Transposon tagging : towards the isolation of the resistance R-genes in potato against Phytophthora infestans (Mont.) de Bary

A. El Kharbotly

Research output: Thesisinternal PhD, WU

Abstract

<p>The thesis describes the first step of the research leading to transposon tagging of the resistance genes against late blight of potato <em>(Phytophthora infestans).</em> Different diploid potato populations have been developed in which four <em>R</em> -genes have been localized on the RFLP map. Screening for high regeneration and transformation competence in a segregating population showed positive correlation between the <em>R1</em> gene and the competence for transformation. It resulted also in the selection of a diploid potato clone suitable for transposon tagging. The transformation of this clone with <em>Ds</em> -carrying T- DNA and the localization of 60 different T-DNA insertions were carried out. The involvement of other genetic factors in the expression of the <em>R</em> -genes was detected.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Jacobsen, Evert, Promotor
  • Pereira, A., Promotor, External person
Award date9 Oct 1995
Place of PublicationS.l.
Publisher
Print ISBNs9789054854548
Publication statusPublished - 1995

Keywords

  • plant breeding
  • disease resistance
  • pest resistance
  • solanum tuberosum
  • potatoes
  • plant pathogenic fungi
  • plant protection
  • fungicides
  • phytophthora
  • genetic engineering
  • recombinant dna

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