Two complementary introgression line libraries in C. elegans

Research output: Contribution to conferenceAbstractAcademic

Abstract

A quantitative genetics study is as good as the mapping population used.
One of the most powerful mapping population designs is the introgression
line (IL) design. ILs consist of a single genetic locus introgressed in an
otherwise homogeneous background. For the nematode C. elegans, one
such population is available, containing loci of Hawaii CB4856 in an N2
background. Here we present a complementary population, which contains
loci of N2 in a CB4856 background. The combination of both panels retains
the power of the IL design, while adding the possibility to study the effect
of the genetic background. We demonstrate the synergy of the combined IL
populations by measuring several heat-stress related traits in strains
covering the top of chromosome IV.
The ILs were created by backcrossing six N2 x CB4856 recombinant inbred
lines (RILs) with CB4856. We started with two backcrosses. First, a
CB4856 hermaphrodite was crossed with a RIL. Second, the F1 was
backcrossed to a CB4856 male. This crossing scheme assures that the
mitochondria are derived from CB4856 and the N2 peel/zeel
incompatibility locus on chromosome I can be removed. After these two
crosses, the strains were selected using 41 insertions-deletions between the
N2 and CB4856 genomes as markers and additional backcrosses were made
when needed. These steps resulted in a population of 129 strains covering
~97% of the C. elegans genome, with a median introgression size of 3.9
Mb. To demonstrate the complementarity of the N2-in-CB4856 ILs with the
CB4856-in-N2 ILs, two traits were measured in three strains of each IL
population: (i) resistance to heat stress, and (ii) transcriptional response to
heat stress. For these experiments, synchronized populations of ILs were
grown for 46 hours at 20oC and either grown for an additional 2 hours at
20oC or exposed to 35oC for 2 hours.
Construction of the genome-wide IL library and its rough genetic map has
laid the foundation for whole-genome trait mapping respective to the
genetic background. Future plans include measuring several life-history
traits in both complementary populations and the construction of a highresolution
genetic map by sequencing. The combination of both panels can
resolve complex trait architecture in relation to the genetic background.
Original languageEnglish
Number of pages2
Publication statusPublished - 30 Mar 2016
EventEvolutionary Biology of Caenorhabditis and other nematodes - Cold Spring Harbor Laboratory, Cold Spring Harbor, United States
Duration: 30 Mar 20162 Apr 2016

Conference

ConferenceEvolutionary Biology of Caenorhabditis and other nematodes
CountryUnited States
CityCold Spring Harbor
Period30/03/162/04/16

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introgression
genome
genetic background
loci
heat stress
chromosomes
backcrossing
quantitative genetics
Hawaii
stress response
Nematoda

Cite this

Sterken, M. G., van Creij, J., Riksen, J. A. G., Tan, B., Snoek, L. B., & Kammenga, J. E. (2016). Two complementary introgression line libraries in C. elegans. Abstract from Evolutionary Biology of Caenorhabditis and other nematodes, Cold Spring Harbor, United States.
Sterken, M.G. ; van Creij, Jelle ; Riksen, J.A.G. ; Tan, Beatrice ; Snoek, L.B. ; Kammenga, J.E. / Two complementary introgression line libraries in C. elegans. Abstract from Evolutionary Biology of Caenorhabditis and other nematodes, Cold Spring Harbor, United States.2 p.
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Sterken, MG, van Creij, J, Riksen, JAG, Tan, B, Snoek, LB & Kammenga, JE 2016, 'Two complementary introgression line libraries in C. elegans', Cold Spring Harbor, United States, 30/03/16 - 2/04/16, .

Two complementary introgression line libraries in C. elegans. / Sterken, M.G.; van Creij, Jelle; Riksen, J.A.G.; Tan, Beatrice; Snoek, L.B.; Kammenga, J.E.

2016. Abstract from Evolutionary Biology of Caenorhabditis and other nematodes, Cold Spring Harbor, United States.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Two complementary introgression line libraries in C. elegans

AU - Sterken, M.G.

AU - van Creij, Jelle

AU - Riksen, J.A.G.

AU - Tan, Beatrice

AU - Snoek, L.B.

AU - Kammenga, J.E.

