Trapping of root-knot nematodes by the adhesive hyphae-forming fungus Arthrobotrys oligospora

E. den Belder

    Research output: Thesisexternal PhD, WU

    Abstract

    <p>The present study addresses the ecology of a particular isolate of <em>Arthrobotrys oligospora</em> (CBS 289.82) in relation to its efficacy in controlling the root-knot nematode, <em>Meloidogyne hapla.</em><p>This isolate was selected because it differs from most nematode-trapping fungi in that it captures nematodes with adhesive hyphae without having to form complex trapping devices. This characteristic may make it a very useful biological control agent. An attempt was made to gain insight into the factors controlling its capture ability.<p><em>In vitro</em> experiments demonstrated that the <em>A. oligospora</em> (CBS 289.82) isolate was very effective in capturing <em>M.</em><em>hapla</em> and <em>M. incognita,</em> compared to the ability of other fungi with other trapping devices. Mobile juveniles were all caught by the hyphae within one hour and in some cases attachment occurred within the very first contact (chapter 2).<p>Electron microscopic observations revealed that attachment of juveniles of <em>Meloidogyne</em> spp. to hyphae is mediated by a layer of extracellular material, about 0.1 gm thick, on the hyphae (chapter 3). Such a layer was never observed in hyphae of fungal cultures to which nematodes were not added, suggesting that its presence depends on an interaction of the fungus with the nematode. The attachment of <em>Meloidogyne</em> second-stage juveniles was not affected by temperatures between 5 and 3O°C. However, at 15°C ring structure development and growth of trophic hyphae were strongly hampered, which suggest that under prevailing soil temperatures in temperate regions, ring structure development and growth of trophic hyphae may proceed slowly whereas trapping would continue to occur (chapter 4).<p>Furthermore, the nutritional conditions during growth of the fungus did not correlate with the rapidity of nematode-hypha attachment. The results also provide evidence that the trapping ability of the isolate tested continued for over more than 70 days (chapter 4).<p><em>Arthrobotrys oligospora</em> (CBS 289.82) covered dead, ruptured nematodes with a dense mycelium, whereas dead but otherwise intact nematodes were penetrated through the buccal cavity by a corkscrew-like structure and were subsequently colonized by trophic hyphae. Colonization of living second-stage juveniles by trophic hyphae following attachment and penetration was faster than colonization of dead second-stage juveniles. The addition of dead juveniles to a fungal colony prior to the addition of living juveniles did not affect attachment or the development of trophic hyphae through the live juveniles. However, one day after the addition of live juveniles, the proportion of live nematodes with ring structures was higher than when living and dead juveniles were added at the same time. The development of trophic hyphae in dead second-stage juveniles was delayed in the presence of live second-stage juveniles. The results refute the commonly held assumption that poor possibilities for saprophytic growth are a prerequisite for the formation of trapping devices and the predacious mode of feeding in the fungus (chapter <em></em> 5).<p>An important quality of fungi as potential biological control agents is their ability to form mycelium and capture structures in the soil at the place where their activity is desirable.<p>The establishment and capture activity of this isolate in a simple microcosm system at 20°C, <em></em> was compared to that of other fungi from the <em>Dactylaria</em> -complex. Direct microscopic observations in microcosms confirmed the attachment of mobile juveniles of <em>M. hapla</em> to hyphae of A. oligospora (chapter 6). Application of about 30 mm hyphal fragments of <em>A. oligospora</em> (CBS 289.82) <em></em> per gram soil resulted in 100-170 <em></em> in of hyphae per gram oven-dry soil at 20°C within 12 <em></em> days, a reduction of 90% in the number of living nematodes of <em>M. hapla</em> within one day after addition and the extermination of the nematodes within 10 days. In non-sterilized soil, the hyphae reached a total length of 10 in per gram oven-dry soil. This amount of hyphal mass was sufficient to reduce the number of nematodes by 70% <em></em> as compared to the control within 10 days after nematodes were added to the soil. At 13°C, similar results were obtained. Even at low densities, this isolate is effective. Notwithstanding their ability to form the most extensive mycelial mats of all fungi tested and despite the fairly large amounts of <em>M. hapla</em> added, nematode capture in both adhesive ring-forming fungi A. conoides (CBS 265.83) <em></em> and <em>A. oligospora</em> (ATCC 24927) <em></em> was zero or low. This supports other observations that the adhesive ring-forming fungi are inefficient (chapter 6).<p>In chapter 7 <em></em> the results on the ability of <em>A. oligospora</em> (CBS 289.82) <em></em> to capture root-knot nematodes presented in the previous chapters are evaluated. The mechanism of nematode trapping is also discussed from the perspective of screening nematode-trapping fungi and using the adhesive hyphae-forming fungi as biological control agents.
    Original languageEnglish
    QualificationDoctor of Philosophy
    Awarding Institution
    Supervisors/Advisors
    • Brussaard, Lijbert, Promotor
    • Henfling, J.W.D.M., Co-promotor, External person
    Award date20 May 1994
    Place of PublicationS.l.
    Publisher
    Print ISBNs9789054852810
    Publication statusPublished - 1994

