Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

C. Aguilera-Galvez, N. Champouret, H. Rietman, X. Lin, D. Wouters, Z. Chu, J.D.G. Jones, J.H. Vossen, R.G.F. Visser, P.J. Wolters, V.G.A.A. Vleeshouwers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.
Original languageEnglish
Pages (from-to)105-115
JournalStudies in Mycology
Volume89
DOIs
Publication statusPublished - 1 Mar 2018

Fingerprint

Phytophthora infestans
vpr Genes
Solanum
Solanum tuberosum
potatoes
loci
genes
Chromosomes
Oomycetes
Central America
Peru
Disease Resistance
Genetic Loci
South America
chromosomes
North America
Mexico
gene interaction
Breeding
coevolution

Keywords

  • Avr gene
  • Co-evolution
  • Late blight
  • Phytophthora infestans
  • Potato
  • R gene
  • Resistance
  • Solanum

Cite this

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title = "Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato",
abstract = "Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘D{\'e}sir{\'e}e’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.",
keywords = "Avr gene, Co-evolution, Late blight, Phytophthora infestans, Potato, R gene, Resistance, Solanum",
author = "C. Aguilera-Galvez and N. Champouret and H. Rietman and X. Lin and D. Wouters and Z. Chu and J.D.G. Jones and J.H. Vossen and R.G.F. Visser and P.J. Wolters and V.G.A.A. Vleeshouwers",
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Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato. / Aguilera-Galvez, C.; Champouret, N.; Rietman, H.; Lin, X.; Wouters, D.; Chu, Z.; Jones, J.D.G.; Vossen, J.H.; Visser, R.G.F.; Wolters, P.J.; Vleeshouwers, V.G.A.A.

In: Studies in Mycology, Vol. 89, 01.03.2018, p. 105-115.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Two different R gene loci co-evolved with Avr2 of Phytophthora infestans and confer distinct resistance specificities in potato

AU - Aguilera-Galvez, C.

AU - Champouret, N.

AU - Rietman, H.

AU - Lin, X.

AU - Wouters, D.

AU - Chu, Z.

AU - Jones, J.D.G.

AU - Vossen, J.H.

AU - Visser, R.G.F.

AU - Wolters, P.J.

AU - Vleeshouwers, V.G.A.A.

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.

AB - Late blight, caused by the oomycete pathogen Phytophthora infestans, is the most devastating disease in potato. For sustainable management of this economically important disease, resistance breeding relies on the availability of resistance (R) genes. Such R genes against P. infestans have evolved in wild tuber-bearing Solanum species from North, Central and South America, upon co-evolution with cognate avirulence (Avr) genes. Here, we report how effectoromics screens with Avr2 of P. infestans revealed defense responses in diverse Solanum species that are native to Mexico and Peru. We found that the response to AVR2 in the Mexican Solanum species is mediated by R genes of the R2 family that resides on a major late blight locus on chromosome IV. In contrast, the response to AVR2 in Peruvian Solanum species is mediated by Rpi-mcq1, which resides on chromosome IX and does not belong to the R2 family. The data indicate that AVR2 recognition has evolved independently on two genetic loci in Mexican and Peruvian Solanum species, respectively. Detached leaf tests on potato cultivar ‘Désirée’ transformed with R genes from either the R2 or the Rpi-mcq1 locus revealed an overlapping, but distinct resistance profile to a panel of 18 diverse P. infestans isolates. The achieved insights in the molecular R – Avr gene interaction can lead to more educated exploitation of R genes and maximize the potential of generating more broad-spectrum, and potentially more durable control of the late blight disease in potato.

KW - Avr gene

KW - Co-evolution

KW - Late blight

KW - Phytophthora infestans

KW - Potato

KW - R gene

KW - Resistance

KW - Solanum

U2 - 10.1016/j.simyco.2018.01.002

DO - 10.1016/j.simyco.2018.01.002

M3 - Article

VL - 89

SP - 105

EP - 115

JO - Studies in Mycology

JF - Studies in Mycology

SN - 0166-0616

ER -