PY - 2016/3/30

Y1 - 2016/3/30

N2 - A quantitative genetics study is as good as the mapping population used.One of the most powerful mapping population designs is the introgressionline (IL) design. ILs consist of a single genetic locus introgressed in anotherwise homogeneous background. For the nematode C. elegans, onesuch population is available, containing loci of Hawaii CB4856 in an N2background. Here we present a complementary population, which containsloci of N2 in a CB4856 background. The combination of both panels retainsthe power of the IL design, while adding the possibility to study the effectof the genetic background. We demonstrate the synergy of the combined ILpopulations by measuring several heat-stress related traits in strainscovering the top of chromosome IV.The ILs were created by backcrossing six N2 x CB4856 recombinant inbredlines (RILs) with CB4856. We started with two backcrosses. First, aCB4856 hermaphrodite was crossed with a RIL. Second, the F1 wasbackcrossed to a CB4856 male. This crossing scheme assures that themitochondria are derived from CB4856 and the N2 peel/zeelincompatibility locus on chromosome I can be removed. After these twocrosses, the strains were selected using 41 insertions-deletions between theN2 and CB4856 genomes as markers and additional backcrosses were madewhen needed. These steps resulted in a population of 129 strains covering~97% of the C. elegans genome, with a median introgression size of 3.9Mb. To demonstrate the complementarity of the N2-in-CB4856 ILs with theCB4856-in-N2 ILs, two traits were measured in three strains of each ILpopulation: (i) resistance to heat stress, and (ii) transcriptional response toheat stress. For these experiments, synchronized populations of ILs weregrown for 46 hours at 20oC and either grown for an additional 2 hours at20oC or exposed to 35oC for 2 hours.Construction of the genome-wide IL library and its rough genetic map haslaid the foundation for whole-genome trait mapping respective to thegenetic background. Future plans include measuring several life-historytraits in both complementary populations and the construction of a highresolutiongenetic map by sequencing. The combination of both panels canresolve complex trait architecture in relation to the genetic background.

AB - A quantitative genetics study is as good as the mapping population used.One of the most powerful mapping population designs is the introgressionline (IL) design. ILs consist of a single genetic locus introgressed in anotherwise homogeneous background. For the nematode C. elegans, onesuch population is available, containing loci of Hawaii CB4856 in an N2background. Here we present a complementary population, which containsloci of N2 in a CB4856 background. The combination of both panels retainsthe power of the IL design, while adding the possibility to study the effectof the genetic background. We demonstrate the synergy of the combined ILpopulations by measuring several heat-stress related traits in strainscovering the top of chromosome IV.The ILs were created by backcrossing six N2 x CB4856 recombinant inbredlines (RILs) with CB4856. We started with two backcrosses. First, aCB4856 hermaphrodite was crossed with a RIL. Second, the F1 wasbackcrossed to a CB4856 male. This crossing scheme assures that themitochondria are derived from CB4856 and the N2 peel/zeelincompatibility locus on chromosome I can be removed. After these twocrosses, the strains were selected using 41 insertions-deletions between theN2 and CB4856 genomes as markers and additional backcrosses were madewhen needed. These steps resulted in a population of 129 strains covering~97% of the C. elegans genome, with a median introgression size of 3.9Mb. To demonstrate the complementarity of the N2-in-CB4856 ILs with theCB4856-in-N2 ILs, two traits were measured in three strains of each ILpopulation: (i) resistance to heat stress, and (ii) transcriptional response toheat stress. For these experiments, synchronized populations of ILs weregrown for 46 hours at 20oC and either grown for an additional 2 hours at20oC or exposed to 35oC for 2 hours.Construction of the genome-wide IL library and its rough genetic map haslaid the foundation for whole-genome trait mapping respective to thegenetic background. Future plans include measuring several life-historytraits in both complementary populations and the construction of a highresolutiongenetic map by sequencing. The combination of both panels canresolve complex trait architecture in relation to the genetic background.

M3 - Abstract

ER -

Sterken MG, van Creij J, Riksen JAG, Tan B, Snoek LB, Kammenga JE. Two complementary introgression line libraries in C. elegans. 2016. Abstract from Evolutionary Biology of Caenorhabditis and other nematodes, Cold Spring Harbor, United States.