Ubiquitous presence of piscidin-1 in Atlantic cod as evidenced by immunolocalisation

J. Ruangsri, J.M.O. Fernandes, J.H.W.M. Rombout, M. Brinchmann, V. Kiron

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Background: Antimicrobial peptides (AMPs), the natural antibiotics bestowed upon all forms of life, consist of small molecular weight proteins with a broad spectrum antimicrobial activity against a variety of pathogenic microorganisms. Piscidins are one of the AMP families that are imperative for the innate defence mechanisms of teleosts. Atlantic cod, a basal fish belonging to the superorder Paracanthopterygii also possesses multiple piscidin peptides. Two piscidin paralogues (pis1 and pis2) and a novel alternative splice variant of pis2 of this fish were previously described by us. To shed light on other potent roles of these molecules, now we have mapped the distribution of piscidin 1 (Pis1), in different tissues and organs of cod through immunohistochemistry (IHC) employing an affinity purified polyclonal antibody specific to Pis1. Results: Various cell types and tissues of Atlantic cod including those from the immune organs of naive fish are armed with Pis1 peptide. Different types of the blood leucocytes and phagocytic cells among the leucocytes examined gave a relatively strong indication of Pis1 immunopositivity. In addition, other cell types such as hematopoietic cells, epithelial cells and multi-granular cells located in the mucosal and hematopoietic tissues were also Pis1-immunoreactive. More interestingly, chondrocytes appear to produce Pis1 and this is the first report on the presence of an AMP in cartilage tissue of fish. Furthermore, Pis1 immunopositivity was detected in other tissues and organs of naive fish including neural tissues, exocrine and endocrine glands, compound gland cells, excretory kidney, intestinal and respiratory epithelial cells, swim bladder, skin and hypodermis layer, myosepta, liver, heart, eye and oocytes. Conclusions: Pis1 peptide is produced by various cell types located in different tissues and organs of Atlantic cod. It is present in all immune-related organs of naive fish and the elevated peptide expression following phagocytosis strongly suggest their involvement in innate defence. Further, its widespread occurrence in non-immune tissues and organs of apparently healthy fish implies that piscidin may have other functions in addition to its role as an immune effector molecule.
Original languageEnglish
Article number46
JournalBMC Veterinary Research
Volume8
DOIs
Publication statusPublished - 2012

Fingerprint

Gadus morhua
Fishes
Peptides
fish
antimicrobial peptides
peptides
cells
leukocytes
epithelial cells
exocrine glands
Leukocytes
endocrine glands
Epithelial Cells
Exocrine Glands
swim bladder
Endocrine Glands
chondrocytes
Gadiformes
phagocytes
blood groups

Keywords

  • gadus-morhua l.
  • antimicrobial peptides
  • sea-bass
  • dicentrarchus-labrax
  • vibrio-anguillarum
  • immune-systems
  • mast-cells
  • fish
  • expression
  • crosstalk

Cite this

Ruangsri, J., Fernandes, J. M. O., Rombout, J. H. W. M., Brinchmann, M., & Kiron, V. (2012). Ubiquitous presence of piscidin-1 in Atlantic cod as evidenced by immunolocalisation. BMC Veterinary Research, 8, [46]. https://doi.org/10.1186/1746-6148-8-46
Ruangsri, J. ; Fernandes, J.M.O. ; Rombout, J.H.W.M. ; Brinchmann, M. ; Kiron, V. / Ubiquitous presence of piscidin-1 in Atlantic cod as evidenced by immunolocalisation. In: BMC Veterinary Research. 2012 ; Vol. 8.
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abstract = "Background: Antimicrobial peptides (AMPs), the natural antibiotics bestowed upon all forms of life, consist of small molecular weight proteins with a broad spectrum antimicrobial activity against a variety of pathogenic microorganisms. Piscidins are one of the AMP families that are imperative for the innate defence mechanisms of teleosts. Atlantic cod, a basal fish belonging to the superorder Paracanthopterygii also possesses multiple piscidin peptides. Two piscidin paralogues (pis1 and pis2) and a novel alternative splice variant of pis2 of this fish were previously described by us. To shed light on other potent roles of these molecules, now we have mapped the distribution of piscidin 1 (Pis1), in different tissues and organs of cod through immunohistochemistry (IHC) employing an affinity purified polyclonal antibody specific to Pis1. Results: Various cell types and tissues of Atlantic cod including those from the immune organs of naive fish are armed with Pis1 peptide. Different types of the blood leucocytes and phagocytic cells among the leucocytes examined gave a relatively strong indication of Pis1 immunopositivity. In addition, other cell types such as hematopoietic cells, epithelial cells and multi-granular cells located in the mucosal and hematopoietic tissues were also Pis1-immunoreactive. More interestingly, chondrocytes appear to produce Pis1 and this is the first report on the presence of an AMP in cartilage tissue of fish. Furthermore, Pis1 immunopositivity was detected in other tissues and organs of naive fish including neural tissues, exocrine and endocrine glands, compound gland cells, excretory kidney, intestinal and respiratory epithelial cells, swim bladder, skin and hypodermis layer, myosepta, liver, heart, eye and oocytes. Conclusions: Pis1 peptide is produced by various cell types located in different tissues and organs of Atlantic cod. It is present in all immune-related organs of naive fish and the elevated peptide expression following phagocytosis strongly suggest their involvement in innate defence. Further, its widespread occurrence in non-immune tissues and organs of apparently healthy fish implies that piscidin may have other functions in addition to its role as an immune effector molecule.",
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Ubiquitous presence of piscidin-1 in Atlantic cod as evidenced by immunolocalisation. / Ruangsri, J.; Fernandes, J.M.O.; Rombout, J.H.W.M.; Brinchmann, M.; Kiron, V.

