The occurrence of programmed cell death in unicellular organisms is a subject that arouses great interest of theoreticians and experimental scientists. Already found evolutionarily conserved genes and metabolic pathways confirmed its existence in yeast, protozoa and even bacteria. In the yeast Saccharomyces cerevisiae, at least three main types of death are distinguished: apoptosis, necrosis and autophagy. Their classification suggested by the Nomenclature Committee on Cell Death initially based on the morphological characteristics has now been extended to include the measurable biochemical characteristics. Several laboratory methods previously used to detect the types of cell death of higher eucaryotes and later developed and successfully used for the analysis of yeast cells are here critically reviewed. Their advantages and limitations are described.
- monitoring autophagy
- programmed necrosis
- global analysis
- budding yeast
Wloch-Salamon, D. M., & Bem, A. E. (2013). Types of cell death and methods of their detection in yeast Saccharomyces cerevisiae. Journal of Applied Microbiology, 114(2), 287-298. https://doi.org/10.1111/jam.12024