    Fingerprint

    Arthrobotrys oligospora
    root-knot nematodes
    adhesives
    hyphae
    trapping
    fungi
    Nematoda
    Meloidogyne hapla
    biological control agents
    nematophagous fungi
    Meloidogyne
    soil
    ovens
    mycelium
    growth and development

    Keywords

    • biological control
    • fungi
    • biological control agents
    • plant pests
    • pratylenchus
    • heteroderidae
    • tylenchidae
    • deuteromycotina
    • meloidogyne hapla
    • moniliaceae

    Cite this

    @phdthesis{6dde00838abb4266ae548d9b368738a8,
    title = "Trapping of root-knot nematodes by the adhesive hyphae-forming fungus Arthrobotrys oligospora",
    abstract = "The present study addresses the ecology of a particular isolate of Arthrobotrys oligospora (CBS 289.82) in relation to its efficacy in controlling the root-knot nematode, Meloidogyne hapla.This isolate was selected because it differs from most nematode-trapping fungi in that it captures nematodes with adhesive hyphae without having to form complex trapping devices. This characteristic may make it a very useful biological control agent. An attempt was made to gain insight into the factors controlling its capture ability.In vitro experiments demonstrated that the A. oligospora (CBS 289.82) isolate was very effective in capturing M.hapla and M. incognita, compared to the ability of other fungi with other trapping devices. Mobile juveniles were all caught by the hyphae within one hour and in some cases attachment occurred within the very first contact (chapter 2).Electron microscopic observations revealed that attachment of juveniles of Meloidogyne spp. to hyphae is mediated by a layer of extracellular material, about 0.1 gm thick, on the hyphae (chapter 3). Such a layer was never observed in hyphae of fungal cultures to which nematodes were not added, suggesting that its presence depends on an interaction of the fungus with the nematode. The attachment of Meloidogyne second-stage juveniles was not affected by temperatures between 5 and 3O°C. However, at 15°C ring structure development and growth of trophic hyphae were strongly hampered, which suggest that under prevailing soil temperatures in temperate regions, ring structure development and growth of trophic hyphae may proceed slowly whereas trapping would continue to occur (chapter 4).Furthermore, the nutritional conditions during growth of the fungus did not correlate with the rapidity of nematode-hypha attachment. The results also provide evidence that the trapping ability of the isolate tested continued for over more than 70 days (chapter 4).Arthrobotrys oligospora (CBS 289.82) covered dead, ruptured nematodes with a dense mycelium, whereas dead but otherwise intact nematodes were penetrated through the buccal cavity by a corkscrew-like structure and were subsequently colonized by trophic hyphae. Colonization of living second-stage juveniles by trophic hyphae following attachment and penetration was faster than colonization of dead second-stage juveniles. The addition of dead juveniles to a fungal colony prior to the addition of living juveniles did not affect attachment or the development of trophic hyphae through the live juveniles. However, one day after the addition