In: BMC Veterinary Research, Vol. 8, 46, 2012.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Ubiquitous presence of piscidin-1 in Atlantic cod as evidenced by immunolocalisation

AU - Ruangsri, J.

AU - Fernandes, J.M.O.

AU - Rombout, J.H.W.M.

AU - Brinchmann, M.

AU - Kiron, V.

PY - 2012

Y1 - 2012

N2 - Background: Antimicrobial peptides (AMPs), the natural antibiotics bestowed upon all forms of life, consist of small molecular weight proteins with a broad spectrum antimicrobial activity against a variety of pathogenic microorganisms. Piscidins are one of the AMP families that are imperative for the innate defence mechanisms of teleosts. Atlantic cod, a basal fish belonging to the superorder Paracanthopterygii also possesses multiple piscidin peptides. Two piscidin paralogues (pis1 and pis2) and a novel alternative splice variant of pis2 of this fish were previously described by us. To shed light on other potent roles of these molecules, now we have mapped the distribution of piscidin 1 (Pis1), in different tissues and organs of cod through immunohistochemistry (IHC) employing an affinity purified polyclonal antibody specific to Pis1. Results: Various cell types and tissues of Atlantic cod including those from the immune organs of naive fish are armed with Pis1 peptide. Different types of the blood leucocytes and phagocytic cells among the leucocytes examined gave a relatively strong indication of Pis1 immunopositivity. In addition, other cell types such as hematopoietic cells, epithelial cells and multi-granular cells located in the mucosal and hematopoietic tissues were also Pis1-immunoreactive. More interestingly, chondrocytes appear to produce Pis1 and this is the first report on the presence of an AMP in cartilage tissue of fish. Furthermore, Pis1 immunopositivity was detected in other tissues and organs of naive fish including neural tissues, exocrine and endocrine glands, compound gland cells, excretory kidney, intestinal and respiratory epithelial cells, swim bladder, skin and hypodermis layer, myosepta, liver, heart, eye and oocytes. Conclusions: Pis1 peptide is produced by various cell types located in different tissues and organs of Atlantic cod. It is present in all immune-related organs of naive fish and the elevated peptide expression following phagocytosis strongly suggest their involvement in innate defence. Further, its widespread occurrence in non-immune tissues and organs of apparently healthy fish implies that piscidin may have other functions in addition to its role as an immune effector molecule.

AB - Background: Antimicrobial peptides (AMPs), the natural antibiotics bestowed upon all forms of life, consist of small molecular weight proteins with a broad spectrum antimicrobial activity against a variety of pathogenic microorganisms. Piscidins are one of the AMP families that are imperative for the innate defence mechanisms of teleosts. Atlantic cod, a basal fish belonging to the superorder Paracanthopterygii also possesses multiple piscidin peptides. Two piscidin paralogues (pis1 and pis2) and a novel alternative splice variant of pis2 of this fish were previously described by us. To shed light on other potent roles of these molecules, now we have mapped the distribution of piscidin 1 (Pis1), in different tissues and organs of cod through immunohistochemistry (IHC) employing an affinity purified polyclonal antibody specific to Pis1. Results: Various cell types and tissues of Atlantic cod including those from the immune organs of naive fish are armed with Pis1 peptide. Different types of the blood leucocytes and phagocytic cells among the leucocytes examined gave a relatively strong indication of Pis1 immunopositivity. In addition, other cell types such as hematopoietic cells, epithelial cells and multi-granular cells located in the mucosal and hematopoietic tissues were also Pis1-immunoreactive. More interestingly, chondrocytes appear to produce Pis1 and this is the first report on the presence of an AMP in cartilage tissue of fish. Furthermore, Pis1 immunopositivity was detected in other tissues and organs of naive fish including neural tissues, exocrine and endocrine glands, compound gland cells, excretory kidney, intestinal and respiratory epithelial cells, swim bladder, skin and hypodermis layer, myosepta, liver, heart, eye and oocytes. Conclusions: Pis1 peptide is produced by various cell types located in different tissues and organs of Atlantic cod. It is present in all immune-related organs of naive fish and the elevated peptide expression following phagocytosis strongly suggest their involvement in innate defence. Further, its widespread occurrence in non-immune tissues and organs of apparently healthy fish implies that piscidin may have other functions in addition to its role as an immune effector molecule.

KW - gadus-morhua l.

KW - antimicrobial peptides

KW - sea-bass

KW - dicentrarchus-labrax

KW - vibrio-anguillarum

KW - immune-systems

KW - mast-cells

KW - fish

KW - expression

KW - crosstalk

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DO - 10.1186/1746-6148-8-46

M3 - Article

VL - 8

JO - BMC Veterinary Research

JF - BMC Veterinary Research

SN - 1746-6148

M1 - 46

ER -