Truncated hemoglobins in actinorhizal nodules of Datisca glomerata

K. Pawlowski, K.R. Jacobsen, N. Alloisio, R.F. Denison, M. Klein, J.D. Tjepkema, T. Winzer, A. Sirrenberg, C. Guan, A.M. Berry

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19 Citations (Scopus)

Abstract

Three types of hemoglobins exist in higher plants, symbiotic, non-symbiotic, and truncated hemoglobins. Symbiotic (class II) hemoglobins play a role in oxygen supply to intracellular nitrogen-fixing symbionts in legume root nodules, and in one case ( Parasponia Sp.), a non-symbiotic (class I) hemoglobin has been recruited for this function. Here we report the induction of a host gene, dgtrHB1, encoding a truncated hemoglobin in Frankia-induced nodules of the actinorhizal plant Datisca glomerata. Induction takes place specifically in cells infected by the microsymbiont, prior to the onset of bacterial nitrogen fixation. A bacterial gene (Frankia trHBO) encoding a truncated hemoglobin with O (2)-binding kinetics suitable for the facilitation of O (2) diffusion ( ) is also expressed in symbiosis. Nodule oximetry confirms the presence of a molecule that binds oxygen reversibly in D. glomerata nodules, but indicates a low overall hemoglobin concentration suggesting a local function. Frankia trHbO is likely to be responsible for this activity. The function of the D. glomerata truncated hemoglobin is unknown; a possible role in nitric oxide detoxification is suggested.
Original languageEnglish
Pages (from-to)776-785
JournalPlant Biology
Volume9
Issue number6
DOIs
Publication statusPublished - 2007

Keywords

  • soybean root-nodules
  • nitric-oxide
  • nitrogenase activity
  • oxygen diffusion
  • mycobacterium-tuberculosis
  • bradyrhizobium-japonicum
  • casuarina-actinorhizae
  • sinorhizobium-meliloti
  • symbiotic hemoglobin
  • arabidopsis-thaliana

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    Pawlowski, K., Jacobsen, K. R., Alloisio, N., Denison, R. F., Klein, M., Tjepkema, J. D., Winzer, T., Sirrenberg, A., Guan, C., & Berry, A. M. (2007). Truncated hemoglobins in actinorhizal nodules of Datisca glomerata. Plant Biology, 9(6), 776-785. https://doi.org/10.1055/s-2007-965258