Two maize Kip-related proteins differentially interact with, inhibit and are phosphorylated by cyclin D-cyclin-dependent kinase complexes

Silvia K. Godínez-Palma, Fernando R. Rosas-Bringas, Omar G. Rosas-Bringas, Elpidio García-Ramírez, Jorge Zamora-Zaragoza, Jorge M. Vázquez-Ramos*

*Corresponding author for this work

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6 Citations (Scopus)

Abstract

The family of maize Kip-related proteins (KRPs) has been studied and a nomenclature based on the relationship to rice KRP genes is proposed. Expression studies of KRP genes indicate that all are expressed at 24 h of seed germination but expression is differential in the different tissues of maize plantlets. Recombinant KRP1;1 and KRP4;2 proteins, members of different KRP classes, were used to study association to and inhibitory activity on different maize cyclin D (CycD)-cyclin-dependent kinase (CDK) complexes. Kinase activity in CycD2;2-CDK, CycD4;2-CDK, and CycD5;3- CDK complexes was inhibited by both KRPs; however, only KRP1;1 inhibited activity in the CycD6;1-CDK complex, not KRP4;2. Whereas KRP1;1 associated with either CycD2;2 or CycD6;1, and to cyclin-dependent kinase A (CDKA) recombinant proteins, forming ternary complexes, KRP4;2 bound CDKA and CycD2;2 but did not bind CycD6;1, establishing a differential association capacity. All CycD-CDK complexes included here phosphorylated both the retinoblastoma- related (RBR) protein and the two KRPs; interestingly, while KRP4;2 phosphorylated by the CycD2;2-CDK complex increased its inhibitory capacity, when phosphorylated by the CycD6;1-CDK complex the inhibitory capacity was reduced or eliminated. Evidence suggests that the phosphorylated residues in KRP4;2 may be different for every kinase, and this would influence its performance as a cyclin-CDK inhibitor.

Original languageEnglish
Pages (from-to)1585-1597
JournalJournal of Experimental Botany
Volume68
Issue number7
DOIs
Publication statusPublished - 2017

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Keywords

  • CDKs
  • Cyclins D
  • ICK/KRPs
  • Kinase inhibition
  • KRP phosphorylation
  • Zea mays

Cite this

Godínez-Palma, S. K., Rosas-Bringas, F. R., Rosas-Bringas, O. G., García-Ramírez, E., Zamora-Zaragoza, J., & Vázquez-Ramos, J. M. (2017). Two maize Kip-related proteins differentially interact with, inhibit and are phosphorylated by cyclin D-cyclin-dependent kinase complexes. Journal of Experimental Botany, 68(7), 1585-1597. https://doi.org/10.1093/jxb/erx054