Trichome Independent Resistance against Western Flower Thrips in Tomato

Johanna A. Bac-Molenaar, Selena Mol, Maarten G. Verlaan, Joke van Elven, Hye Kyong Kim, Peter G.L. Klinkhamer, Kirsten A. Leiss, Klaas Vrieling

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Western flower thrips (WFT) are a major pest on many crops, including tomato. Thrips cause yield losses, not only through feeding damage, but also by the transmission of viruses of which the Tomato Spotted Wilt Virus is the most important one. In cultivated tomato, genetic diversity is extremely low, and all commercial lines are susceptible to WFT. Several wild relatives are WFT resistant and these resistances are based on glandular trichome-derived traits. Introgression of these traits in cultivated lines did not lead to WFT resistant commercial varieties so far. In this study, we investigated WFT resistance in cultivated tomato using a F2 population derived from a cross between a WFT susceptible and a WFT resistant cultivated tomato line. We discovered that this WFT resistance is independent of glandular trichome density or trichome-derived volatile profiles and is associated with three QTLs on chromosomes 4, 5 and 10. Foliar metabolic profiles of F3 families with low and high WFT feeding damage were clearly different. We identified α-tomatine and a phenolic compound as potential defensive compounds. Their causality and interaction need further investigation. Because this study is based on cultivated tomato lines, our findings can directly be used in nowadays breeding programs.

LanguageEnglish
Pages1011-1024
JournalPlant & cell physiology
Volume60
Issue number5
DOIs
Publication statusPublished - May 2019

Fingerprint

Thysanoptera
Trichomes
Frankliniella occidentalis
Lycopersicon esculentum
trichomes
tomatoes
alpha-tomatine
Tospovirus
Thrips (Thripidae)
Tomato spotted wilt virus
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 5
virus transmission
wild relatives
Chromosomes, Human, Pair 4
Metabolome
introgression
Causality
phenolic compounds
quantitative trait loci

Keywords

  • Insect resistance
  • Metabolite profiles
  • QTL mapping
  • Tomato
  • Trichome-derived volatiles
  • α-Tomatine

Cite this

Bac-Molenaar, J. A., Mol, S., Verlaan, M. G., van Elven, J., Kim, H. K., Klinkhamer, P. G. L., ... Vrieling, K. (2019). Trichome Independent Resistance against Western Flower Thrips in Tomato. Plant & cell physiology, 60(5), 1011-1024. https://doi.org/10.1093/pcp/pcz018
Bac-Molenaar, Johanna A. ; Mol, Selena ; Verlaan, Maarten G. ; van Elven, Joke ; Kim, Hye Kyong ; Klinkhamer, Peter G.L. ; Leiss, Kirsten A. ; Vrieling, Klaas. / Trichome Independent Resistance against Western Flower Thrips in Tomato. In: Plant & cell physiology. 2019 ; Vol. 60, No. 5. pp. 1011-1024.
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Bac-Molenaar, JA, Mol, S, Verlaan, MG, van Elven, J, Kim, HK, Klinkhamer, PGL, Leiss, KA & Vrieling, K 2019, 'Trichome Independent Resistance against Western Flower Thrips in Tomato', Plant & cell physiology, vol. 60, no. 5, pp. 1011-1024. https://doi.org/10.1093/pcp/pcz018

Trichome Independent Resistance against Western Flower Thrips in Tomato. / Bac-Molenaar, Johanna A.; Mol, Selena; Verlaan, Maarten G.; van Elven, Joke; Kim, Hye Kyong; Klinkhamer, Peter G.L.; Leiss, Kirsten A.; Vrieling, Klaas.

In: Plant & cell physiology, Vol. 60, No. 5, 05.2019, p. 1011-1024.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Bac-Molenaar, Johanna A.

AU - Mol, Selena

AU - Verlaan, Maarten G.

AU - van Elven, Joke

AU - Kim, Hye Kyong

AU - Klinkhamer, Peter G.L.

AU - Leiss, Kirsten A.

AU - Vrieling, Klaas

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AB - Western flower thrips (WFT) are a major pest on many crops, including tomato. Thrips cause yield losses, not only through feeding damage, but also by the transmission of viruses of which the Tomato Spotted Wilt Virus is the most important one. In cultivated tomato, genetic diversity is extremely low, and all commercial lines are susceptible to WFT. Several wild relatives are WFT resistant and these resistances are based on glandular trichome-derived traits. Introgression of these traits in cultivated lines did not lead to WFT resistant commercial varieties so far. In this study, we investigated WFT resistance in cultivated tomato using a F2 population derived from a cross between a WFT susceptible and a WFT resistant cultivated tomato line. We discovered that this WFT resistance is independent of glandular trichome density or trichome-derived volatile profiles and is associated with three QTLs on chromosomes 4, 5 and 10. Foliar metabolic profiles of F3 families with low and high WFT feeding damage were clearly different. We identified α-tomatine and a phenolic compound as potential defensive compounds. Their causality and interaction need further investigation. Because this study is based on cultivated tomato lines, our findings can directly be used in nowadays breeding programs.

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KW - Metabolite profiles

KW - QTL mapping

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KW - Trichome-derived volatiles

KW - α-Tomatine

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SP - 1011

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JO - Plant and Cell Physiology

T2 - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 5

ER -

Bac-Molenaar JA, Mol S, Verlaan MG, van Elven J, Kim HK, Klinkhamer PGL et al. Trichome Independent Resistance against Western Flower Thrips in Tomato. Plant & cell physiology. 2019 May;60(5):1011-1024. https://doi.org/10.1093/pcp/pcz018