of live juveniles, the proportion of live nematodes with ring structures was higher than when living and dead juveniles were added at the same time. The development of trophic hyphae in dead second-stage juveniles was delayed in the presence of live second-stage juveniles. The results refute the commonly held assumption that poor possibilities for saprophytic growth are a prerequisite for the formation of trapping devices and the predacious mode of feeding in the fungus (chapter 5).An important quality of fungi as potential biological control agents is their ability to form mycelium and capture structures in the soil at the place where their activity is desirable.The establishment and capture activity of this isolate in a simple microcosm system at 20°C, was compared to that of other fungi from the Dactylaria -complex. Direct microscopic observations in microcosms confirmed the attachment of mobile juveniles of M. hapla to hyphae of A. oligospora (chapter 6). Application of about 30 mm hyphal fragments of A. oligospora (CBS 289.82) per gram soil resulted in 100-170 in of hyphae per gram oven-dry soil at 20°C within 12 days, a reduction of 90{\%} in the number of living nematodes of M. hapla within one day after addition and the extermination of the nematodes within 10 days. In non-sterilized soil, the hyphae reached a total length of 10 in per gram oven-dry soil. This amount of hyphal mass was sufficient to reduce the number of nematodes by 70{\%} as compared to the control within 10 days after nematodes were added to the soil. At 13°C, similar results were obtained. Even at low densities, this isolate is effective. Notwithstanding their ability to form the most extensive mycelial mats of all fungi tested and despite the fairly large amounts of M. hapla added, nematode capture in both adhesive ring-forming fungi A. conoides (CBS 265.83) and A. oligospora (ATCC 24927) was zero or low. This supports other observations that the adhesive ring-forming fungi are inefficient (chapter 6).In chapter 7 the results on the ability of A. oligospora (CBS 289.82) to capture root-knot nematodes presented in the previous chapters are evaluated. The mechanism of nematode trapping is also discussed from the perspective of screening nematode-trapping fungi and using the adhesive hyphae-forming fungi as biological control agents.",
    keywords = "biologische bestrijding, schimmels, organismen ingezet bij biologische bestrijding, plantenplagen, pratylenchus, heteroderidae, tylenchidae, deuteromycotina, meloidogyne hapla, moniliaceae, biological control, fungi, biological control agents, plant pests, pratylenchus, heteroderidae, tylenchidae, deuteromycotina, meloidogyne hapla, moniliaceae",
    author = "{den Belder}, E.",
    note = "WU thesis 1776 Proefschrift Wageningen",
    year = "1994",
    language = "English",
    isbn = "9789054852810",
    publisher = "Den Belder",

    }

    Trapping of root-knot nematodes by the adhesive hyphae-forming fungus Arthrobotrys oligospora. / den Belder, E.

    S.l. : Den Belder, 1994. 147 p.

    Research output: Thesisexternal PhD, WU

    TY - THES

    T1 - Trapping of root-knot nematodes by the adhesive hyphae-forming fungus Arthrobotrys oligospora

    AU - den Belder, E.

