Ultratrace LC-MS/MS Analysis of Segmented Calf Hair for Retrospective Assessment of Time of Clenbuterol Administration in Agriforensics

W.F. Duvivier, T.A. van Beek, T. Meijer, R.J.P. Peeters, M.J. Groot, S.S. Sterk, M.W.F. Nielen

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

In agriforensics, time of administration is often debated when illegal drug residues, such as clenbuterol, are found in frequently traded cattle. In this proof-of-concept work, the feasibility of obtaining retrospective timeline information from segmented calf tail hair analyses has been studied. First, an ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) hair analysis method was adapted to accommodate smaller sample sizes and in-house validated. Then, longitudinal 1 cm segments of calf tail hair were analyzed to obtain clenbuterol concentration profiles. The profiles found were in good agreement with calculated, theoretical positions of the clenbuterol residues along the hair. Following assessment of the average growth rate of calf tail hair, time of clenbuterol administration could be retrospectively determined from segmented hair analysis data. The data from the initial animal treatment study (n = 2) suggest that time of treatment can be retrospectively estimated with an error of 3–17 days.
Original languageEnglish
Pages (from-to)493-499
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number2
DOIs
Publication statusPublished - 2015

Fingerprint

Clenbuterol
clenbuterol
Hair
hairs
calves
Tail
tail
Drug Residues
Mass spectrometry
drug residues
Animals
Sample Size
tandem mass spectrometry
Mass Spectrometry
data analysis
Liquids
mass spectrometry
liquids
cattle
Growth

Keywords

  • tandem mass-spectrometry
  • performance liquid-chromatography
  • beta-agonist residues
  • equine hair
  • bovine hair
  • human urine
  • cattle
  • calves
  • contamination
  • samples

Cite this

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title = "Ultratrace LC-MS/MS Analysis of Segmented Calf Hair for Retrospective Assessment of Time of Clenbuterol Administration in Agriforensics",
abstract = "In agriforensics, time of administration is often debated when illegal drug residues, such as clenbuterol, are found in frequently traded cattle. In this proof-of-concept work, the feasibility of obtaining retrospective timeline information from segmented calf tail hair analyses has been studied. First, an ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) hair analysis method was adapted to accommodate smaller sample sizes and in-house validated. Then, longitudinal 1 cm segments of calf tail hair were analyzed to obtain clenbuterol concentration profiles. The profiles found were in good agreement with calculated, theoretical positions of the clenbuterol residues along the hair. Following assessment of the average growth rate of calf tail hair, time of clenbuterol administration could be retrospectively determined from segmented hair analysis data. The data from the initial animal treatment study (n = 2) suggest that time of treatment can be retrospectively estimated with an error of 3–17 days.",
keywords = "tandem mass-spectrometry, performance liquid-chromatography, beta-agonist residues, equine hair, bovine hair, human urine, cattle, calves, contamination, samples",
author = "W.F. Duvivier and {van Beek}, T.A. and T. Meijer and R.J.P. Peeters and M.J. Groot and S.S. Sterk and M.W.F. Nielen",
year = "2015",
doi = "10.1021/jf5056437",
language = "English",
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pages = "493--499",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
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Ultratrace LC-MS/MS Analysis of Segmented Calf Hair for Retrospective Assessment of Time of Clenbuterol Administration in Agriforensics. / Duvivier, W.F.; van Beek, T.A.; Meijer, T.; Peeters, R.J.P.; Groot, M.J.; Sterk, S.S.; Nielen, M.W.F.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 2, 2015, p. 493-499.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Ultratrace LC-MS/MS Analysis of Segmented Calf Hair for Retrospective Assessment of Time of Clenbuterol Administration in Agriforensics

AU - Duvivier, W.F.

AU - van Beek, T.A.

AU - Meijer, T.

AU - Peeters, R.J.P.

AU - Groot, M.J.

AU - Sterk, S.S.

AU - Nielen, M.W.F.

PY - 2015

Y1 - 2015

N2 - In agriforensics, time of administration is often debated when illegal drug residues, such as clenbuterol, are found in frequently traded cattle. In this proof-of-concept work, the feasibility of obtaining retrospective timeline information from segmented calf tail hair analyses has been studied. First, an ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) hair analysis method was adapted to accommodate smaller sample sizes and in-house validated. Then, longitudinal 1 cm segments of calf tail hair were analyzed to obtain clenbuterol concentration profiles. The profiles found were in good agreement with calculated, theoretical positions of the clenbuterol residues along the hair. Following assessment of the average growth rate of calf tail hair, time of clenbuterol administration could be retrospectively determined from segmented hair analysis data. The data from the initial animal treatment study (n = 2) suggest that time of treatment can be retrospectively estimated with an error of 3–17 days.

AB - In agriforensics, time of administration is often debated when illegal drug residues, such as clenbuterol, are found in frequently traded cattle. In this proof-of-concept work, the feasibility of obtaining retrospective timeline information from segmented calf tail hair analyses has been studied. First, an ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) hair analysis method was adapted to accommodate smaller sample sizes and in-house validated. Then, longitudinal 1 cm segments of calf tail hair were analyzed to obtain clenbuterol concentration profiles. The profiles found were in good agreement with calculated, theoretical positions of the clenbuterol residues along the hair. Following assessment of the average growth rate of calf tail hair, time of clenbuterol administration could be retrospectively determined from segmented hair analysis data. The data from the initial animal treatment study (n = 2) suggest that time of treatment can be retrospectively estimated with an error of 3–17 days.

KW - tandem mass-spectrometry

KW - performance liquid-chromatography

KW - beta-agonist residues

KW - equine hair

KW - bovine hair

KW - human urine

KW - cattle

KW - calves

KW - contamination

KW - samples

U2 - 10.1021/jf5056437

DO - 10.1021/jf5056437

M3 - Article

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EP - 499

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

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