Trinucleotide repeat microsatellite markers for Black Poplar (Populus nigra L.)

M.J.M. Smulders; J. van der Schoot; P. Arens; B. Vosman

doi:10.1046/j.1471-8278.2001.00071.x

Trinucleotide repeat microsatellite markers for Black Poplar (Populus nigra L.)

M.J.M. Smulders, J. van der Schoot, P. Arens, B. Vosman

Research output: Contribution to journal › Article › Academic › peer-review

139 Citations (Scopus)

Abstract

Using an enrichment procedure, we have cloned microsatellite repeats from black poplar (Populus nigra L.) and developed primers for microsatellite marker analysis. Ten primer pairs, mostly for trinucleotide repeats, produced polymorphic fragments in P. nigra. Some of them also showed amplification in other poplar species. (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, P. lasiocarpa). The best six loci were tested on 23 P. nigra genotypes collected across Europe. The microsatellites produced up to 12 alleles per locus in this set, with observed heterozygosity between 0.32 and 0.91.

Original language	English
Pages (from-to)	188-190
Journal	Molecular Ecology Notes
Volume	1
Issue number	3
DOIs	https://doi.org/10.1046/j.1471-8278.2001.00071.x
Publication status	Published - 2001

Keywords

Allelic ladder
Genetic diversity
Sequence-tagged microsatellite site (STMS)
Simple sequence repeat (SSR)

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10.1046/j.1471-8278.2001.00071.x

https://edepot.wur.nl/39685Licence: Taverne

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title = "Trinucleotide repeat microsatellite markers for Black Poplar (Populus nigra L.)",

abstract = "Using an enrichment procedure, we have cloned microsatellite repeats from black poplar (Populus nigra L.) and developed primers for microsatellite marker analysis. Ten primer pairs, mostly for trinucleotide repeats, produced polymorphic fragments in P. nigra. Some of them also showed amplification in other poplar species. (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, P. lasiocarpa). The best six loci were tested on 23 P. nigra genotypes collected across Europe. The microsatellites produced up to 12 alleles per locus in this set, with observed heterozygosity between 0.32 and 0.91.",

keywords = "Allelic ladder, Genetic diversity, Sequence-tagged microsatellite site (STMS), Simple sequence repeat (SSR)",

author = "M.J.M. Smulders and {van der Schoot}, J. and P. Arens and B. Vosman",

year = "2001",

doi = "10.1046/j.1471-8278.2001.00071.x",

language = "English",

volume = "1",

pages = "188--190",

journal = "Molecular Ecology Notes",

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T1 - Trinucleotide repeat microsatellite markers for Black Poplar (Populus nigra L.)

AU - Smulders, M.J.M.

AU - van der Schoot, J.

AU - Arens, P.

AU - Vosman, B.

PY - 2001

Y1 - 2001

N2 - Using an enrichment procedure, we have cloned microsatellite repeats from black poplar (Populus nigra L.) and developed primers for microsatellite marker analysis. Ten primer pairs, mostly for trinucleotide repeats, produced polymorphic fragments in P. nigra. Some of them also showed amplification in other poplar species. (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, P. lasiocarpa). The best six loci were tested on 23 P. nigra genotypes collected across Europe. The microsatellites produced up to 12 alleles per locus in this set, with observed heterozygosity between 0.32 and 0.91.

AB - Using an enrichment procedure, we have cloned microsatellite repeats from black poplar (Populus nigra L.) and developed primers for microsatellite marker analysis. Ten primer pairs, mostly for trinucleotide repeats, produced polymorphic fragments in P. nigra. Some of them also showed amplification in other poplar species. (P. deltoides, P. tricocarpa, P. tremula, P. tremuloides, P. candicans, P. lasiocarpa). The best six loci were tested on 23 P. nigra genotypes collected across Europe. The microsatellites produced up to 12 alleles per locus in this set, with observed heterozygosity between 0.32 and 0.91.

KW - Allelic ladder

KW - Genetic diversity

KW - Sequence-tagged microsatellite site (STMS)

KW - Simple sequence repeat (SSR)

U2 - 10.1046/j.1471-8278.2001.00071.x

DO - 10.1046/j.1471-8278.2001.00071.x

M3 - Article

SN - 1471-8278

VL - 1

SP - 188

EP - 190

JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 3

ER -

Trinucleotide repeat microsatellite markers for Black Poplar (Populus nigra L.)

Abstract

Keywords

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