    N1 - WU thesis 1776 Proefschrift Wageningen

    PY - 1994

    Y1 - 1994

    N2 - The present study addresses the ecology of a particular isolate of Arthrobotrys oligospora (CBS 289.82) in relation to its efficacy in controlling the root-knot nematode, Meloidogyne hapla.This isolate was selected because it differs from most nematode-trapping fungi in that it captures nematodes with adhesive hyphae without having to form complex trapping devices. This characteristic may make it a very useful biological control agent. An attempt was made to gain insight into the factors controlling its capture ability.In vitro experiments demonstrated that the A. oligospora (CBS 289.82) isolate was very effective in capturing M.hapla and M. incognita, compared to the ability of other fungi with other trapping devices. Mobile juveniles were all caught by the hyphae within one hour and in some cases attachment occurred within the very first contact (chapter 2).Electron microscopic observations revealed that attachment of juveniles of Meloidogyne spp. to hyphae is mediated by a layer of extracellular material, about 0.1 gm thick, on the hyphae (chapter 3). Such a layer was never observed in hyphae of fungal cultures to which nematodes were not added, suggesting that its presence depends on an interaction of the fungus with the nematode. The attachment of Meloidogyne second-stage juveniles was not affected by temperatures between 5 and 3O°C. However, at 15°C ring structure development and growth of trophic hyphae were strongly hampered, which suggest that under prevailing soil temperatures in temperate regions, ring structure development and growth of trophic hyphae may proceed slowly whereas trapping would continue to occur (chapter 4).Furthermore, the nutritional conditions during growth of the fungus did not correlate with the rapidity of nematode-hypha attachment. The results also provide evidence that the trapping ability of the isolate tested continued for over more than 70 days (chapter 4).Arthrobotrys oligospora (CBS 289.82) covered dead, ruptured nematodes with a dense mycelium, whereas dead but otherwise intact nematodes were penetrated through the buccal cavity by a corkscrew-like structure and were subsequently colonized by trophic hyphae. Colonization of living second-stage juveniles by trophic hyphae following attachment and penetration was faster than colonization of dead second-stage juveniles. The addition of dead juveniles to a fungal colony prior to the addition of living juveniles did not affect attachment or the development of trophic hyphae through the live juveniles. However, one day after the addition of live juveniles, the proportion of live nematodes with ring structures was higher than when living and dead juveniles were added at the same time. The development of trophic hyphae in dead second-stage juveniles was delayed in the presence of live second-stage juveniles. The results refute the commonly held assumption that poor possibilities for saprophytic growth are a prerequisite for the formation of trapping devices and the predacious mode of feeding in the fungus (chapter 5).An important quality of fungi as potential biological control agents is their ability to form mycelium and capture structures in the soil at the place where their activity is desirable.The establishment and capture activity of this isolate in a simple microcosm system at 20°C, was compared to that of other fungi from the Dactylaria -complex. Direct microscopic observations in microcosms confirmed the attachment of mobile juveniles of M. hapla to hyphae of A. oligospora (chapter 6). Application of about 30 mm hyphal fragments of A. oligospora (CBS 289.82) per gram soil resulted in 100-170 in of hyphae per gram oven-dry soil at 20°C within 12 days, a reduction of 90% in the number of living nematodes of M. hapla within one day after addition and the extermination of the nematodes within 10 days. In non-sterilized soil, the hyphae reached a total length of 10 in per gram oven-dry soil. This amount of hyphal mass was sufficient to reduce the number of nematodes by 70% as compared to the control within 10 days after nematodes were added to the soil. At 13°C, similar results were obtained. Even at low densities, this isolate is effective. Notwithstanding their ability to form the most extensive mycelial mats of all fungi tested and despite the fairly large amounts of M. hapla added, nematode capture in both adhesive ring-forming fungi A. conoides (CBS 265.83) and A. oligospora (ATCC 24927) was zero or low. This supports other observations that the adhesive ring-forming fungi are inefficient (chapter 6).In chapter 7 the results on the ability of A. oligospora (CBS 289.82) to capture root-knot nematodes presented in the previous chapters are evaluated. The mechanism of nematode trapping is also discussed from the perspective of screening nematode-trapping fungi and using the adhesive hyphae-forming fungi as biological control agents.

