Trichococcus shcherbakoviae sp. nov., isolated from a laboratory-scale anaerobic EGSB bioreactor operated at low temperature

Sofiya Nikolaevna Parshina*, Nikolaos Strepis, Steven Aalvink, Alla N. Nozhevnikova, Alfons J.M. Stams, Diana Z. Sousa

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

A new species of the genus Trichococcus, strain Art1T, was isolated from a psychrotolerant syntrophic propionate-oxidizing consortium, obtained before from a low-temperature EGSB reactor fed with a mixture of VFAs (acetate, propionate and butyrate). The 16S rRNA gene sequence of strain Art1T was highly similar to those of other Trichococcus species (99.7-99.9 %) but digital DNA-DNA hybridization values were lower than those recommended for the delineation of a novel species, indicating that strain Art1T is a novel species of the genus Trichococcus. Cells of strain Art1T are non-motile cocci with a diameter of 0.5-2.0 µm and were observed singularly, in pairs, short chains and irregular conglomerates. Cells of Art1T stained Gram-positive and produced extracellular polymeric substances . Growth was optimal at pH 6-7.5 and cells could grow in a temperature range of from -2 to 30 °C (optimum 25-30 °C). Strain Art1T can degrade several carbohydrates, and the main products from glucose fermentation are lactate, acetate, formate and ethanol. The genomic DNA G+C content of strain Art1T is 46.7 %. The major components of the cellular fatty acids are C16 : 1 ω9c, C16 : 0 and C18 : 1 ω9c. Based on genomic and physiological characteristics of strain Art1T, a new species of the genus Trichococcus, Trichococcusshcherbakoviae, is proposed. The type strain of Trichococcusshcherbakoviae is Art1T (=DSM 107162T = VKM B-3260T).

Original languageEnglish
Pages (from-to)529-534
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume69
Issue number2
DOIs
Publication statusPublished - 1 Feb 2019

Keywords

  • anaerobic bioreactor
  • digital DNA–DNA hybridization
  • fermentation
  • psychrotolerant
  • Trichococcus shcherbakoviae

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  • Datasets

    Genome assembly of a novel psychrotolerance bacterium, Trichococcus ART1 .

    Parshina, S. (Creator), Strepis, N. (Creator), Aalvink, S. (Creator), Nozhevnikova, A. N. (Creator), Stams, F. (Creator) & Machado de Sousa, D. (Creator), Wageningen University, 13 Jun 2018

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