Trinucleotide repeat microsatellite markers for Black Poplar (Populus nigra L.)

M.J.M. Smulders, J. van der Schoot, P. Arens, B. Vosman

    Research output: Contribution to journalArticleAcademicpeer-review

    121 Citations (Scopus)

    Abstract

    Using an enrichment procedure, we have cloned microsatellite repeats from black poplar (Populus nigra L.) and developed primers for microsatellite marker analysis. Ten primer pairs, mostly for trinucleotide repeats, produced polymorphic fragments in P. nigra. Some of them also showed amplification in other poplar species. (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, P. lasiocarpa). The best six loci were tested on 23 P. nigra genotypes collected across Europe. The microsatellites produced up to 12 alleles per locus in this set, with observed heterozygosity between 0.32 and 0.91.
    Original languageEnglish
    Pages (from-to)188-190
    JournalMolecular Ecology Notes
    Volume1
    Issue number3
    DOIs
    Publication statusPublished - 2001

    Fingerprint Dive into the research topics of 'Trinucleotide repeat microsatellite markers for Black Poplar (Populus nigra L.)'. Together they form a unique fingerprint.

  • Cite this