Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens

Tomoko Suzuki, Tomoe Murakami, Yoshihiro Takizumi, Hiroyuki Ishimaru, Daiki Kudo, Yoshihiro Takikawa, Yoshinori Matsuda, Koji Kakutani, Yuling Bai, Teruo Nonomura*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

The present study aimed to explore the possibility of using the type I trichomes of tomato (Solanum lycopersicum) to monitor the infection processes of powdery mildews by microscopy. Individual trichome cells of two tomato genotypes were inoculated with pathogenic and non-pathogenic powdery mildew species, Pseudoidium neolycopersici, Erysiphe trifoliorum and Podosphaera xanthii. On the trichome cells of the tomato cultivar Moneymaker, hyphae of the pathogenic Pseudoidium neolycopersici (isolates KTP-03 and KTP-04) grew vigorously; whereas hyphal growth of the non-pathogenic Erysiphe trifoliorum and Podosphaera xanthii ceased after appressorium formation, which was associated with papilla formation and hypersensitive cell death, respectively. Similar infection processes of the tested powdery mildews were seen in Moneymaker epidermal cells. Therefore, tomato trichomes are suitable for analysing, at individual cell level, the infection processes of different pathotypes of powdery mildews and for observing the cytological responses of plants by non-pathogenic powdery mildews. On the other hand, it was observed that both isolates KTP-03 and KTP-04 failed to produce conidiophores on the hyphae elongating on Moneymaker trichomes. Similarly, no conidiophores were produced on the hyphae elongating on trichomes of Solanum peruvianum LA2172, which is resistant to KTP-03 and susceptible to KTP-04. Interestingly, delayed cell death occurred in LA2172 epidermal cells, which were attacked by KTP-03 hyphae elongating from trichomes and conidiophores were formed on new hyphae growing from the leaf epidermal cells. Thus, leaf trichomes and epidermal cells of the wild tomato species LA2172 reacted differently to the avirulent isolate KTP-03.

Original languageEnglish
Pages (from-to)115-125
JournalEuropean Journal of Plant Pathology
Volume150
Issue number1
Early online date2017
DOIs
Publication statusPublished - Jan 2018

Keywords

  • Delayed cell death
  • Erysiphe trifoliorum
  • Hypersensitive cell death
  • Papilla formation
  • Podosphaera xanthii
  • Pseudoidium neolycopersici

Fingerprint Dive into the research topics of 'Trichomes: interaction sites of tomato leaves with biotrophic powdery mildew pathogens'. Together they form a unique fingerprint.

Cite this