1.1512773",

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T1 - Ultratraces of carotenes in tomato purees : HPLC-TLS study

AU - Luterotti, S.

AU - Markovic, K.

AU - Franko, M.

AU - Bicanic, D.D.

AU - Vahcic, N.

AU - Doka, O.

PY - 2003

Y1 - 2003

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AB - The present study was designed to provide information about (i) the profile of carotene pigments and (ii) trace quantities of lycopene and -carotene left in tomato purées. The ultrasensitive method comprising HPLC and thermal lens spectrometric (TLS) detection enabled us to detect as low as 0.3 and 1.1 ng ml¿1 lycopene and -carotene in purée extracts, respectively. Total concentration of -carotene and lycopene (varying from 3 to 170 ng g¿1) in the examined tomato purées may serve as an indicator of the carotene-specific antioxidative capacity of these products. Although conventional spectrophotometry can be used to rapidly assess the quality of products derived from tomatoes, a highly sensitive and selective method such as HPLC-TLS is needed for reliable analyses of samples such as, for example, those subjected to inappropriate storage and/or handling

KW - performance liquid-chromatography

KW - thermal lens detection

KW - trans-beta-carotene

KW - ultrasensitive determination

KW - lycopene

KW - isomers

KW - oxygen

U2 - 10.1063/1.1512773

DO - 10.1063/1.1512773

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JF - Review of Scientific Instruments

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Ultratraces of carotenes in tomato purees : HPLC-TLS study

Abstract

Keywords

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