    AB - The present study addresses the ecology of a particular isolate of Arthrobotrys oligospora (CBS 289.82) in relation to its efficacy in controlling the root-knot nematode, Meloidogyne hapla.This isolate was selected because it differs from most nematode-trapping fungi in that it captures nematodes with adhesive hyphae without having to form complex trapping devices. This characteristic may make it a very useful biological control agent. An attempt was made to gain insight into the factors controlling its capture ability.In vitro experiments demonstrated that the A. oligospora (CBS 289.82) isolate was very effective in capturing M.hapla and M. incognita, compared to the ability of other fungi with other trapping devices. Mobile juveniles were all caught by the hyphae within one hour and in some cases attachment occurred within the very first contact (chapter 2).Electron microscopic observations revealed that attachment of juveniles of Meloidogyne spp. to hyphae is mediated by a layer of extracellular material, about 0.1 gm thick, on the hyphae (chapter 3). Such a layer was never observed in hyphae of fungal cultures to which nematodes were not added, suggesting that its presence depends on an interaction of the fungus with the nematode. The attachment of Meloidogyne second-stage juveniles was not affected by temperatures between 5 and 3O°C. However, at 15°C ring structure development and growth of trophic hyphae were strongly hampered, which suggest that under prevailing soil temperatures in temperate regions, ring structure development and growth of trophic hyphae may proceed slowly whereas trapping would continue to occur (chapter 4).Furthermore, the nutritional conditions during growth of the fungus did not correlate with the rapidity of nematode-hypha attachment. The results also provide evidence that the trapping ability of the isolate tested continued for over more than 70 days (chapter 4).Arthrobotrys oligospora (CBS 289.82) covered dead, ruptured nematodes with a dense mycelium, whereas dead but otherwise intact nematodes were penetrated through the buccal cavity by a corkscrew-like structure and were subsequently colonized by trophic hyphae. Colonization of living second-stage juveniles by trophic hyphae following attachment and penetration was faster than colonization of dead second-stage juveniles. The addition of dead juveniles to a fungal colony prior to the addition of living juveniles did not affect attachment or the development of trophic hyphae through the live juveniles. However, one day after the addition of live juveniles, the proportion of live nematodes with ring structures was higher than when living and dead juveniles were added at the same time. The development of trophic hyphae in dead second-stage juveniles was delayed in the presence of live second-stage juveniles. The results refute the commonly held assumption that poor possibilities for saprophytic growth are a prerequisite for the formation of trapping devices and the predacious mode of feeding in the fungus (chapter 5).An important quality of fungi as potential biological control agents is their ability to form mycelium and capture structures in the soil at the place where their activity is desirable.The establishment and capture activity of this isolate in a simple microcosm system at 20°C, was compared to that of other fungi from the Dactylaria -complex. Direct microscopic observations in microcosms confirmed the attachment of mobile juveniles of M. hapla to hyphae of A. oligospora (chapter 6). Application of about 30 mm hyphal fragments of A. oligospora (CBS 289.82) per gram soil resulted in 100-170 in of hyphae per gram oven-dry soil at 20°C within 12 days, a reduction of 90% in the number of living nematodes of M. hapla within one day after addition and the extermination of the nematodes within 10 days. In non-sterilized soil, the hyphae reached a total length of 10 in per gram oven-dry soil. This amount of hyphal mass was sufficient to reduce the number of nematodes by 70% as compared to the control within 10 days after nematodes were added to the soil. At 13°C, similar results were obtained. Even at low densities, this isolate is effective. Notwithstanding their ability to form the most extensive mycelial mats of all fungi tested and despite the fairly large amounts of M. hapla added, nematode capture in both adhesive ring-forming fungi A. conoides (CBS 265.83) and A. oligospora (ATCC 24927) was zero or low. This supports other observations that the adhesive ring-forming fungi are inefficient (chapter 6).In chapter 7 the results on the ability of A. oligospora (CBS 289.82) to capture root-knot nematodes presented in the previous chapters are evaluated. The mechanism of nematode trapping is also discussed from the perspective of screening nematode-trapping fungi and using the adhesive hyphae-forming fungi as biological control agents.

    KW - biologische bestrijding

    KW - schimmels

    KW - organismen ingezet bij biologische bestrijding

    KW - plantenplagen

    KW - pratylenchus

    KW - heteroderidae

    KW - tylenchidae

    KW - deuteromycotina

    KW - meloidogyne hapla

    KW - moniliaceae

    KW - biological control

    KW - fungi

    KW - biological control agents

    KW - plant pests

    KW - pratylenchus

    KW - heteroderidae

    KW - tylenchidae

    KW - deuteromycotina

    KW - meloidogyne hapla

    KW - moniliaceae

    M3 - external PhD, WU

    SN - 9789054852810

    PB - Den Belder

    CY - S.l.

    ER -