Abstract
A quantitative genetics study is as good as the mapping population used.
One of the most powerful mapping population designs is the introgression
line (IL) design. ILs consist of a single genetic locus introgressed in an
otherwise homogeneous background. For the nematode C. elegans, one
such population is available, containing loci of Hawaii CB4856 in an N2
background. Here we present a complementary population, which contains
loci of N2 in a CB4856 background. The combination of both panels retains
the power of the IL design, while adding the possibility to study the effect
of the genetic background. We demonstrate the synergy of the combined IL
populations by measuring several heat-stress related traits in strains
covering the top of chromosome IV.
The ILs were created by backcrossing six N2 x CB4856 recombinant inbred
lines (RILs) with CB4856. We started with two backcrosses. First, a
CB4856 hermaphrodite was crossed with a RIL. Second, the F1 was
backcrossed to a CB4856 male. This crossing scheme assures that the
mitochondria are derived from CB4856 and the N2 peel/zeel
incompatibility locus on chromosome I can be removed. After these two
crosses, the strains were selected using 41 insertions-deletions between the
N2 and CB4856 genomes as markers and additional backcrosses were made
when needed. These steps resulted in a population of 129 strains covering
~97% of the C. elegans genome, with a median introgression size of 3.9
Mb. To demonstrate the complementarity of the N2-in-CB4856 ILs with the
CB4856-in-N2 ILs, two traits were measured in three strains of each IL
population: (i) resistance to heat stress, and (ii) transcriptional response to
heat stress. For these experiments, synchronized populations of ILs were
grown for 46 hours at 20oC and either grown for an additional 2 hours at
20oC or exposed to 35oC for 2 hours.
Construction of the genome-wide IL library and its rough genetic map has
laid the foundation for whole-genome trait mapping respective to the
genetic background. Future plans include measuring several life-history
traits in both complementary populations and the construction of a highresolution
genetic map by sequencing. The combination of both panels can
resolve complex trait architecture in relation to the genetic background.
One of the most powerful mapping population designs is the introgression
line (IL) design. ILs consist of a single genetic locus introgressed in an
otherwise homogeneous background. For the nematode C. elegans, one
such population is available, containing loci of Hawaii CB4856 in an N2
background. Here we present a complementary population, which contains
loci of N2 in a CB4856 background. The combination of both panels retains
the power of the IL design, while adding the possibility to study the effect
of the genetic background. We demonstrate the synergy of the combined IL
populations by measuring several heat-stress related traits in strains
covering the top of chromosome IV.
The ILs were created by backcrossing six N2 x CB4856 recombinant inbred
lines (RILs) with CB4856. We started with two backcrosses. First, a
CB4856 hermaphrodite was crossed with a RIL. Second, the F1 was
backcrossed to a CB4856 male. This crossing scheme assures that the
mitochondria are derived from CB4856 and the N2 peel/zeel
incompatibility locus on chromosome I can be removed. After these two
crosses, the strains were selected using 41 insertions-deletions between the
N2 and CB4856 genomes as markers and additional backcrosses were made
when needed. These steps resulted in a population of 129 strains covering
~97% of the C. elegans genome, with a median introgression size of 3.9
Mb. To demonstrate the complementarity of the N2-in-CB4856 ILs with the
CB4856-in-N2 ILs, two traits were measured in three strains of each IL
population: (i) resistance to heat stress, and (ii) transcriptional response to
heat stress. For these experiments, synchronized populations of ILs were
grown for 46 hours at 20oC and either grown for an additional 2 hours at
20oC or exposed to 35oC for 2 hours.
Construction of the genome-wide IL library and its rough genetic map has
laid the foundation for whole-genome trait mapping respective to the
genetic background. Future plans include measuring several life-history
traits in both complementary populations and the construction of a highresolution
genetic map by sequencing. The combination of both panels can
resolve complex trait architecture in relation to the genetic background.
| Original language | English |
|---|---|
| Number of pages | 2 |
| Publication status | Published - 30 Mar 2016 |
| Event | Evolutionary Biology of Caenorhabditis and other nematodes - Cold Spring Harbor Laboratory, Cold Spring Harbor, United States Duration: 30 Mar 2016 → 2 Apr 2016 |
Conference
| Conference | Evolutionary Biology of Caenorhabditis and other nematodes |
|---|---|
| Country/Territory | United States |
| City | Cold Spring Harbor |
| Period | 30/03/16 → 2/04/16 |